Method for preparing D-amino acid and alpha keto acid

A technology of alpha keto acid and amino acid, applied in the field of biology

Inactive Publication Date: 2018-12-28
浙江正硕生物科技有限公司
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are other methods th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing D-amino acid and alpha keto acid
  • Method for preparing D-amino acid and alpha keto acid
  • Method for preparing D-amino acid and alpha keto acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] A preparation method of D-amino acid and alpha keto acid, comprising the steps of:

[0038] 1. Construction of recombinant bacteria:

[0039] Total gene synthesis from Proteus mirabilis ( Proteus mirabilis) L-amino acid deaminase (L-aminoacid deaminase, LAAD) gene (genbank accession number EU669819.1) LAAD, and connected into the pET24a vector ( NdeI / Hind ), construct the plasmid LAAD, transform it into E.Coli BL21 (DE3), and construct the recombinant strain PMLAAD.

[0040] Total gene synthesis is derived from lactic acid bacteria ( Lactobacillus buchneri strain JCM 1115) amino acid racemase (amino acid racemase, AAR) gene (genbank accession number KC413940.1) AAR, and connected into the pET24a vector ( NdeI / Hind ), construct the plasmid AAR, transform it into E.Coli BL21 (DE3), and construct the recombinant strain AAR.

[0041] 2. Fermentation

[0042] Recombinant bacteria PMLAAD and AAR were fermented with TB.

[0043] 3) Medium

[0044] (1) LB:

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of biotechnology, and relates to a method for preparing D-amino acid and alpha keto acid. The method includes the first step of recombinant construction: conductingwhole gene synthesis to obtain an L-amino acid deaminase gene LAAD and connecting an expression vector to be transformed into an expression strain to construct a recombinant PMLAAD; conducting whole gene synthesis to obtain an amino acid racemase gene AAR and connecting the expression vector to be transformed into the expression strain to construct a recombinant AAR; the second step of fermentation; the third step of enzyme catalysis; the fourth step of isolation and purification. The alpha-keto acid calcium and the D-amino acid are produced by enzyme catalysis, and both products have economicvalue and high product quality, so that the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of D-amino acid and alpha keto acid. Background technique [0002] There are 20 kinds of amino acids in nature, all of which are L-type amino acids, which are the basic structural units of proteins. Therefore, it is generally believed that D-amino acids rarely exist in nature. In recent years, with the deepening of scientific research, it has been found that although D-amino acids are not the basic structural nitrogen source of proteins, they are found in many plants, microorganisms and higher plants. Presence of D-amino acids. [0003] At present, the application fields of D-amino acid are mainly: 1. Medicine. For the synthesis of peptide drugs, such as peptide antibiotics (Apoxicillin ASPOXICILLIN), gastrointestinal drugs (OXTREOTIDE), diuretics, diarrhea drugs (SANDOSTATINACETATE), enzyme inhibitors, corticotropin analogs, analgesic drugs, weight loss Drugs, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P13/08C12P13/06C12P13/22C12P7/40C12N1/21C12R1/19
CPCC12N9/0022C12N9/90C12P7/40C12P13/06C12P13/08C12P13/222C12Y104/03002C12Y501/0101
Inventor 吴黎诚郭小雷章权
Owner 浙江正硕生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap