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Method used for measuring content of proteins in peony pollens

A protein content and pollen technology, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problem that there is no effective method for protein content determination in peony pollen, and achieve the effect of rapid determination.

Inactive Publication Date: 2018-11-30
TONGREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no effective method for the determination of protein content in peony pollen.

Method used

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Embodiment Construction

[0019] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] A method for measuring protein content in peony pollen, comprising the steps of: accurately weighing 1.0000g (±0.0001g) of peony pollen, transferring it into a 500ml dry digestion tube, adding 0.4gCuSO4 in each digestion tube in a fume hood and 6.0g K2SO4 and 20ml H2SO4, shake well, and place the digestion tube in a high-temperature digestion furnace for digestion. Pre-digest at 240°C for 30 minutes, then increase the temperature to 420°C for 90 minutes until the digestive juice is blue-green and transparent, take out the digestive tube and cool it to room temperature, complete the liquid addition and distillation in...

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Abstract

The invention discloses a method used for measuring content of proteins in peony pollens. The method comprises following steps: 1.0000g (+-0.0001g) of peony pollen is weighed accurately, and is introduced into a dried 500ml digestive tube, digestive tubes are introduced into a fume cupboard, 0.4g of CuSO4, 6.0g of K2SO4, and 20ml of H2SO4 are introduced into each digestive tube, the digestive tubes are shaken for uniform mixing, and the digestive tubes are introduced into a high temperature digestion furnace for 30min of digestion at 240 DEG C, the temperature is increased to 420 DEG C for another 90min of digestion until obtained digested solution is blue-green and is transparent, the digestive tubes are taken out from the high temperature digestion furnace for cooling to room temperature, in a full-automatic Kjeldahl apparatus, liquid feeding and distillation are carried out, and at last 0.1mol / L hydrochloric acid standard solution is adopted for titration to an end point; and at thesame time, reagent blank control test is carried out, and the content of proteins is calculated based on a formula.

Description

technical field [0001] The invention relates to the technical field of substance content determination methods, in particular to a method for protein content determination in peony pollen. Background technique [0002] Plant pollen is the essence of life and has high nutritional and medicinal value. It is rich in carbohydrates, proteins, vitamins, amino acids, mineral elements, lipids and other organic nutrients and enzymes, hormones, coenzymes, polypeptides, flavonoids, etc. active substance. It is a green and healthy new type of natural health care product and nourishing medicine, which has various functions such as improving brain function, regulating metabolism, improving body immunity, beautifying the skin and losing weight. [0003] The peony flower is large and the pollen output is large. At present, the annual output of peony pollen in my country is estimated to be thousands of tons, but the utilization rate of peony pollen is low, and a large amount of peony pollen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/415
Inventor 张孟琴郭宇婉陈仕学姚元勇卢忠英邢明明
Owner TONGREN UNIV