Molecular marker of QTL (quantitative trait loci) loci in flowering period of maize chromosome 9 and application of molecular marker of QTL loci in flowering period of maize chromosome 9

Abstract

The invention relates to the technical field of biology, and discloses a molecular marker of QTL (quantitative trait loci) loci in a flowering period of a maize chromosome 9 and application of the QTLloci in the flowering period of the maize chromosome 9. The molecular marker comprises SEQ ID NO. 1 to 4. A BC1RIL population is used as a material to identify a QTL locus of the maize flowering period between InDel markers JAAS4374 and JAAS4375 on a maize chromosome 9 by genomics analysis, molecular marker development, phenotypic identification, QTL analysis and a series of processes. The locuscan explain 10.4% of phenotypic variation in a silking period, and can explain 3.4% of phenotypic variation in a pollinating period at the same time, and the locus is a stably expressed locus which controls the flowering period of maize and has pleiotropy. The analysis shows that the marker can be used for prediction and selection of the flowering period of maize.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker of a QTL locus in the flowering stage of the No. 9 chromosome of maize and an application thereof. Background technique [0002] Corn is one of the three most important crops in the world, and it is also the main three-way crop of grain, economics and feed in my country. Before 2010, the sown area and total output both ranked second. In 2014, the sown area of ​​corn in my country reached 36.2 million hectares. Since then, it has become the largest food crop in my country and occupies a very important position in China's agricultural production and national economic development. [0003] In recent years, due to factors such as land transfer, large-scale operation, and urbanization construction, as well as the transfer of young and middle-aged laborers from rural areas to cities, corn production management has been extensive and backward, with low returns. Many rea...

AI Technical Summary

Problems solved by technology

Compared with Arabidopsis and rice, on the one hand, the maize genome is larger, with more repetitive sequences, and the genome structure is more complex; Susceptible to environmental influences, cloning is difficult

In addition, limited by the type of mapping population, the type of markers used, the accuracy of the genetic linkage map, and environmental control, there is no consistent result for the QTL analysis of maize flowering period.

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