A recombinant vector for assisting hydrogen production, a recombinant strain and its construction method and application

Abstract

The invention provides a recombinant vector pET32a-fhlA assisting hydrogen production, a recombinant strain and its construction method and application, belonging to the technical field of genetic engineering. The recombinant vector assisting hydrogen production includes an initial vector and an fhlA gene, and the initial The vector is pET32a vector; the recombinant strain includes the recombinant vector assisting hydrogen production. The recombinant strain can use sodium formate as a substrate to produce hydrogen, and when the final concentration of sodium formate is 60mmol / L, the amount of hydrogen produced is the highest, reaching 9.8mmol H 2 / L / h, the hydrogen production capacity is significantly higher than that of wild bacteria and empty plasmid recombinant bacteria, which can improve the hydrogen production efficiency.

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a recombinant carrier for assisting hydrogen production, a recombinant bacterial strain and a construction method and application thereof. Background technique

[0002] Biohydrogen production has attracted much attention due to its generation of clean and high-heat hydrogen energy. There are many microorganisms for biohydrogen production, among which Escherichia coli hydrogen production is one of the best choices in terms of cost and efficiency. At present, wild Escherichia coli does not produce hydrogen or produces little hydrogen, which cannot meet human needs for clean energy. Therefore, genetic engineering methods are used to transform Escherichia coli to greatly improve its hydrogen production performance. The existing hydrogen-producing Escherichia coli consumes a lot of substrates, but produces less hydrogen and has a relatively low efficiency. It ...

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