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Quick detecting method of skeletonema pseudocostatum, and preparation and using methods of test strip

A technology of test strips and sclerophytes, which is applied in the field of immunochromatographic detection of planktonic algae, can solve the problems of no papers and patent reports, etc., and achieve the effect of fast detection and simple operation

Inactive Publication Date: 2018-12-25
XIAMEN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the labeled antibody also requires the same diffusion binding process to form an antibody-antigen-labeled antibody complex, so it takes a long time
No papers and patent reports similar to this project have been found so far

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  • Quick detecting method of skeletonema pseudocostatum, and preparation and using methods of test strip
  • Quick detecting method of skeletonema pseudocostatum, and preparation and using methods of test strip
  • Quick detecting method of skeletonema pseudocostatum, and preparation and using methods of test strip

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preparation example Construction

[0061] Two, the concrete steps of the preparation method of the immunochromatographic detection and analysis test paper strip of described Skeletalum pseudosclerosteosa are as follows:

[0062] Through the sample test of known concentration of Skeletalum skeletalum, the concentration of the capture antigen required for detection of the algae cell sample is determined, and the detection line is drawn on the membrane to obtain the immunochromatographic detection and analysis test strip of Sclerosanthra sclerotinum.

[0063] Take out 80-100 μL of the sample solution to be tested, and directly drop it on the sample loading place of the immunochromatographic detection and analysis test strip of Skeletalum costarum, observe the results after 3 minutes at the fastest and an average of about 15 minutes. After reacting with the antibody, the difference in color displayed on the immunochromatographic detection and analysis test strip of Skeletodera costatum was established to establish t...

Embodiment 1

[0066] Example 1: Preparation of Immunogen

[0067] The vigorously growing Skeletalum costatum cell culture solution was fixed overnight with formaldehyde at a final concentration of 2%, centrifuged at 10,000 r / min for 10 min, and fine algae precipitates were collected. The algal cell aggregates were washed once with distilled water and twice with PB 5, and the algal cells were collected by centrifugation each time, and the centrifugation conditions were 10000r / min, 10min. The collected algae cells were divided into 1.5ml Eppendorf tubes, the residual water was shaken off, and stored at -20°C for later use. Take it out just before use, and resuspend it evenly with 5ml of sterile PBS. Obtain a standard solution of algae cells of known cell density. A certain amount of algae cell suspension is mixed with an equal volume of Freund's complete adjuvant and emulsified. The degree of emulsification is as complete as possible, and finally the immune antigen for animal injection is p...

Embodiment 2

[0068] Example 2: Preparation of monoclonal antibodies. See References (Xu Zhikai. Practical Monoclonal Antibody Technology [M]. Xi'an: Shaanxi Science and Technology Press, 1992: 27-36)

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Abstract

The invention provides a quick detecting method of skeletonema pseudocostatum, and preparation and using methods of a test strip, relates to floating algae immunochromatographic assay detecting basedon a gold immune chromatography assay (GICA) analyzing technology, and provides an immunochromatographic assay test strip of skeletonema pseudocostatum, a preparation method of the immunochromatographic assay test strip of the skeletonema pseudocostatum, a using method of the immunochromatographic assay test strip of the skeletonema pseudocostatum, and an immunochromatographic assay detecting method of the skeletonema pseudocostatum. Through an immunology technique, a monoclonal antibody of floating algae is prepared through algal cell immune experimental animals, and an immunoassay method ofthe GICA is established by utilizing the antibody; through a shade guide, the chromogenic results of the GICA are compared, and thus the concentration of hab algae in a water body is estimated; and awater sample containing the algae is directly dropped on the test strip, thus the positive result can be obtained, and the approximate numerical results of the algal cell concentration can be given according to the strength of a reaction.

Description

technical field [0001] The present invention relates to the immunochromatographic detection of planktonic algae based on colloidal gold immunochromatographic analysis technology (gold immune chromatographya55ay, GICA), especially relates to the field of immunochromatographic detection and analysis test strips of Skeletalum costalis and its preparation and detection methods and The invention relates to the field of monitoring the cell concentration of Mesosostrea costatum, which belongs to the cross technical field of immunology and plankton biology. Background technique [0002] The classification, identification and quantification of phytoplankton have always been one of the most basic topics in phycology and ecology research, and are of great significance to ecological dynamics, red tide occurrence mechanism and early warning and forecast, and routine detection of mariculture waters. For the application fields such as routine environmental monitoring and fish farming water...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/577G01N33/558
Inventor 周克夫孟坤张长弓李明洋陈闯王心怡王英子
Owner XIAMEN UNIV
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