Three-dimensional thin film structure having microparticles enclosed therein and method for manufacturing same
A technology of micro-particles and manufacturing methods, which can be used in methods of supporting/immobilizing microorganisms, biochemical equipment and methods, and culturing devices for tissue cells/viruses, etc. problem, to achieve the effect of high import efficiency
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Embodiment 1
[0119] [Example 1] Fabrication of a thin film capable of self-assembly into a three-dimensional structure
[0120] Fabrication of thin films capable of self-assembling into three-dimensional structures according to Figure 5A~5F The process shown was carried out. In this embodiment, the substrate 13 is made of a glass substrate, and the sacrificial layer 12 is made of calcium alginate gel. First, a sodium alginate solution was spin-coated on the substrate 13 which is a glass substrate. Thereafter, the spin-coated substrate 13 was immersed in a 100 mM calcium chloride solution, thereby forming a sacrificial layer 12 formed of a physical gel of calcium alginate ( Figure 5A ). The thickness of calcium alginate gel can be controlled by making the concentration of sodium alginate solution and the speed of spin coating, in the present embodiment, by spin coating 2 wt% sodium alginate solution with 3000rpm, thus form the gel of 40nm layer.
[0121] Next, the thin film layer 10 ...
Embodiment 2
[0126] [Example 2] Self-assembly of a three-dimensional structure based on a thin film
[0127] Self-assembly of three-dimensional structures containing cells in accordance with Figure 5G~5J The process shown was carried out. The substrate 13 adhered to the film 1 made in Example 1 and the sacrificial layer 12 was immersed in the protein solution, and the surface of the parylene film of the film layer 11 was modified by protein ( Figure 5G ). The type of protein modification is appropriately selected according to the type of cells to be encapsulated. In this example, the thin film layer 11 was modified using a 1 mg / mL fibronectin solution in order to induce the adhesion of strained cultured cells. A 1 mg / mL fibronectin solution was simultaneously added to the culture medium when inoculating the strained cultured cells, and was prepared so that the final concentration would be 1 μg / mL. Furthermore, in order to induce adhesion of primary nerve cells, modification of the th...
Embodiment 3
[0133] [Example 3] Culture of Adhesive Cells Included in a Cylindrical Structure
[0134] In this example, Chinese hamster-derived ovum (CHO) cells and human embryo-derived kidney (HEK) cells were used as strained cultured cells as the cells enclosed in the cylindrical structure. Both cells were cultured using Duchenne's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) as a culture medium. The two types of cells were cultured in a humid environment maintaining a temperature of 37° C. and a carbon dioxide concentration of 5%.
[0135] The fabrication of the cylindrical structure and the encapsulation of cells were carried out as described in Examples 1 and 2. One week after being encapsulated in the cylindrical structure, the life and death of the cells were judged, and it was confirmed that both CHO cells and HEK cells survived in the cylindrical structure. In addition, in CHO cells and HEK cells, which are strain-cultured cells that proliferate indefin...
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