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Method for improving sugar content in strawberry fruit

A sugar content, strawberry technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of large gaps in fruit flavor quality, low sugar content in strawberry fruit, and poor flavor quality. Good and other questions

Active Publication Date: 2018-12-11
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, due to different varieties of strawberries, the quality of fruit flavor varies greatly.
The cultivated area and market share of varieties bred by conventional hybridization methods in my country are lower than those of Japanese and American varieties. One of the most important reasons is that the low sugar content of strawberry fruit leads to poor flavor quality.

Method used

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  • Method for improving sugar content in strawberry fruit
  • Method for improving sugar content in strawberry fruit
  • Method for improving sugar content in strawberry fruit

Examples

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Embodiment 1

[0045] (1) Method for Screening Strawberry Polyol / Monosaccharide Transporter Genes Based on cDNA Sequence 1. Strawberry Polyol / Monosaccharide Transporter Gene Mining. The whole strawberry genome (Fragaria vesca) published on the NCBI and Strawberry Genome website (http: / / www.strawberrygenome.org) uses the protein sequence of the Arabidopsis monosaccharide transporter PMT (polyol / monosaccharidetransporter) as a template, respectively for Strawberry The genomes were compared, and a total of 13 Arabidopsis AtPMT homologous suspected sugar transporter genes were obtained.

[0046] 2. Primer design. Using software Premier primers5.0, quantitative PCR primers were designed in the cDNA region of each sugar transporter gene, and 18SrRNA was used as an internal reference gene for fluorescent quantitative PCR analysis. Quantitative PCR primers (Table 1) were designed according to the cDNA sequence of the gene obtained above, and 18SrRNA was used as an internal reference gene.

[0047]...

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Abstract

The invention provides a method for improving the sugar content in strawberry fruit. The method comprises the steps that the gene of polyhydric alcohol / monosaccharide transport protein of a coded strawberry is transferred into a target strawberry fruit to conduct instant overexpression, and the strawberry fruit with the high sugar content is obtained; and the gene of polyhydric alcohol / monosaccharide transport protein of the coded strawberry is composed of the base sequence as shown in the sequence 1 in the sequence table. The method provided by the invention improves the sugar content in thestrawberry fruits, and the fructose content in the strawberry fruit is greatly improved compared with the control group, and the taste of the strawberry fruit is effectively improved.

Description

technical field [0001] The invention relates to the field of strawberry planting, in particular to a method for increasing the sugar content in strawberry fruit. Background technique [0002] Strawberry is a perennial herb of the family Rosaceae. The fruit is pointed and ovoid, delicious, red and tender, with juicy pulp. Because of its rich vitamin C and wide application value, it is known as the "Queen of Fruits". However, due to different varieties of strawberries, the quality of fruit flavor varies greatly. The cultivated area and market share of varieties bred by conventional hybridization methods in my country are lower than those of Japanese and American varieties. One of the most important reasons is that the low sugar content of strawberry fruit leads to poor flavor quality. How to increase the sugar content of strawberry fruit, improve the flavor quality of fruit, and increase market share have become technical problems demanding prompt solution. [0003] Sugar i...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/08A01H6/74
CPCC12N15/8245C07K14/415
Inventor 伊兴凯魏书
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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