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A kind of preparation method of biological source dipeptidyl peptidase-IV inhibitor

A dipeptidyl peptidase and inhibitor technology, applied in the field of protein extraction, can solve problems such as low resource utilization rate

Active Publication Date: 2021-12-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, the present invention provides a method for preparing a biological source dipeptidyl peptidase-IV inhibitor. The active peptide prepared by the present invention Significant physiological activity, can be used in medicine, functional food, food and other fields, so it has a good application prospect

Method used

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  • A kind of preparation method of biological source dipeptidyl peptidase-IV inhibitor
  • A kind of preparation method of biological source dipeptidyl peptidase-IV inhibitor
  • A kind of preparation method of biological source dipeptidyl peptidase-IV inhibitor

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Pretreatment of Japanese yellow croaker skin:

[0032] (a) washing the fish skin, freeze-drying, and pulverizing to a particle size of 25-50 μm to obtain frozen fish skin powder;

[0033] (b) adding the frozen fish skin powder obtained in step (a) into 0.25mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole sodium hydroxide, First, at 20°C, the ultrasonic power density is 0.25w / cm 2 , ultrasonic treatment for 15min; then at 12°C, ultrasonic power density 1.0w / cm 2 , ultrasonic treatment for 6min; finally at 5°C, ultrasonic power density 2.2w / cm 2 , sonicated for 2 minutes; centrifuged and dried to obtain fish skin powder after removing impurities and defatting;

[0034] (2) Put the fish skin powder in 2wt% acetic acid solution, add pepsin according to the addition amount of 0.01 mg pepsin in every g fish skin powder, extract collagen under the condition of temperature 3 ℃, centrifuge and dry, and obtain...

Embodiment 2

[0050] (1) Pretreatment of Japanese yellow croaker skin:

[0051] (a) After the fish skin is cleaned, freeze-dried, pulverized to a particle size of 50 μm to obtain fish skin frozen powder;

[0052] (b) adding the frozen fish skin powder obtained in step (a) into 0.100mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole potassium hydroxide, First, at 20°C, the ultrasonic power density is 0.35w / cm 2 , ultrasonic treatment for 10min; then at 15°C, ultrasonic power density 0.7w / cm 2 , ultrasonic treatment for 5min; finally at 4°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish skin powder after removing impurities and defatting;

[0053] (2) Put the fish skin powder in 5wt% acetic acid solution, add pepsin according to the addition amount of 0.03mg pepsin in every g fish skin powder, extract collagen under the condition of temperature 5 ℃, centrifuge and dry, and obtain ...

Embodiment 3

[0057] (1) Pretreatment of Japanese yellow croaker skin:

[0058] (a) After the fish skin is cleaned, freeze-dried, pulverized to a particle size of 25 μm to obtain fish skin frozen powder;

[0059] (b) adding the frozen fish skin powder obtained in step (a) into 0.50mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole sodium hydroxide, First, at 25°C, the ultrasonic power density is 0.2w / cm 2 , sonicate for 10min; then at 10°C, ultrasonic power density 1.5w / cm 2 , sonicate for 8min; finally at 8°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish skin powder after removing impurities and defatting;

[0060] (2) Put the fish skin powder in 4wt% acetic acid solution, add pepsin according to the addition amount of 0.02mg pepsin in every g fish skin powder, extract collagen under the condition of temperature 4°C, centrifuge and dry to obtain Japanese Yellow croaker skin c...

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Abstract

The invention relates to the technical field of protein extraction. In order to solve the problem of a large number of by-products and low resource utilization in the processing of Japanese croaker, a method for preparing a biological source dipeptidyl peptidase-IV inhibitor is provided, comprising the following steps: Steps: (1) pretreating the skin of Japanese yellow croaker to obtain skin powder, then extracting collagen from the fish skin powder, and centrifuging and drying to obtain skin collagen of Japanese yellow croaker; (2) using compound enzymes to process ( 1) enzymatic hydrolysis of the obtained Japanese croaker skin collagen to obtain the Japanese croaker skin collagen peptide; (3) screening the Japanese croaker skin collagen peptide with a dipeptidyl peptidase screening kit, namely Obtain biological source dipeptidyl peptidase-IV inhibitor. In the present invention, the dipeptidyl peptidase-IV inhibitory peptide is prepared by using the skin of Japanese yellow croaker fish as the main raw material through a relatively efficient process. Good anti-hyperglycemic effect.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a preparation method of a biological source dipeptidyl peptidase-IV inhibitor. Background technique [0002] Diabetes has become the third metabolic disease that seriously threatens human health and life after malignant tumors and cardiovascular disease. It can cause various complications such as cerebrovascular disease, coronary heart disease, retinopathy, and even death. At present, the control and treatment of diabetes mainly rely on food control and drugs. Drugs for treating diabetes are mainly synthetic drugs, and they all have certain toxic and side effects. [0003] Dipeptidyl peptidase-4 (Dipeptidylpeptidase-IV, DPP-IV) in incretin drugs is a serine protease that can rapidly cleave and inactivate incretins, neuropeptides and cytokines. DPP-4 can specifically cut off the alanine or proline residue at the 2nd position of the N-terminal of the GLP-1 (glucagon-li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78A61P3/10
CPCA61P3/10C12P21/06C07K14/78
Inventor 唐云平江晓霞丁国芳余方苗杨最素黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
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