Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method of producing kidney bean or lentil mutants using ems mutagenesis

A technology of lentils and mutants, applied in the directions of horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as the inability to simulate the influence of environmental factors, and achieve the effect of improving the survival rate

Active Publication Date: 2021-11-09
CORN RES INST SHANXI ACAD OF AGRI SCI
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, relying solely on the chemical mutagenic factors of the EMS mutagen to mutate kidney beans and lentils cannot simulate the influence of environmental factors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of producing kidney bean or lentil mutants using ems mutagenesis
  • A method of producing kidney bean or lentil mutants using ems mutagenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0041] S1, the plant seeds were soaked in water for 12 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were kidney bean seeds; the specific steps were as follows:

[0042] Select red kidney bean seeds with consistent color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml of HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0043] S2, germination treatment

[0044]Place the sterile seeds flat on the germination matrix, and make the liquid level of the germination matrix 2 mm lower than the upper surface of the sterile seeds, put them in an artificial climate box to germinate the sterile seeds, and after one young leaf gro...

Embodiment 2

[0052] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0053] S1, the plant seeds were soaked in water for 6 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were kidney bean seeds; the specific steps were as follows:

[0054] Select red kidney bean seeds with consistent color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml of HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0055] S2, germination treatment

[0056] Place the sterile seeds flat on the germination matrix, and make the liquid level of the germination matrix 1 mm lower than the upper surface of the sterile seeds, put them into an artificial climate box to germinate the sterile seeds, and after two young leaves...

Embodiment 3

[0062] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0063] S1, the plant seeds were soaked in water for 12 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were lentil seeds; the specific steps were as follows:

[0064] Select the lentil seeds with the same color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0065] S2, germination treatment

[0066] Place the aseptic seeds flat in the germination matrix, and make the liquid level of the germination matrix lower than the upper surface of the aseptic seeds, put them into an artificial climate box to germinate the aseptic seeds, and after two young leaves grow, the Germina...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of kidney bean breeding, and in particular relates to a method for producing kidney bean or lentil mutants through EMS mutagenesis. It includes seed disinfection treatment, germination treatment, mutagenesis treatment and field planting, wherein the germination treatment step is: placing the sterile seeds in a germination matrix, and making the germination matrix liquid level lower than the upper surface of the sterile seeds, so that the sterile seeds The fungus seeds germinate, and after 1 to 2 young leaves grow, the germinated plant is obtained; the mutagenesis treatment step is: add EMS solution to each kilogram of germination substrate, use the EMS solution to mutate the germinated plant, and then take out the germinated plant , placed in MS liquid medium for photoperiod culture for 5 to 7 days, then adding EMS solution to each kilogram of MS liquid medium, and using EMS to continue mutagenizing sprouting plants to obtain mutagenized plants M0. The method of the present invention is a special high-efficiency mutagenesis method for red kidney beans, which improves the mutagenesis efficiency, reduces the consumption of carcinogens such as EMS, and reduces the health threat to breeders.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for producing kidney bean or lentil mutants through EMS mutagenesis. Background technique [0002] Mutagenesis is one of the more commonly used methods in plant breeding, including (1) physical mutagenesis, such as radiation, laser microbeam, ion beam, microwave, ultrasonic, heat and other methods to cause heritable variation in plants. It mainly acts on the DNA in plants through these ion beams, causing DNA fragment loss, translocation, inversion, etc.; (2) chemical mutagenesis, which uses chemical mutagens to treat plant materials. There are many types of chemical mutagens, such as alkylating agents, nitroso compounds, azide compounds, base analogs, antibiotics, and light amines. At present, among these chemical mutagens, the most widely researched and applied ones are mainly alkylating agents, mainly including, EMS, NaN3, DES and methyl sub-MNU. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H3/02A01H3/04A01H4/00
CPCA01H3/02A01H3/04A01H4/001
Inventor 郭贵青陈喜明韩云丽
Owner CORN RES INST SHANXI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com