Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA barcode standard detection fragment COII and kit to identify Periplaneta americana and application method of kit

A standard detection technology for Periplaneta americana, applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., to achieve strong specificity and high sensitivity

Inactive Publication Date: 2019-01-04
DALI UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective DNA barcode identification method with high accuracy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA barcode standard detection fragment COII and kit to identify Periplaneta americana and application method of kit
  • DNA barcode standard detection fragment COII and kit to identify Periplaneta americana and application method of kit
  • DNA barcode standard detection fragment COII and kit to identify Periplaneta americana and application method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Access to DNA Barcoding Standard Genes for Periplaneta americana

[0066] 1. Collection and storage of samples to be tested

[0067] The adults of Periplaneta americana raised in the standardized breeding base of the national and local joint engineering research center for the development of special medicinal insects (Yunnan Province) were identified as Periplaneta americana by Professor Yang Zizhong of Dali University according to the morphological method. Linn.). After the identification was correct, part of the muscle tissue was extracted from fresh specimens and stored in a -20°C freezer.

[0068] 2. Preparation of experimental instruments and reagents

[0069] 2.1 Experimental instruments and reagents

[0070] HWS12 constant temperature electric heating constant temperature water bath (Shanghai Yiheng Technology Co., Ltd., serial number 131022035);

[0071] SIGMA 1-14ED centrifuge (Sigma, Germany, serial number 133828);

[0072] DYCP-31DN electrophoresis instr...

Embodiment 2

[0136] Embodiment 2 is used to distinguish the kit of American cockroach

[0137] Positive control: adults of Periplaneta americana;

[0138] Primer pair: forward primer and reverse primer synthesized in embodiment 1, wherein the concentration of forward primer and reverse primer is 10 μ mol / L;

[0139] PCR reaction mixture: 0.1U Taq polymerase, 500μmol / L dNTP, 20mmol / L Tris-HCl with pH=8.3, 100mmol / L potassium chloride and 3μmol / L magnesium chloride;

[0140] 38 parts of distilled water.

Embodiment 3

[0142] Identification of samples of different forms and processed products of Periplaneta americana

[0143] 1. Collection and storage of the samples to be tested: Periplaneta americana, which was raised in batches at the Zuoyi Base for Standardized Breeding of Medicinal Insects under the National and Local Joint Engineering Research Center for the Development of Special Medicinal Insects located in Dali Bai Autonomous Prefecture, Yunnan Province, was taken as the samples to be tested. include:

[0144] The 1-day-old larvae, larvae, and adults that had just come out of their egg sheaths, as well as the processed imagoes that had been quickly dried in an oven, and adults purchased from the medicinal material market and identified as Periplaneta americana by Professor Yang Zizhong of Dali University were positive control samples.

[0145] Periplaneta americana has been reviewed by authoritative experts before mass breeding. Part of the muscle tissue of the positive control samp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of species identification and provides a kit to identify Periplaneta americana. The kit includes a positive control, forward and reverse primers, a PCR (polymerase chain reaction) mixed liquid and distilled water. The positive control is DNA barcode standard detection fragment COII shown as SEQ ID NO. 1; the forward and reverse primers have sequences shown as inSEQ ID NO. 2 and SEQ ID NO. 3. In the invention, genetic fragment COII (SEQ ID NO. 1) from Periplaneta americana has homology of 98-100% in the species of Periplaneta americana, and has significantlyreduced gene homology against non-Periplaneta americana species; primer design and PCR amplification help accurately distinguish Periplaneta americana and other similar species. The kit provided herein is suitable for identifying powdery or processed Periplaneta americana, and has a wider detection range, higher detection accuracy and short identifying time.

Description

technical field [0001] The invention relates to the technical field of species identification, in particular to a kit for identifying Periplaneta americana and a use method thereof. Background technique [0002] The American cockroach (Periplaneta americana Linn.) is a long-term used animal medicine in the folk. It is often used as dried or fresh adults as medicine. In recent years, studies have shown that the clinical application records of Periplaneta americana as an insect drug have shown that it has pharmacological effects such as anti-tumor, treatment of cardiovascular diseases, promotion of tissue repair, antibacterial, anti-virus, anti-oxidation, and immune enhancement. Pharmaceutical preparations of Periplaneta americana It is also more and more widely used clinically, and the authenticity identification of Periplaneta americana medicinal materials is the basis for medicinal use and pharmacological research. [0003] At present, the species identification of insects...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2563/185
Inventor 耿福能巫秀美刘彬陈壮志沈咏梅马秀英吴桃清白丽
Owner DALI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com