The invention relates to an avian
influenza vaccine and a preparation method thereof, in particular to the preparation of a recombinant
plasmid and a
recombinant virus in the avian
influenza vaccine preparation. The invention provides a recombinant
gene and a preparation method thereof, and the recombinant
gene is formed by fusing a baculovirus envelope
protein gp64
signal peptide gene sequence, a major
antigen gene HA sequence of an
avian influenza virus and a baculovirus envelope
protein gp64 transmembrane
gene sequence. The invention further provides a recombinant
plasmid that is constructed by recombining the recombinant gene and a pBacPAK8
plasmid, the recombinant
bombyx mori baculovirus BmHA that contains the recombinant gene and a
virus preparation method. The recombinant
bombyx mori baculovirus BmHA that contains the recombinant gene is preserved in China General
Microbiological Culture Center of Microbial Culture
Collection Management Committee with the preservation number of CGMCC No.2316. The invention further provides an
expression protein of the recombinant gene. The recombinant
bombyx mori baculovirus BmHA and the
protein that are provided by the invention can be used for preparing the avian
influenza vaccine. The methods of the invention are applicable to large-scale production, and have lowered cost, high yield and large application value of the produced avian influenza vaccine.