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Microcarrier for three-dimensional culture of cells as well as preparation method and application of microcarrier

A three-dimensional culture and microcarrier technology, applied in biochemical equipment and methods, tissue culture, microorganisms, etc., can solve the problems of poor cell adhesion, cumbersome processing steps, incomplete separation, etc., and achieve biocompatibility and cell adhesion. Good adhesion, cost reduction, and avoidance of high cell seeding density requirements

Pending Publication Date: 2022-04-12
上海食未生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of edible, dissolvable microcarrier for cell three-dimensional culture and its preparation method and application, be used for the quick, mass production of cell, to solve There are many problems in the prior art, such as poor cell adhesion of microcarriers, or because microcarriers are inedible, microcarriers need to be separated, resulting in cumbersome processing steps or incomplete separation, etc. Provide support for streamlined production and reduced costs

Method used

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  • Microcarrier for three-dimensional culture of cells as well as preparation method and application of microcarrier
  • Microcarrier for three-dimensional culture of cells as well as preparation method and application of microcarrier
  • Microcarrier for three-dimensional culture of cells as well as preparation method and application of microcarrier

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preparation example Construction

[0049] The invention provides a microcarrier for three-dimensional cell culture and a preparation method thereof, the method comprising: directly mixing edible plant-derived ion-crosslinked gel-type polysaccharides and edible non-animal-derived proteins , make a mixed liquid, generate liquid droplets, and prepare microcarriers through appropriate cross-linking and immobilization reaction conditions. The microcarrier is used for cell culture, can support the rapid proliferation of cells, and through the interaction between cells, spontaneously aggregate to form cell-microcarrier clumps. According to the needs of the actual product form, the cells can be harvested by dissolving the microcarriers for subsequent processing, or the cell culture meat products can be obtained directly by bonding and extruding the agglomerates. The microcarrier prepared by the method of the invention is used in the cell culture meat, and the operation is fast and simple, and it is easy to realize larg...

Embodiment 1

[0108] Embodiment 1: Preparation of soybean protein isolate-sodium alginate / polylysine surface modified microcarrier

[0109] Weigh 1 g of sodium alginate and add it into 100 ml of deionized water, stir at room temperature until completely dissolved, and prepare a sodium alginate solution with a concentration of 1%. Weigh 0.1 g of edible soda ash and add it to 100 ml of deionized water, stir to dissolve, then weigh 5 g of soybean protein isolate solid, add it to the alkali solution, stir at room temperature until completely dissolved, and prepare a soybean protein isolate solution with a concentration of 5%. Measure 20ml concentration of 1% sodium alginate solution, measure 80ml concentration mass volume percent concentration (g / mL) of 5% soybean protein isolate solution, mix the two solutions uniformly, and prepare sodium alginate concentration of 0.2% and 4% soy protein isolate compound solution, the mass ratio of soy protein isolate to sodium alginate is 20:1. Micro-drople...

Embodiment 2

[0110] Embodiment 2: the preparation of yeast protein-low ester pectin microcarrier

[0111] Weigh 2 g of low-ester pectin and add it into 100 ml of deionized water, heat in a water bath at 60° C. and stir until completely dissolved, and prepare a low-ester pectin solution with a concentration of 2%. Weigh 5g of solid yeast protein and add it to 100ml of 2% low-ester pectin solution, stir to disperse the solid particles evenly, and the mass ratio of yeast protein to low-ester pectin is 2.5:1. Weigh 25 g of calcium chloride solid and add it into 500 ml of deionized water to form a 5% calcium chloride solution, which is used as a collection bath. Micro-droplets were prepared by electrostatic spraying, and the generated micro-droplets were cross-linked and fixed in a collection bath at room temperature for 30 minutes to obtain ion-crosslinked polysaccharide-protein composite microcarriers with a particle size of 200-800 μm.

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Abstract

The invention belongs to the technical field of biological materials and future food, and discloses an edible and dissoluble microcarrier for three-dimensional cell culture as well as a preparation method and application of the edible and dissoluble microcarrier. The method comprises the following steps: directly mixing edible plant-derived ionic cross-linked gel type polysaccharide and edible non-animal-derived protein to prepare a mixed solution, then preparing the mixed solution into liquid drops, and carrying out a cross-linking reaction to obtain the microcarrier. The invention also discloses a modified microcarrier obtained by carrying out surface modification on the microcarrier. The microcarrier is edible, soluble, good in biocompatibility and cell adhesion, capable of supporting adhesion, growth and proliferation of cells, capable of being used for rapidly producing cells in a large scale and reducing the cost of cell culture meat products, especially used in the field of cell culture meat, and wide in application prospect.

Description

technical field [0001] The invention relates to a microcarrier for three-dimensional cell culture and its preparation method and application, in particular to an edible and dissolvable microcarrier for cell three-dimensional culture and its preparation method and application, in particular to The application of the invention in spontaneous aggregation to form micro-agglomerates belongs to the field of biomaterials and future food technology. Background technique [0002] Traditional animal husbandry produces a large amount of greenhouse gases and causes soil and water pollution. In addition, the production and consumption of animal meat products may lead to the spread of pathogens and endanger public health. Compared with traditional cultured meat, cell cultured meat is a new type of biotechnology based on the principle of tissue engineering, which simulates the process of cell growth and differentiation in a clean environment in vitro, and cultivates animal muscle tissue f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C08L89/00C08L5/04C08L5/00C08L5/06
Inventor 刘雨灵向宁
Owner 上海食未生物科技有限公司
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