Ordered collagen scaffold and applications of ordered collagen scaffold in preparation of spinal cord injury repair products
A collagen scaffold and spinal cord injury technology, applied in the field of biomedical materials, can solve problems such as unsatisfactory clinical effects, and achieve the effect of promoting growth and regeneration of spinal cord nerves
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[0051] Another aspect of the embodiments of the present invention provides a method for preparing an ordered collagen scaffold, which includes:
[0052] Dissolve the collagen raw material in 0.2~1mol / L acetic acid and let it stand overnight at 2~8℃, mix well and add 2~10mol / L lye to neutralize, and the obtained homogeneous liquid is put into the molecular weight cutoff of 3000~5000 In the dialysis bag, use deionized water for dialysis for 4-6 days. Change the deionized water every 3-6h during dialysis. Put the dialysis solution into a mold with a diameter of 4-10mm, and then make one end of the mold at -150~ -80℃, get ordered collagen sponge cylindrical scaffold;
[0053] The ordered collagen sponge cylindrical scaffold is dripped with liposomes encapsulating anticancer drugs with microtubule stabilizing effect, and then incubated at 25-37°C for 1 to 3 hours to obtain the ordered collagen scaffold.
[0054] Another aspect of the embodiments of the present invention also provides a f...
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