Technology for preparing acrylamide through biochemical method
A technology of acrylamide and biochemical method, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as complex process, corrosion of production equipment, increased production cost, etc., achieve complete reaction, little environmental pollution, The effect of less waste
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Embodiment 1
[0019] a. Cultivation of strains: select high-quality strains, add them to the aqueous culture solution for preliminary cultivation, and the culture period is 24 hours, then add the strains after the preliminary cultivation to the culture medium composed of aqueous nutrient solution and pure water for a large amount Breeding, the breeding temperature is 25-37°C, the breeding cycle is 2-3 days, after the mass breeding is completed, take it out, put it in a centrifuge at a speed of 12000r / min, centrifuge for 30min, add pure water to dilute after centrifugation, and prepare a strain catalyst ;
[0020] B, the production of acrylamide: 0.08Kg of above-mentioned strain catalyst is joined in the acrylonitrile of 1Kg, carry out hydration reaction, after hydration reaction, reaction solution is filtered, then ion adsorption is carried out to filtrate, finally obtain acrylamide aqueous solution;
[0021] c. Refining extraction of acrylamide: the above-mentioned acrylamide aqueous solut...
Embodiment 2
[0024] a. Cultivation of strains: select high-quality strains, add them to the aqueous culture solution for preliminary cultivation, and the culture period is 24 hours, then add the strains after the preliminary cultivation to the culture medium composed of aqueous nutrient solution and pure water for a large amount Breeding, the breeding temperature is 25-37°C, the breeding cycle is 2-3 days, after the mass breeding is completed, take it out, put it in a centrifuge at a speed of 12000r / min, centrifuge for 30min, add pure water to dilute after centrifugation, and prepare a strain catalyst ;
[0025] B, the production of acrylamide: 0.12Kg of above-mentioned strain catalyst is joined in the acrylonitrile of 1Kg, carry out hydration reaction, after hydration reaction, reaction solution is filtered, then ion adsorption is carried out to filtrate, finally obtain acrylamide aqueous solution;
[0026] c. Refining extraction of acrylamide: the above-mentioned acrylamide aqueous solut...
Embodiment 3
[0029] a. Cultivation of strains: select high-quality strains, add them to the aqueous culture solution for preliminary cultivation, and the culture period is 24 hours, then add the strains after the preliminary cultivation to the culture medium composed of aqueous nutrient solution and pure water for a large amount Breeding, the breeding temperature is 25-37°C, the breeding cycle is 2-3 days, after the mass breeding is completed, take it out, put it in a centrifuge at a speed of 12000r / min, centrifuge for 30min, add pure water to dilute after centrifugation, and prepare a strain catalyst ;
[0030] B, the production of acrylamide: 0.1Kg of above-mentioned strain catalyst is joined in the acrylonitrile of 1Kg, carry out hydration reaction, after hydration reaction, reaction solution is filtered, then ion adsorption is carried out to filtrate, finally obtain acrylamide aqueous solution;
[0031] c. Refining extraction of acrylamide: the above-mentioned acrylamide aqueous soluti...
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