Detection of arsenic and cadmium mixed exposure as low birth weight auxiliary diagnosis marker based on ICP-MS (inductively coupled plasma-mass spectrometry) and application of marker

An ICP-MS, low birth weight technology, applied in the field of analytical chemistry and clinical medicine, can solve the problems that have not received corresponding attention, and achieve the effects of delaying and preventing disease progression, improving sensitivity, and high practical value in clinical diagnosis

Inactive Publication Date: 2019-01-08
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of ICP-MS to analyze the mixed exposure level of arsenic and cadmium in whole blood during pregnancy has not received corresponding attention in the auxiliary diagnosis of low birth weight.

Method used

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  • Detection of arsenic and cadmium mixed exposure as low birth weight auxiliary diagnosis marker based on ICP-MS (inductively coupled plasma-mass spectrometry) and application of marker
  • Detection of arsenic and cadmium mixed exposure as low birth weight auxiliary diagnosis marker based on ICP-MS (inductively coupled plasma-mass spectrometry) and application of marker
  • Detection of arsenic and cadmium mixed exposure as low birth weight auxiliary diagnosis marker based on ICP-MS (inductively coupled plasma-mass spectrometry) and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1 Research Object Selection and Grouping Basis

[0080] The inventor collected low birth weight cases that met the requirements from the Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University

[0081] and whole blood samples from normal control pregnant women. Randomly enrolled 100 mothers of low birth weight infants (weight <2500g)

[0082] Humans, according to age, BMI, infant sex matching normal weight infants (2500g≤weight<4000g)

[0083] 100 mothers, a total of 200 people were used as the experimental subjects for the mixed exposure assessment of arsenic and cadmium with low birth weight. specific

[0084] Sample classification criteria are as follows:

[0085] The first stage of association exploration

[0086] Objects: 100 mothers of low birth weight infants were included, and the positive control group was matched according to age, BMI, and infant sex.

[0087] 100 mothers of infants with normal weight (2500g≤weight<...

Embodiment 2

[0095] Example 2 Analysis of the relationship between arsenic and cadmium mixed exposure and low birth weight detected by ICP-MS

[0096] 1. Sample pretreatment

[0097] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix well.

[0098] 2. Instrument testing

[0099] Analytical instrument: ICAP RQ ICP-MS (Thermo)

[0100] a. After ICP-MS is ignited and started, it is stable for about half an hour; pump 1 ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1 μg / L, and the matrix is ​​2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, the oxide (CeO) and the double charge (Ba 2+ ) level, tune the entire mass range (4 ~ 290amu) and achieve the best state; tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charged Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140Ce<2...

Embodiment 3

[0115] Example 3 Production of a kit for detecting the mixed exposure of arsenic and cadmium in pregnant women's whole blood to assist in the diagnosis of low birth weight

[0116]Firstly, the ICP-MS method was used to determine the higher levels of arsenic and cadmium concentrations in the whole blood of the case group, and then the Chi-square test found that there were significant differences in the composition ratios of individuals with different exposure levels between the healthy control group and the case group. The number of people with high exposure was more than that of the control group, and high exposure to arsenic and cadmium significantly increased the incidence of low birth weight. Detection of mixed exposure to arsenic and cadmium can be used for auxiliary diagnosis of low birth weight. This kit includes a batch of reagents and consumables for the detection of arsenic and cadmium in whole blood. The diluent is used for sample dilution, which contains a stable an...

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Abstract

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses detection of arsenic and cadmium mixed exposure as a low birth weight auxiliary diagnosis marker basedon ICP-MS (inductively coupled plasma-mass spectrometry) and application of the marker. The low birth weight related exposure biomarker is the arsenic and cadmium mixed exposure. Arsenic and cadmium concentrations in whole blood are detected by adopting the ICP-MS, and the marker can be used for auxiliary diagnosis of low birth weight; the use amount of the blood is small; the operation is simple,quick and accurate; and the clinical popularization value is relatively good.

Description

field of invention [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to low birth weight and its detection method and application. The exposure biomarker is mixed exposure of arsenic and cadmium, and the detection method is based on ICP-MS Detection method. Background technique [0002] Low birth weight (LBW) is an important cause of neonatal mortality and child development, and has become one of the major risk factors for the global burden of disease. WHO defines low birth weight as birth weight less than 2500 g. Every year, more than 20 million newborns weigh less than 2500 grams, and more than 96% of them are born in developing countries. The incidence of low birth weight in India is as high as 25%, and that in the United States is about 7.6%. The incidence of low birth weight in my country is about 5.9– 11.8%. Possible causes include multiple births, abnormal development of the placenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 夏彦恺孙娴卢婷孙汉卿管权权陈敏健王心如
Owner NANJING MEDICAL UNIV
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