Method for producing gamma-aminobutyric acid through catalysis of saccharomyces cerevisiae cells and gamma-aminobutyric acid

A technology of Saccharomyces cerevisiae cells and aminobutyric acid, which is applied in the field of γ-aminobutyric acid, can solve the problems of incomparable GABA yield, low GABA synthesis efficiency, complex components, etc., and achieves a perfect exogenous gene expression system, easy control, and benefits. high rate effect
CN110106211AInactive Publication Date: 2019-08-09杭州唯铂莱生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
杭州唯铂莱生物科技有限公司
Publication Date
2019-08-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of biology, and aims at providing a method for producing gamma-aminobutyric acid through catalysis of saccharomyces cerevisiae cells. The method comprisesthe steps of 1, extracting a target gene from glutamic acid decarboxylase, conducting PCR amplification on the target gene, and connecting the amplified target genes to plasmids for recombination to construct a cloning vector; 2, conducting enzyme digestion treatment on the cloning vector through a restriction enzyme to obtain target gene fragments, and connecting the target gene fragments into acerevisiae vector to generate recombined plasmids; 3, guiding the recombined plasmids into the saccharomyces cerevisiae cells through a lithium acetate quick-conversion method to obtain recombined saccharomyces cerevisiae cells; 4, culturing the recombined saccharomyces cerevisiae cells in a Na2HPO4 citrate buffer solution, and mixing L-glutamic acid and 5'-pyridoxal phosphate for a reaction to obtain the gamma-aminobutyric acid. The invention further provides the gamma-aminobutyric acid. The production speed of the saccharomyces cerevisiae cells is high, culture is simple, an exogenous gene expression system is complete, and the method is high in catalysis efficiency, low in cost and easy to control.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for catalyzing the production of gamma-aminobutyric acid by brewer's yeast cells and gamma-aminobutyric acid. Background technique

[0002] γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid, white powder, widely present in nature, easily soluble in water, slightly soluble in hot ethanol, insoluble in cold ethanol, ether and benzene. GABA can reduce blood ammonia, promote brain metabolism, and regulate metabolic balance in the body; it can be used as an inhibitor of neurotransmitter signals, can regulate sleep, treat human headaches, hepatic coma, hypertension and other physiological diseases, and can also synthesize 2-pyrrolidone and 4-polyamide nylon, used in food and industrial production. Glutamate decarboxylase (GAD) is a 5'-pyridoxal phosphate (PLP)-dependent decarboxylase that exists in bacteria, yeast and filamentous fungi.

[0003] At present, the biolog...

Claims

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