Specific yolk immunoglobulin composition and preparation thereof

A technology of egg yolk immunoglobulin and composition, which is applied in the field of specific egg yolk immunoglobulin composition and its preparation to achieve good killing effect, solve the problem of pan-drug resistance, and have the effects of strong specificity

Inactive Publication Date: 2018-12-18
GUANGZHOU PHGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing IgY is usually based on a single antibody, and in many cases, due to the patient's underlying disease and the severity and persistence of the disease, the pathogenic bacteria are often diversified, and mixed infections are often the main type, so IgY with a single antibody is used doesn't solve the problem very well

Method used

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  • Specific yolk immunoglobulin composition and preparation thereof
  • Specific yolk immunoglobulin composition and preparation thereof
  • Specific yolk immunoglobulin composition and preparation thereof

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preparation example Construction

[0016] Further, the preparation method of the specific yolk immunoglobulin composition comprises:

[0017] S1. Mix the inactivated Acinetobacter baumannii, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, cluster A and type B hemolytic streptococcus evenly to make a composite antigen.

[0018] Wherein, according to parts by weight, the composite antigen includes 20 to 25 parts of inactivated Acinetobacter baumannii, 15 to 20 parts of Staphylococcus aureus, 10 to 15 parts of Escherichia coli, 10 to 15 parts of Pseudomonas aeruginosa, 10 to 15 copies of Candida albicans, 25 to 35 copies of cluster A and type B hemolytic streptococci.

[0019] Preferably, in parts by weight, the composite antigen includes 21-23 parts of inactivated Acinetobacter baumannii, 17-20 parts of Staphylococcus aureus, 12-14 parts of Escherichia coli, and 12-14 parts of Pseudomonas aeruginosa , 10 to 13 copies of Candida albicans, 28 to 32 copies of cluster A and type B ...

Embodiment 1

[0039] This embodiment provides a specific yolk immunoglobulin composition, the preparation method of which is as follows:

[0040] S1. Select six pathogenic bacteria, Acinetobacter baumannii, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, group A and type B hemolytic streptococcus, and use LB medium at 38°C respectively Cultivate in a three-gas incubator for 18 hours; then separate the above strains and inoculate them into broth medium, and cultivate them in a constant temperature double-layer shaking incubator at 38°C for 23 hours. The speed of the constant temperature shaking incubator is 200rpm. Then inactivate it for later use.

[0041] The six pathogenic bacteria after inactivation treatment were divided into 25 parts of Acinetobacter baumannii, 20 parts of Staphylococcus aureus, 10 parts of E. Mix 25 parts of streptococcus sexually to obtain a strain mixture.

[0042] Dissolve the mixed strain mixture in 0.1mol / L PBS solution to pre...

Embodiment 2

[0046] This embodiment provides a specific yolk immunoglobulin composition, the preparation method of which is as follows:

[0047] S1. Select six pathogenic bacteria, Acinetobacter baumannii, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, group A and type B hemolytic streptococci, and use LB medium at 37°C respectively Cultivate in a three-gas incubator for 20 hours; then separate the above strains and inoculate them into broth medium, and culture them in a constant temperature double-layer shaking incubator at 37°C for 28 hours, and the speed of the constant temperature shaking incubator is 300rpm. Then inactivate it for later use.

[0048] The six pathogenic bacteria after inactivation treatment were divided into 20 parts of Acinetobacter baumannii, 15 parts of Staphylococcus aureus, 15 parts of E. Mix 35 parts of streptococci to obtain a strain mixture.

[0049] Dissolve the mixed strain mixture in 0.2mol / L PBS solution to prepare the ...

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Abstract

The invention provides a specific yolk immunoglobulin composition and a preparation thereof, belonging to the field of medicines. The specific yolk immunoglobulin composition is prepared with six pathogenic bacteria, i.e., Acinetobacter baumannii, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Group-A Streptococcus, as antigens. The specific yolk immunoglobulin composition has good killing effect on Acinetobacter baumannii, solves the problem of pan-drug resistance of Acinetobacter baumannii and exerts better killing effect on the other five common concurrent pathogenic bacteria, so remarkable inhibition effect is performed on the growth of the pathogenic bacteria. The specific yolk immunoglobulin composition has good specificity, can specifically kill pathogenic bacteria, does not affect micro-ecological balance, is free of residues in human bodies and free of drug resistance and bad irritation, and has safety and broad application prospects.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a specific egg yolk immunoglobulin composition and its preparation. Background technique [0002] Acinetobacter baumannii has a very high resistance rate to most antibacterial drugs, and it is an important pathogen of nosocomial infection. One of the major public health issues of global concern. Pan-drug-resistant Acinetobacter baumannii has become a worldwide treatment problem. Recently, "fully drug-resistant" Acinetobacter baumannii appeared, which has attracted serious attention from clinical and microbiologists. It is difficult to choose antibacterial drugs based on experience, and often requires combination drugs, and requires large doses and long courses of treatment; clinically, the proportion of mixed infections is high, and common pathogenic bacteria combined include Staphylococcus aureus, Enterobacteriaceae, Pseudomonas aeruginosa, etc. Often need to cover other infectious bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40A61K39/395A61P31/04A61P31/10
CPCA61K39/395A61K39/40A61K2039/507A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 蒙永祥符美燕农定猛叶梦婕
Owner GUANGZHOU PHGO BIOTECH
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