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Preparation method, printing method and application of bioprinting ink

A bio-printing and bio-printer technology, applied in inks, applications, household appliances, etc., can solve the problems of complicated printing process and long time, and achieve the effects of good biocompatibility, low price and easy preparation

Inactive Publication Date: 2018-12-21
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional 3D bioprinting method generally prints biocompatible polymer materials first to provide scaffolds, which is a complex and time-consuming printing process.

Method used

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  • Preparation method, printing method and application of bioprinting ink
  • Preparation method, printing method and application of bioprinting ink
  • Preparation method, printing method and application of bioprinting ink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Preparation of bioprinting ink

[0029] Weigh 12g of gelatin, 4.8g of sodium alginate, and 3g of hydroxyethyl cellulose, stir well in a square petri dish, and irradiate with ultraviolet light for 18 hours; In the bottle, heat it with magnetic stirring at 80°C for 2 hours; after it is completely dissolved, place it at room temperature for 2 hours, then place it in an oven at 80°C for 1 hour, and repeat this 3 times to obtain sterile bioprinting ink, as figure 1 shown.

Embodiment 2

[0030] Embodiment 2 Preparation of bioprinting ink

[0031] Weigh 4g of gelatin, 8g of sodium alginate, and 5g of hydroxyethyl cellulose, stir well in a square petri dish, and irradiate with ultraviolet rays for 18 hours; , heated with magnetic stirring at 80°C for 2 hours; after it was completely dissolved, it was placed at room temperature for 2 hours, and then placed in an oven at 80°C for 1 hour, and this was repeated 3 times to obtain sterile bioprinting ink.

Embodiment 3

[0032] Example 3 Preparation of bioprinting ink

[0033] Weigh 20 g of gelatin, 1 g of sodium alginate, and 0.5 g of hydroxyethyl cellulose, stir well in a square petri dish, and irradiate with ultraviolet rays for 18 hours; add the above-mentioned irradiated components and 78.5 mL of sterile saline In the bottle, heat it with magnetic stirring at 80°C for 2 hours; after it is completely dissolved, place it at room temperature for 2 hours, then put it in an oven at 80°C for 1 hour, and repeat this 3 times to obtain sterile bioprinting ink.

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PUM

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Abstract

The invention relates to the technical field of bio-ink, and discloses a preparation method, a printing method and application of bioprinting ink. The bioprinting ink comprises the following components in mass percent: 4%-20% of gelatin, 1%-8% of sodium alginate, 0.5%-5% of hydroxyethyl cellulose, and the balance of sterile saline. The invention provides the preparation method of the bioprinting ink, the bioprinting method of the bioprinting ink, and the application of the bioprinting ink in bioprinting. The bioprinting ink can be used for biological 3D printing after being mixed with cells. The used materials are inexpensive, are easy to prepare, have good biocompatibility, have good stability in a culture solution and support cell proliferation.

Description

technical field [0001] The invention relates to a preparation method of bioprinting ink, a printing method and its use, and belongs to the technical field of bioinks. Background technique [0002] In human tissues, cells are arranged and distributed according to certain rules and order, and different functional tissues have different cell arrangements. Therefore, realizing the precise arrangement of cells is the key to artificially constructing tissues and organs. 3D bioprinting technology is a technology that has emerged in recent years, and it has been tried to build complex tissues and organs. In 2010, Organovo launched a 3D bioprinter that can help users manufacture biological tissues for research and development. [0003] Bio-ink is an ink that can be used for 3D bio-printing. An ideal bio-ink requires a specific medium to ensure the survival of cells. It can provide energy for cells and ensure cell survival during the printing process, and it cannot clog the printer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D11/30
CPCC09D11/30
Inventor 罗勇李晓瑞高志刚林炳承
Owner DALIAN UNIV OF TECH
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