Long-acting hypoglycemic and lipid-regulating polypeptide and application thereof
A blood sugar-lowering and fat-lowering technology, applied in the field of biochemical pharmacy, can solve problems such as lack of treatment methods, and achieve the effects of reducing glycated hemoglobin, lowering blood sugar, and inhibiting immune attack
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Embodiment 1
[0029] Embodiment 1 The duration of drug effect of polypeptide of the present invention and Progly peptide, VP peptide is compared
[0030] (1) Acute and sustained hypoglycemic effect of Progly peptide on normal mice
[0031] 8-week-old male C57BL / 6 mice were randomly divided into 5 groups, 10 in each group, respectively solvent control group (negative control group), PGLP-1 group (1nmol / kg), PGLP-1 group (30nmol / kg kg), PGLP-1 group (100nmol / kg), GLP-1 group (30nmol / kg) for glucose tolerance test. Wherein, PGLP-1 refers to Progly peptide, and GLP-1 refers to glucagon-like peptide-1.
[0032] The result is as figure 1 As shown in A, compared with the control group, both GLP-1 and PGLP-1 can significantly reduce the blood sugar level, and the area under the curve is significantly lower than that of the control group, indicating that PGLP-1 can be as sugar-dependent as GLP-1 Insulin-stimulating effect. At the same time, PGLP-1 (1, 30, 100nmol / kg) can lower blood sugar in a d...
Embodiment 2
[0041] Embodiment 2 Pharmacodynamic evaluation of the polypeptide of the present invention
[0042] (1) Modeling and grouping of STZ diabetic model mice
[0043] Eighty 6-week-old male c57 mice (purchased from the Experimental and Comparative Medicine Center of Yangzhou University, license number: SCXK (Su) 2012-0004) were adaptively fed for 1 week. Fasting for 12 hours before the experiment, intraperitoneal injection of STZ solution (citrate buffer solution with pH = 5.2) at a dose of 50 mg / kg for 5 consecutive days, fasting blood glucose was detected 1 week and 2 weeks after injection, and the blood glucose values were all over 11.0mmol / L means successful modeling.
[0044] The model mice were randomly divided into 4 groups, 12 in each group, which were solvent control group (negative control group), PGLP-1 group (positive control group), VP group (positive control group), and P15 group (experimental group). The dosage is 30nmol / kg, twice a day, the solvent is normal sa...
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