A kind of Seneca valley virus recombinant plasmid, recombinant virus and construction method

A technology for recombining plasmids and viruses, applied in the direction of viruses, viral peptides, viruses/phages, etc., can solve the problems of SVV infection characteristics, unclear host range, and no commercial vaccines

Active Publication Date: 2021-05-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many aspects of the infection characteristics, host range, and epidemiology of SVV are currently unknown, and there is no commercially available vaccine for prevention and control

Method used

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  • A kind of Seneca valley virus recombinant plasmid, recombinant virus and construction method
  • A kind of Seneca valley virus recombinant plasmid, recombinant virus and construction method
  • A kind of Seneca valley virus recombinant plasmid, recombinant virus and construction method

Examples

Experimental program
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Effect test

Embodiment 1

[0040](1) Building a routefigure 1 Indicated. According to the full-length genome sequence of SVV HB-CH-2016, four large segments were divided into four large segments: F1, F2, F3, F4 amplified its full length genome. At the same time, a CMV promoter sequence from PEGFP-N1 was simultaneously fused at the same time, and the 3 'end fusion-type hepatic viral ribozyme recognition sequence HDVR-POLY sequence was simultaneously. Each fragment was sequentially cloned to a low copy plaster PBLUESCRIPT II SK, and the infectious cloned plasmid of SVV HB-CH-2016 strain mediated by CMV promoter was obtained, named PSKII-CMV-SVV / Hb;

[0041](2) Savings of SVV infectious virus particles (RSVV)

[0042]figure 2 Save the RSVV, wherefigure 2 -A is a cytopathic chart of 18 hours for SVV HB-CH-2016 strain and RSVV infected with BHK-21 cells.figure 2 -B is the PCR cenmin of RSVV - PCR amplification for RSVV using SVV specific 5'UTR (366 bp), VP3 / 1 (542 bp) and 3d (298 bp) genes,figure 2 -C is a plaque fo...

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Abstract

The invention relates to a Seneca Valley virus recombinant plasmid, a recombinant virus and a construction method, belonging to the technical field of virus construction. The Seneca Valley recombinant virus provided by the present invention is: the 177th amino acid of the GH loop of capsid VP2 is mutated from S to A. The Seneca Valley recombinant virus provided by the invention has high virus titer and virus proliferation ability.

Description

Technical field[0001]The present invention relates to the field of viral construction techniques, and more particularly to a sessage, recombinant virus and construction method of seneca virus.Background technique[0002]Seneca Valley Virus (SVV), a small RNA virus, has a tumor-tumor cell, which can selectively infect neuroendocrine tumor cells, which in preclinical research and early clinical trials The prospect of cancer treatment is shown. It has been reported that the anthrax toxin receptor 1 (ANTXR1) is found to be a tumor endothelial cell marker 8 (TEM8) protein, which is the necessary receptor of the Sena Virus invading host cell. The interaction of SVV and ANTXR1 is directly and special, and this interaction requires SVV to bind to receptor cells, and to avoid antiviral activity of the interferon gene, the receptor cell needs high expression Antxr1, SVV nuclear casing direct recognition ANTXR1 receptor. At present, the infection characteristics of SVV, many aspects of hosting, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/85C12N15/66
CPCC07K14/005C12N7/00C12N15/85C12N2770/32021C12N2770/32022
Inventor 钱平李祥敏刘婷婷钱苏红陈焕春
Owner HUAZHONG AGRI UNIV
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