DNA barcode standard detection fragment COII and kit to identify Periplaneta americana and application method of kit

A standard detection technology for Periplaneta americana, applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., to achieve strong specificity and high sensitivity
CN109136389AInactive Publication Date: 2019-01-04DALI UNIV
3 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALI UNIV
Publication Date
2019-01-04
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the field of species identification and provides a kit to identify Periplaneta americana. The kit includes a positive control, forward and reverse primers, a PCR (polymerase chain reaction) mixed liquid and distilled water. The positive control is DNA barcode standard detection fragment COII shown as SEQ ID NO. 1; the forward and reverse primers have sequences shown as inSEQ ID NO. 2 and SEQ ID NO. 3. In the invention, genetic fragment COII (SEQ ID NO. 1) from Periplaneta americana has homology of 98-100% in the species of Periplaneta americana, and has significantlyreduced gene homology against non-Periplaneta americana species; primer design and PCR amplification help accurately distinguish Periplaneta americana and other similar species. The kit provided herein is suitable for identifying powdery or processed Periplaneta americana, and has a wider detection range, higher detection accuracy and short identifying time.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of species identification, in particular to a kit for identifying Periplaneta americana and a use method thereof. Background technique

[0002] The American cockroach (Periplaneta americana Linn.) is a long-term used animal medicine in the folk. It is often used as dried or fresh adults as medicine. In recent years, studies have shown that the clinical application records of Periplaneta americana as an insect drug have shown that it has pharmacological effects such as anti-tumor, treatment of cardiovascular diseases, promotion of tissue repair, antibacterial, anti-virus, anti-oxidation, and immune enhancement. Pharmaceutical preparations of Periplaneta americana It is also more and more widely used clinically, and the authenticity identification of Periplaneta americana medicinal materials is the basis for medicinal use and pharmacological research.

[0003] At present, the species identification of insects...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More