Method for efficient germination and seedling formation of blueberry seeds

A seed and blueberry technology, applied in the field of plant propagation, can solve the problems of hybrid seed loss, underdeveloped root system, crisp and tender texture, etc.

Active Publication Date: 2018-12-11
辽宁省果树科学研究所
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  • Abstract
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Problems solved by technology

[0004] To sum up, progress has been made in breaking dormancy and germination of blueberry seeds, and a certain number of hybrid seedlings can be obtained through the above methods. However, the seed germination rate of more than 10% to 40% is far from meeting the needs of blueberry breeding, resulting in Severe loss of extremely precious hybrid seed
Although the germination rate of the seeds can reach more than 80% by using the tissue culture method to carry out aseptic inoculation, it is found through the actual operation of this breeding laboratory that due to the small size of the blueberry seeds, the germinated seedlings are weak, crisp and tender, and have undeveloped root systems. In the process of transplanting to the matrix, it is very easy to damage and cause dead seedlings, the seedling rate is low, and the inoculation and transplanting are time-consuming and labor-intensive, and the operating cost is too high. For the thousands of blueberry hybrid seeds obtained by large-scale hybrid breeding The operability and feasibility of aseptic seeding in tissue culture are not high

Method used

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  • Method for efficient germination and seedling formation of blueberry seeds
  • Method for efficient germination and seedling formation of blueberry seeds
  • Method for efficient germination and seedling formation of blueberry seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Gibberellin treatment concentration screening

[0039] The present embodiment sets 0 (control), 12.5, 25, 50, 100, 200, 400, 8 treatments of 800 mg / L respectively to gibberellin concentration. Select plump and mature seeds of blueberry varieties 'Lanfeng', 'Beilu' and 'Duke' (fresh seeds, ready to use now), 100 grains / treatment. Seeds were wrapped in filter paper and soaked in gibberellin solution for 20 h, rinsed with water for later use, and crushed sphagnum moss (Sphagnummoss, imported from Chile, purchased from Qingzhou Dahan Agricultural Technology Co., Ltd.) was fully absorbed and spread in a foam box with holes at the bottom ( 30cm×50cm×15cm) (the seeding container is not limited, but the bottom needs to be permeable with holes), the thickness of the crushed sphagnum is about 7cm, the spare seeds are sown on the crushed sphagnum, and covered with 1cm thick crushed sphagnum (the same below) , cultivated in an artificial climate box, culture conditions are set: 12...

Embodiment 2

[0042] Storage method screening

[0043] There are 6 types of fresh seeds (control), fresh seeds stored at 4°C (relative humidity 80%, sealed), fresh seeds frozen at -20°C, naturally dried at room temperature, naturally dried at 4°C, and naturally dried at -20°C. Storage method, storage time 3-6 months. 'Northern land' seeds, 100 grains / treatment, soaked in 400mg / L gibberellin for 20h as the method of breaking dormancy to treat the seeds stored in different ways, and sow them in the crushed sphagnum moss substrate. The culture conditions are the same as in Example 1.

[0044] The present embodiment counts the germination rate of 40d and 60d after sowing, and analyzes the influence of storage methods on the germination rate of blueberry seeds. The results are shown in figure 2 . Depend on figure 2 It can be seen that the germination rate increases with the prolongation of the culture time, and the fresh seeds have the highest germination rate after being treated with gibb...

Embodiment 3

[0046] Matrix screening

[0047] In this embodiment, crushed sphagnum moss, peat, fine river sand, garden soil and mixed nutrient soil are set to the sowing substrate (peat: garden soil: decomposed cow dung / organic fertilizer=2:1:0.1, 1-1.5kg sulfur / m 3 ) for 5 treatments, select the fresh plump seeds of 'Hokulu', 100 grains / treatment, soak the seeds in 400mg / L gibberellin for 20h as the method of breaking dormancy, and then sow them into the above-mentioned 5 kinds of substrates respectively, and cultivate them for the first 3 months Condition embodiment 1, analyze the influence of substrate on germination rate according to germination rate, move to seedling-growing greenhouse to grow thereafter, take seedling plant height as index, judge the influence of substrate on seedling later stage growth (remark: blueberry seedling side branch occurrence amount is closely related to density Correlation, if the density is small, then the amount of lateral shoots will be large, in order...

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Abstract

The invention provides a method for efficiency germination and seedling formation of blueberry seeds, and belongs to the technical field of plant propagation. The method for efficiency germination andseedling formation of blueberry seeds comprises the steps of seed soaking, substrate composition and later-period management; a substrate used for seeding comprises two layers: the upper layer contains smashed sphagna with thickness of 3-10cm, and the lower layer contains nutrient oil with thickness of 5-15cm. Smashed sphagna is the optimal substrate for seeding of the blueberry seeds, and the germination rate of the blueberry seeds seeded into the smashed sphagna can reach more than 85%. The nutrient oil is the optimal substrate for the growth period of the blueberry seedlings, the growth vigour of the blueberry seedlings in the middle and later periods of growth is the best, 6-mouth blueberry seedlings can grow to 20cm in the nutrient oil, and 9-month blueberry seedlings can grow to 40cm in the nutrient oil.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for efficient germination and seedling formation of blueberry seeds. Background technique [0002] According to the 2017 China Blueberry Industry Annual Development Report, my country's blueberry cultivation area increased from 24hm2 in 2001 to 2 Increased to 46,891hm in 2017 2 , the output has increased from 0 to 114,905t, and the development speed has been particularly rapid in recent years. my country's blueberry production is almost entirely dependent on imported varieties. There is a lack of excellent new varieties with independent intellectual property rights, adapting to my country's complex climate conditions and being able to be popularized on a large scale. This is not compatible with my country's rapidly developing blueberry industry. Therefore, it is imminent to cultivate new blueberry varieties with independent intellectual property r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01G24/28A01G24/20A01G24/10A01G24/22
CPCA01C1/00A01G24/10A01G24/20A01G24/22A01G24/28
Inventor 刘有春刘成魏永祥杨艳敏王兴东王莉
Owner 辽宁省果树科学研究所
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