Preparation method of MnO2 carrier coated drug precursor 5-ALA

Abstract

The invention discloses a preparation method of a MnO2 carrier coated pharmaceutical precursor 5- ALA. The method comprises the following steps: first, dissolving 100 mg of 5-ALA in 2ml of deionized water to adjust the pH to 7 for storage; then putting 250mu L of 50 mg / ml 5-ALA, 14mg of EDC, 9.3mg of NHS and 4.8ml deionized water into a 25ml round-bottom flask, and reacting at room temperature for30min; then adding 0.486g of BSA, and reacting at room temperature for 12h; dissolving 14mg of MnCl2 and 4H2O in 1ml water, dropwise adding the obtained solution to the reaction solution obtained inthe above step, and adjusting the pH of the reaction solution to 11 with 1M NaOH; then transferring the reaction solution to a dialysis bag after 2h, dialyzing for 3 days in deionized water to obtaina final product, and storing the final product in a refrigerator at 4 DEG C. By using synthetic protein and drug molecular complex, and mineralizing multi-response type MnO2, the MnO2 carrier coated drug precursor not only can be used as a stabilizer to mineralize and synthesize MnO2, but also can be used as a carrier of 5-ALA to enhance the blood circulation time, thereby realizing enhanced photodynamic therapy.

Description

Technical field

[0001] The invention relates to the field of biomedical materials, in particular to a multi-response MnO 2 Preparation of carrier-coated drug precursor 5-ALA and its application in photodynamic enhancement. Background technique

[0002] Photodynamic therapy is a new method of tumor treatment. In simple terms, it is a method of irradiating a light source of a certain wavelength on the photosensitizer so that it can absorb light to convert local oxygen molecules into highly toxic reactive oxygen species, and induce cell apoptosis or necrosis. The photosensitizer, the light source and the oxygen at the treatment site have become the three essential components of photodynamic therapy. However, due to the high metabolic level of solid tumors, most tumor cells rely on anaerobic respiration to complete their metabolism, resulting in a hypoxic environment at the tumor cells, making photodynamic therapy dependent on oxygen content in a passive situation. However, the cha...

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