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Extraction method, purification method and application of alkaloids in ground beetles

An extraction method and a purification method technology, which are applied in the field of extraction and purification of alkaloids in the soft-shelled soft-shelled turtle, can solve problems such as the substance that cannot confirm the essential function of the soft-shelled turtle, the research on active ingredients is not clear, and the active ingredients of the soft-shelled turtle are not clear.

Inactive Publication Date: 2018-11-30
GUANGDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research on the active ingredients in the soft-shelled soft-shelled turtle is not clear, and it is impossible to confirm the substances that really play a substantial role in the soft-shelled turtle, which severely limits the clinical application of the soft-shelled soft-shelled turtle.
[0005] Therefore, to develop an extraction, purification method and application of alkaloids in the soft-shelled turtle, which is used to solve the technical defects in the prior art that the active ingredients in the soft-shelled turtle are not clear and severely limit its application, has become an urgent need for those skilled in the art to solve question

Method used

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  • Extraction method, purification method and application of alkaloids in ground beetles
  • Extraction method, purification method and application of alkaloids in ground beetles
  • Extraction method, purification method and application of alkaloids in ground beetles

Examples

Experimental program
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Embodiment 1

[0039] This embodiment is the extraction method of total alkaloids in soft-shelled turtle.

[0040] Fresh soft-shelled turtles were washed with distilled water, dried at 45°C, ground into powder, and stored at low temperature at 4°C for later use.

[0041] Get 20g ground turtle powder and use 95% ethanol 200ml (material ratio 1:10), under the condition of 70 ℃, Soxhlet extraction for 8 hours, filter; Filter the filter residue with 70% methanol 200ml again (material ratio 1:10), in Under the condition of 70°C, Soxhlet extraction was performed for 8 hours, filtered, and the two extracts were combined.

[0042] After the extract was dried under reduced pressure, it was dissolved with 50 ml of 0.5% dilute hydrochloric acid, then extracted with 150 ml of chloroform, and the extract was dried under reduced pressure to obtain 1.124 g of the total alkaloid components of the turtle with antibacterial and anticancer activities. The extraction yield was 5.62%.

Embodiment 2

[0044] In this example, 9-(2',2'-dimethylpropionylhydrazone)-3,6-dichloro-2,7-bis-[2-(diethylamine) was obtained after purification of the total alkaloids of the turtle - Specific examples of ethoxy]fluorene (GBA18).

[0045] (1), 5 g of total alkaloids of the turtle obtained in Example 1 were dissolved in 50 ml of 0.5% dilute hydrochloric acid, and the pH value was adjusted to 8.0 with dilute ammonia; then, extracted with 150 ml of chloroform, and the extract was dried under reduced pressure to obtain 89 mg of solid extract.

[0046] (2) Take 500g of 200-300 mesh silica gel, mix it with chloroform after activation and put it into a chromatographic column (3cm*50cm) by wet method. 89 mg of the solid extract obtained in step (1) was dissolved in chloroform and then wet-applied, eluted with chloroform / methanol (9:1), and the eluent was dried under reduced pressure to obtain 50 mg of the solid extract.

[0047] (3) Take 500 g of 200-300 mesh silica gel, mix it with chloroform af...

Embodiment 3

[0052] This embodiment is a specific embodiment of GBA18 carbon spectrum and hydrogen spectrum analysis. 3.1 1 H-NMR analysis

[0053] See further here Table 1 and figure 2 .

[0054] Table 1

[0055]

[0056]

[0057] 3.2 13 C-NMR analysis

[0058] See further here Table 2 and image 3 .

[0059] Table 2

[0060]

[0061]

[0062]

[0063]

[0064]

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Abstract

The invention belongs to the technical field of chemical extraction of traditional Chinese medicine, particularly relates to an extraction method, a purification method and application of alkaloids inground beetles, and specifically provides an extraction method for extracting the alkaloids from the ground beetles, a purification method for the alkaloids obtained according to the extraction method, application of the alkaloids obtained according to the extraction method or the purified alkaloids obtained according to the purification method, and application of the alkaloids obtained accordingto the extraction method or the purified alkaloids obtained according to the purification method in growth inhibition of gastric carcinoma cells. The extraction method has the advantages that GBA18 can be obtained through separation; experimental determination proves that the obtained products have a good inhibiting effect on gastric cancer and bacteria, and the half lethal dose and the minimum lethal dose are within the safety usage range recommended by the Chinese pharmacopoeia; the technical defects that active ingredients in the ground beetles are unclear in the prior art and applicationof the ground beetles is seriously limited are overcome.

Description

technical field [0001] The invention belongs to the technical field of chemical extraction of traditional Chinese medicines, and in particular relates to an extraction and purification method and application of alkaloids in soft-shelled turtles. Background technique [0002] Eupolyphages inensis, also known as Eupolyphages inensis, belongs to the insect class Hexapeda, the order Blattaria, and the family Corydiidae. The ground turtle mainly contains proteins, sugars, organic acids, steroids, alkaloids, amino acids, oils, terpene lactones, coumarins, phenols and other organic substances and various trace elements. It has a long history in the application field of traditional Chinese medicine. [0003] Modern biochemical molecules and pharmacological tests have proved that the soft-shelled turtle has anti-tumor effects. There have been a large number of domestic literature reports pointing out that the soft-shelled turtle has anti-mutation and anti-tumor effects, and it has b...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K31/15A61P31/04A61P31/10A61P35/00C07C251/84C07C249/16
CPCA61K31/15A61K35/63A61P31/04A61P31/10A61P35/00C07C249/16C07C2603/18C07C251/84Y02A50/30
Inventor 黄明星
Owner GUANGDONG UNIV OF TECH
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