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Manufacturing method of flexible zirconia nanofiber phosphorylated peptide enrichment material

A technology of nanofibers and phosphorylated peptides, which is applied in the direction of heating/cooling fabrics, textiles and papermaking, and fabric surface trimming, etc. It can solve the problems of complex preparation process and poor fiber strength, and achieve good continuity, large specific surface area, and high efficiency. The effect of effective enrichment and purification

Inactive Publication Date: 2019-01-01
XI'AN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a flexible zirconia nanofiber phosphorylated peptide-enriched material, which solves the problem that polymers need to be added in the preparation process of the prior art, the preparation process is complicated, and the strength of the obtained fiber is poor The problem

Method used

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  • Manufacturing method of flexible zirconia nanofiber phosphorylated peptide enrichment material
  • Manufacturing method of flexible zirconia nanofiber phosphorylated peptide enrichment material
  • Manufacturing method of flexible zirconia nanofiber phosphorylated peptide enrichment material

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Step 1: Dissolve zirconyl nitrate, zirconium acetate and tetrabutyl titanate in the mixed solvent of ethanol / water / acetic acid in sequence, first stir for 10 minutes, then add chelating agent acetylacetone, and continue stirring for 20 minutes, wherein zirconium oxynitrate and acetic acid The mass ratio of zirconium is 1:1, the molar ratio of zirconium oxynitrate, zirconium acetate and tetrabutyl titanate is 100:0.8, the ratio of zirconium oxynitrate, zirconium acetate and ethanol / acetic acid is 1g:40ml, ethanol / water / acetic acid The volume ratio of zirconium oxynitrate and chelating agent acetylacetone is 1:1:1, and the molar ratio of zirconyl nitrate and chelating agent acetylacetone is 1:0.3; mix uniformly to make a uniform and stable precursor solution with a dynamic viscosity of 5Pa s, and the molecular chain in the precursor solution It has a three-dimensional stable polycyclic structure formed by acetylacetone ligands and central metal zirconium ions, and its stru...

Embodiment 2

[0057] Step 1: Dissolve zirconium hydroxide and tin oxalate successively in the mixed solvent of acetic acid / hydrochloric acid, add chelating agent nitrosalicylic acid after first stirring 50min, continue to stir again 20min, wherein the mol ratio of zirconium hydroxide and tin oxalate is 100:0.05, the ratio of zirconium hydroxide to acetic acid / hydrochloric acid is 1g:20ml, the volume ratio of acetic acid to hydrochloric acid is 1:2, the molar ratio of zirconium hydroxide to chelating agent nitrosalicylic acid is 1:0.4; mix well A uniform and stable dynamic viscosity is prepared as a precursor solution of 0.2Pa·s, and the molecular chain in the precursor solution has a three-dimensional stable polycyclic structure similar to that of Example 1;

[0058] Table 2 Commercial TiO 2 Phosphorylated peptides detected in β-casein digests

[0059]

[0060] Step 2: The above-mentioned precursor solution is made into a precursor nanofiber through an electrospinning forming process. ...

Embodiment 3

[0063] Step 1: Dissolve zirconium acetate and ferrous sulfide in the mixed solvent of ethanol / acetic acid in sequence, first stir for 130 minutes, then add the chelating agent diethylenetriamine-pentamethylene phosphonic acid, and continue stirring for 60 minutes, in which the zirconium acetate The molar ratio to ferrous sulfide is 100:10, the ratio of zirconium acetate to ethanol / acetic acid is 1g:50ml, the volume ratio of ethanol to acetic acid is 1:1, zirconium acetate and chelating agent diethylenetriamine-pentamethylene The molar ratio of phosphonic acid is 1:0.2; uniformly mixed to make a uniform and stable dynamic viscosity of 0.05Pa s precursor solution, the molecular chain in the precursor solution has a three-dimensional stable polycyclic structure similar to that of Example 1;

[0064] Step 2: The above-mentioned precursor solution is made into a precursor nanofiber through an electrospinning forming process. Electrospinning process parameters: spinning temperature ...

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Abstract

The invention discloses the manufacturing method of a flexible zirconia nanofiber phosphorylated peptide enrichment material. The method comprises the following steps of 1) sequentially dissolving zirconium salt and non-zirconium metal salt in a solvent, stirring for 10 to 130 minutes, then adding a chelating agent, and continuously stirring for 20 to 60 minutes to obtain a precursor solution, wherein the molar ratio of the zirconium salt to the non-zirconium metal salt is 100:0.05-30; the ratio of the zirconium salt to the solvent is 1g: 20-80mL; and the molar ratio of the zirconium salt to the chelating agent is 1:0.1-0.4; 2) carrying out electrostatic spinning on the precursor solution to obtain a precursor nanofiber film; and 3) calcining the precursor fiber material at a high temperature in air to obtain a flexible zirconia nanofiber film. The method has simple steps, the manufactured flexible zirconia nanofiber has a flexible and large specific surface area, and a phosphorylatedprotein or phosphorylated peptide segment can be repeatedly and high-efficiently enriched and purified.

Description

technical field [0001] The invention belongs to the technical field of new materials, and relates to a preparation method of a flexible zirconia nanofiber phosphorylated peptide enrichment material. Background technique [0002] Protein phosphorylation is a common and important means of protein modification and translation. Protein phosphorylation regulates the process of the entire life activity, but the low abundance of phosphorylated peptides in protein hydrolysis products and the high concentration of non-phosphorylated peptides Abundance significantly reduces the response intensity of mass spectrometry to phosphorylated peptides, and even cannot detect phosphorylated peptides, so it is necessary to enrich and purify phosphorylated peptides. Currently commonly used phosphorylation enrichment methods include strong cation exchange method, immobilized metal ion exchange method and lectin affinity method, etc. These methods have the problems of complicated operation and uns...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728D06C7/04
CPCD04H1/4382D04H1/728D06C7/04
Inventor 毛雪阳智刘呈坤吴红韩伟东金菁孙润军
Owner XI'AN POLYTECHNIC UNIVERSITY
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