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Detection method of viable bacterium number of embedded probiotic product

A detection method and technology of probiotics, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as large detection errors and unscientific results, achieve small counting errors, reliable detection results, and improve stability Effect

Active Publication Date: 2018-12-14
广州富诺营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for detecting the number of viable bacteria in embedded probiotic products, which solves the problems of large detection errors and unscientific results in the prior art

Method used

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  • Detection method of viable bacterium number of embedded probiotic product
  • Detection method of viable bacterium number of embedded probiotic product

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for detecting the number of viable bacteria in an embedded probiotic product, comprising the following steps:

[0030] (1) Pretreatment: Weigh an appropriate amount of the embedded probiotic product to be tested and grind it into a fine powder in a sterile mortar;

[0031] (2) Preparation of bacterial suspension: Weigh 1 mg of the fine powder prepared in step (1), add it to a 50ml centrifuge tube, add 30ml of dilution buffer, vortex shaker to fully dissolve the embedding layer, and obtain bacterial suspension liquid;

[0032] (3) Gradient dilution: oscillate and mix the bacterial suspension prepared in step (2), take 1ml of the mixed bacterial suspension, add dilution buffer to dilute into 4 concentration gradients, and the corresponding dilution factor is 5 times , 50 times, 500 times, 5000 times;

[0033] (4) Bacterial colony culture: draw the gradient dilution in step (3) and add in the sterile petri dish, add MRS culture medium and mix, leave standstill, ...

Embodiment 2

[0044] A method for detecting the number of viable bacteria in an embedded probiotic product, comprising the following steps:

[0045] (1) Pretreatment: Weigh an appropriate amount of the embedded probiotic product to be tested and grind it into a fine powder in a sterile mortar;

[0046] (2) Preparation of bacterial suspension: Weigh 3mg of the fine powder prepared in step (1), add it to a 50ml centrifuge tube, add 40ml of dilution buffer, vortex shaker to fully dissolve the embedding layer, and obtain bacterial suspension liquid;

[0047] (3) Gradient dilution: Shake and mix the bacterial suspension prepared in step (2), take 3ml of the mixed bacterial suspension, add dilution buffer to dilute into 4 concentration gradients, and the corresponding dilution factor is 10 times , 100 times, 1000 times, 10000 times;

[0048] (4) Bacterial colony culture: draw the gradient dilution in step (3) and add in the sterile petri dish, add MRS culture medium and mix, leave standstill, a...

Embodiment 3

[0059] A method for detecting the number of viable bacteria in an embedded probiotic product, comprising the following steps:

[0060] (1) Pretreatment: Weigh an appropriate amount of the embedded probiotic product to be tested and grind it into a fine powder in a sterile mortar;

[0061] (2) Preparation of bacterial cell suspension: Weigh 5 mg of the fine powder prepared in step (1), add it to a 50 ml centrifuge tube, add 50 ml of dilution buffer, vortex shaker to fully dissolve the embedding layer, and obtain the bacterial cell suspension liquid;

[0062] (3) Gradient dilution: oscillate and mix the bacterial suspension obtained in step (2), take 5ml of the mixed bacterial suspension, add dilution buffer to dilute into 4 concentration gradients, and the corresponding dilution factor is 15 times , 150 times, 1500 times, 15000 times;

[0063] (4) Bacterial colony culture: draw the gradient dilution in step (3) and add in the sterile petri dish, add MRS culture medium and mix...

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Abstract

The invention discloses a detection method of the viable bacterium number of an embedded probiotic product and relates to the technical field of probiotic products. The method comprises the steps as follows: (1) pretreatment; (2) preparation of a thallus suspension; (3) gradient dilution; (4) bacterial colony culture; (5) bacterial colony counting: performing bacterial colony counting on a culturedish in the step (4) to obtain a viable bacterium detection result, wherein a dilution buffer solution in the step (2) is prepared from substances in percentage by weight as follows: 0.3%-0.6% of KH2PO4, 0.5%-0.7% of Na2HPO4, 0.6%-1.0% of NaCl, 0.5%-1.5% of tween-80, 0.05%-0.1% of polyethylene glycol, 0.01%-0.1% of polyoxypropylene glycerol ether, 0.04%0.08% of beta-cyclodextrine and the balanceof purified water. The tween-80 is used as a surfactant to promote dissolution of an embedded layer and completely release probiotics, the added polyoxypropylene glycerol ether and beta-cyclodextrinecan eliminate foam and foreign odors, the counting error is small, the detection result is reliable, strict sterile operation is realized, mixed bacterium pollution is avoided, the detection result credibility is high, and the promotional value is very high.

Description

technical field [0001] The invention relates to the technical field of probiotic products, in particular to a method for detecting the number of viable bacteria in embedded probiotic products. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects to improve the host's micro-ecological balance and exert beneficial effects. Beneficial bacteria or fungi in humans and animals mainly include Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, and yeasts. At present, the most powerful products researched in the world are mainly compound active probiotics composed of the above-mentioned various microorganisms, which are widely used in the fields of bioengineering, industry and agriculture, food safety, and life a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 程彦邓颖妍
Owner 广州富诺营养科技有限公司
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