Marker and kit for cardia cancer diagnosis

A technology of markers and kits, applied in the field of medicine and biology, can solve the problems of lack of specificity in detection, and achieve good diagnostic and distinguishing effects

Pending Publication Date: 2021-09-24
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The markers suitable for the prediction of gastric cardia cancer mainly include serum carcinoembryonic antigen (CEA), etc., but after being widely used and further analyzed, it was found that the markers in gastric cancer (49%-60%), lung cancer (52%-77%), breast cancer ( 30%-50%), pancreas (64%), thyroid (60%), and bladder tumors also have CEA, and the detection lacks specificity

Method used

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  • Marker and kit for cardia cancer diagnosis
  • Marker and kit for cardia cancer diagnosis
  • Marker and kit for cardia cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening of Serum Differential Metabolic Markers of Cardiac Cancer

[0035] 1. Experimental samples

[0036] According to strict screening and exclusion criteria, 100 age- and gender-matched healthy subjects (normal control group) and 100 cardiac cancer patients (cardia cancer group) were collected from the First Affiliated Hospital of Zhengzhou University.

[0037] The inclusion criteria for healthy subjects are: no cardiovascular, respiratory, liver, kidney, gastrointestinal, endocrine, blood, mental, or nervous system diseases and history of the above diseases, no acute or chronic diseases, no evidence of any tumor-related , no history of drug allergy, and the results of clinical laboratory tests were within the normal reference range at the time of screening.

[0038] The inclusion criteria for patients with cardiac cancer were as follows: patients with cardiac cancer confirmed by endoscopy and histopathology had not received radiotherapy or chemotherap...

Embodiment 2

[0065] Example 2: Evaluation of the ability of differential metabolite diagnosis to distinguish cardiac cancer patients from healthy individuals

[0066] (1) The ability of a single differential metabolite diagnosis to distinguish cardiac cancer patients from normal individuals:

[0067] 3,4-Dihydroxybenzoneacetic Acid, 1,5- The content data of Anhydro-D-Glucitol and 3-Methylxanthine were analyzed, and the receiver operating curve (ROC curve) was used to evaluate the ability of each differential metabolite to diagnose and distinguish cardiac cancer patients from normal people. 3,4-Dihydroxybenzoneacetic Acid, 1,5-Anhydro-D-Glucitol, and 3-Methylxanthine alone can diagnose the ROC curves for differentiating cardiac cancer patients from normal subjects. figure 2 , image 3 , Figure 4 shown. According to the ROC curve, the area under the curve (AUC), sensitivity and specificity of the ROC curve of each differential metabolite was counted, and the results are shown in Table ...

Embodiment 3

[0087] Example 3: Application of Three Differential Metabolites in Cardiac Cancer Screening

[0088] 1. Collection of serum samples

[0089] According to strict screening and exclusion criteria, 500 healthy subjects (normal control group) and 500 cardiac cancer patients (cardia cancer group) were collected from the First Affiliated Hospital of Zhengzhou University after age and sex matching.

[0090] The inclusion criteria for healthy subjects are: no cardiovascular, respiratory, liver, kidney, gastrointestinal, endocrine, blood, mental, or nervous system diseases and history of the above diseases, no acute or chronic diseases, no evidence of any tumor-related , no history of drug allergy, and the results of clinical laboratory tests were within the normal reference range at the time of screening.

[0091] The inclusion criteria for patients with cardiac cancer were as follows: patients with cardiac cancer confirmed by endoscopy and histopathology had not received radiotherap...

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Abstract

The invention belongs to the technical field of medical biology and particularly discloses a marker and a kit for cardia cancer diagnosis. The marker for cardia cancer diagnosis, provided by the invention, is at least one of 3, 4-Dihydroxybenzeneacetic Acid, 1, 5-Androro-D-Glucitol and 3-Methylxanthine, and a detection reagent of the marker can be used for preparing a cardia cancer diagnosis product. The invention also provides a kit for cardia cancer diagnosis, the kit contains a detection reagent for detecting the marker in the first aspect, and the detection reagent is a reagent for detecting the marker in a sample through chromatography-mass spectrometry or chromatography-mass spectrometry. The cardia cancer can be effectively detected by detecting expression levels of 3, 4-Dihydroxybenenoic Acid, 1, 5-Androdro-D-Glucitol and 3-Methylxanthine in human serum, detection sensitivity is as high as 90.0%, specificity reaches 91.0%, and the kit can be used for large-scale screening of asymptomatic crowds in a cardia cancer high-incidence area and is beneficial to screening and early discovery of asymptomatic high-risk crowds.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses a marker and a kit for diagnosing cardiac cancer. Background technique [0002] Cardiac carcinoma is one of the most common malignant tumors of the upper gastrointestinal tract in northern my country. It is defined as adenocarcinoma within about 2 cm below the esophagogastric junction, also known as esophagogastric junction adenocarcinoma or cardia adenocarcinoma. Historically, my country included cardia cancer as cardia cancer. After the 1980s, cardia cancer was classified as gastric cancer according to pathological classification. However, cardia cancer has relatively independent etiology, clinical manifestations and pathology, and should be treated as an independent disease. tumors for clinical and research purposes. [0003] The pathogenesis of cancer is a process involving many factors and multiple lesions, and cardia cancer is no exception. Studies h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/62
CPCG01N30/02G01N27/62
Inventor 王立东谢叶真李志强徐瑞华魏梦霞韩文莉雷玲玲赵学科宋昕王盼盼杨苗苗胡景峰钟侃王苒
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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