Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for preparing a biologically derived dipeptidyl peptidase-IV inhibitor

A dipeptidyl peptidase and inhibitor technology, applied in the field of protein extraction, can solve the problem of low resource utilization, and achieve the effects of low addition amount, rapid enzymatic hydrolysis and significant physiological activity

Active Publication Date: 2019-01-11
ZHEJIANG OCEAN UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, the present invention provides a method for preparing a biological source dipeptidyl peptidase-IV inhibitor. The active peptide prepared by the present invention Significant physiological activity, can be used in medicine, functional food, food and other fields, so it has a good application prospect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing a biologically derived dipeptidyl peptidase-IV inhibitor
  • A method for preparing a biologically derived dipeptidyl peptidase-IV inhibitor
  • A method for preparing a biologically derived dipeptidyl peptidase-IV inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Pretreating the skin of Japanese yellow croaker:

[0032] (a) After the fish skin is washed, freeze-dried, and crushed to a particle size of 25-50 μm to obtain frozen fish skin powder;

[0033] (b) Add the frozen fish skin powder obtained in step (a) to 0.25 mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino) ethyl polyethylene glycol bisimidazole sodium hydroxide, First at 20℃, the ultrasonic power density is 0.25w / cm 2 , Ultrasonic treatment for 15min; then at a temperature of 12℃, ultrasonic power density 1.0w / cm 2 , Ultrasonic treatment for 6min; finally at 5℃, the ultrasonic power density is 2.2w / cm 2 , Ultrasonic treatment for 2min; centrifugation, drying, get the fish skin meal after removing impurities and degreasing;

[0034] (2) Put fish skin powder into 2wt% acetic acid solution, add pepsin according to the addition amount of 0.01mg pepsin per gram of fish skin powder, extract collagen at 3℃, centrifuge and dry, get Japan Huanggu fish skin...

Embodiment 2

[0050] (1) Pretreating the skin of Japanese yellow croaker:

[0051] (a) After the fish skin is washed, freeze-dried, and crushed to a particle size of 50 μm to obtain frozen fish skin powder;

[0052] (b) Add the frozen fish skin powder obtained in step (a) to 0.100mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bisimidazole potassium hydroxide, First at 20℃, the ultrasonic power density is 0.35w / cm 2 , Ultrasonic treatment for 10min; then at 15℃, the ultrasonic power density is 0.7w / cm 2 , Ultrasonic treatment for 5min; finally at 4℃ temperature, ultrasonic power density 2.5w / cm 2 , Ultrasonic treatment for 1min; centrifugation, drying, get the fish skin meal after removing impurities and degreasing;

[0053] (2) Put fish skin powder into 5wt% acetic acid solution, add pepsin according to the addition amount of 0.03mg pepsin per g fish skin powder, extract collagen at a temperature of 5℃, centrifuge and dry, get Japan Huanggu fish...

Embodiment 3

[0057] (1) Pretreating the skin of Japanese yellow croaker:

[0058] (a) After the fish skin is washed, freeze-dried, and crushed to a particle size of 25 μm to obtain frozen fish skin powder;

[0059] (b) Add the frozen fish skin powder obtained in step (a) to 0.50mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bisimidazole sodium hydroxide, First, at 25℃, the ultrasonic power density is 0.2w / cm 2 , Ultrasonic treatment for 10min; then at 10℃ temperature, ultrasonic power density 1.5w / cm 2 , Ultrasonic treatment for 8min; finally at 8℃ temperature, ultrasonic power density 2.5w / cm 2 , Ultrasonic treatment for 1min; centrifugation, drying, get the fish skin meal after removing impurities and degreasing;

[0060] (2) Put fish skin powder into 4wt% acetic acid solution, add pepsin according to the addition amount of 0.02mg pepsin per g fish skin powder, extract collagen at 4℃, centrifuge and dry, get Japan Huanggu fish skin collagen; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of protein extraction, which provides a method for preparing a biologically derived dipeptidyl peptidase-IV inhibitor for solving the problems of producinga large number of by-products and low resource utilization ratio in the processing process of Japanese Nibea japonica. The method comprises the following steps: (1) pretreating Nibea japonica skin toobtain fish skin powder, extracting collagen from fish skin powder, centrifugally drying to obtain Nibea japonica skin collagen; (2) enzymatic hydrolysis of the Japanese Nibea skin collagen protein obtained in the step (1) by using complex enzymes to obtain the Japanese Nibea skin collagen peptide; 3. That collagen peptide from the skin of Nibea japonica is screen by a dipeptidyl peptidase screening kit to obtain the biogenic dipeptidyl peptidase-IV inhibitor. Dipeptidyl peptidase is prepare from fish skin of Nibea japonica as main raw material by a relatively high-efficiency process. That dipeptidyl peptidase is prepared from the fish skin of Nibea japonica by the following steps: IV inhibitory peptide, which has a good inhibitory effect on dipeptidyl peptidase-4, has a good anti-hyperglycemia effect.

Description

Technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for preparing a biological dipeptidyl peptidase-IV inhibitor. Background technique [0002] Diabetes has become the third most serious metabolic disease that threatens human health and life after malignant tumors and cardiovascular disease. It can cause cerebrovascular disease, coronary heart disease, retinopathy and other complications, and even death. At present, the control and treatment of diabetes mainly rely on food control and drugs. The drugs used to treat diabetes are mainly synthetic drugs, and they all have certain side effects. [0003] Dipeptidylpeptidase-4 (Dipeptidylpeptidase-IV, DPP-IV) in incretin drugs is a serine protease that can rapidly cleave and inactivate incretin, neuropeptides and cytokines. DPP-4 can specifically cleave the alanine or proline residues at the N-terminal 2 of the GLP-1 (glucagon-like peptide-1) peptide chain, thereby inacti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78A61P3/10
CPCA61P3/10C12P21/06C07K14/78
Inventor 唐云平江晓霞丁国芳余方苗杨最素黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com