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Application of e3 ubiquitin ligase asb3 in the preparation of therapeutic drugs for liver cancer

A technology of ubiquitin ligase and therapeutic drugs, applied in the field of biomedicine, can solve the problems of curative effect and poor prognosis, achieve unique application value, block the growth cycle, and induce the effect of apoptosis of liver cancer cells

Active Publication Date: 2022-01-18
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a new non-surgical treatment method for liver cancer—using small interfering RNA to silence specific target genes. This treatment method is mainly to solve the problems of traditional liver cancer treatment methods, such as curative effect and poor prognosis.

Method used

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  • Application of e3 ubiquitin ligase asb3 in the preparation of therapeutic drugs for liver cancer
  • Application of e3 ubiquitin ligase asb3 in the preparation of therapeutic drugs for liver cancer
  • Application of e3 ubiquitin ligase asb3 in the preparation of therapeutic drugs for liver cancer

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Experimental program
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Effect test

Embodiment

[0040] 1. Experimental materials involved in in vitro experiments:

[0041] 1. Cell line: Human liver cancer cell line LM6 comes from the Cell Bank of the Chinese Academy of Sciences.

[0042] 2. Experimental animals: 5-week-old healthy female athymic nude mice purchased from Shanghai Animal Experiment Center.

[0043] 3. Experimental materials, reagents and kits

[0044] DMEM medium: Thermo Fisher Scientific Company; Opti-MEM medium: Thermo Fisher Scientific Company; fetal bovine serum: Thermo Fisher Scientific Company; propidium iodide: Thermo Fisher Scientific Company; : Shanghai Biyuntian Biotechnology Co., Ltd.; protease inhibitor cocktail: Sigma-Aldrich; ECL chromogenic solution: Beijing Kangwei Reagent Biotechnology Co., Ltd.; 30% acrylamide: Shanghai Beyotime Biotechnology Co., Ltd.; 1M Tris solution ( pH8.8 / pH6.6): Shanghai Beyotime Biotechnology Co., Ltd.; ammonium persulfate: Shanghai Beyotime Biotechnology Co., Ltd.; TEMED: Shanghai Beyotime Biotechnology Co., Lt...

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Abstract

The invention relates to the field of biomedicine, in particular to the application of E3 ubiquitin ligase ASB3 as a new anti-tumor target in the preparation of drugs for treating liver cancer. The invention provides a new treatment method for liver cancer by specifically down-regulating ASB3 with small interfering RNA. Interfering with ASB3 can inhibit the proliferation of liver cancer cells and induce apoptosis of liver cancer cells; it has a therapeutic effect on subcutaneous liver cancer implantation in nude mice; it solves the technical problems of poor curative effect and poor prognosis of the existing treatment methods for middle and advanced liver cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, specifically, the application of E3 ubiquitin ligase ASB3 as a new anti-tumor target in the treatment of liver cancer. Background technique [0002] Liver cancer is a common malignant tumor with a high incidence worldwide. The incidence and mortality of liver cancer have remained high for a long time, which has caused great harm to the lives and health of residents. GLOBOCAN's 2012 statistical report shows that the incidence of liver cancer in the world reached 782,451 in 2012, accounting for 5.6% of the total number of cancers in the world during the same period, and the world standardized incidence rate (ASR-W) was 10.1 / 100,000, ranking first among males, females and The 5th, 9th, and 7th ranks of the total cancer incidence; 384,770 cases were diagnosed in China, accounting for 12.9% of the total number of cancers in China during the same period, and the ASR-W was 22.3 / 100,000. Bits 2, 5,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K48/00A61K31/713A61P35/00
CPCA61K45/00A61P35/00A61K31/713C12Q1/6886C12Q2600/158
Inventor 杨冬琴车智慧张文丽刘福晨吴梦梦
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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