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LRSAM1 gene SNP mutation site genotyping primer and application thereof in predication of coronary heart disease

A technology of mutation sites and typing primers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of unverified correlation among Chinese populations

Active Publication Date: 2019-01-11
南京金域医学检验所有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these loci are susceptibility loci of European and American populations, and their correlation with Chinese populations has not yet been verified

Method used

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  • LRSAM1 gene SNP mutation site genotyping primer and application thereof in predication of coronary heart disease
  • LRSAM1 gene SNP mutation site genotyping primer and application thereof in predication of coronary heart disease
  • LRSAM1 gene SNP mutation site genotyping primer and application thereof in predication of coronary heart disease

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Experimental program
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Embodiment 1

[0039] Example 1, a susceptibility gene for coronary heart disease is the LRSAM1 gene. A test sample containing the gene of LRSAM1 can be obtained from the blood of the subject.

Embodiment 2

[0040] Embodiment 2, a reagent for in vitro detection of coronary heart disease-related genes, the reagent is used to detect the SNP genotype of the LRSAM1 gene rs3802358 site, the reagent includes the following primers and probes:

[0041] Upstream primer sequence 5'ATCCTGAAATGTAAGCAAATGACTGT3' (SEQ ID No.1);

[0042] Downstream primer sequence 5'GCCAGGATCCAGCCAGGTA3' (SEQ ID No.2);

[0043] G-type probe sequence 5'VIC-CCACACATACGGCTG-MGB3' (SEQ ID No.3);

[0044] Type A probe sequence 5'FAM-CCACACATATGGCTG-MGB3' (SEQ ID No.4).

[0045] The so-called SNP is single nucleotide polymorphism, which mainly refers to the DNA sequence polymorphism caused by the variation of a single nucleotide at the genome level. It is the most common type of heritable variation in humans, accounting for more than 90% of all known polymorphisms. Some SNPs will directly affect the protein structure or gene expression level, and may themselves be candidate alteration sites for the genetic mechanis...

Embodiment 3

[0048] Embodiment 3, a preparation or kit with reagents for in vitro detection of coronary heart disease-related genes is characterized in that the kit includes PCR amplification enzyme (ie, DNA polymerase) and corresponding buffer. The kit for detecting coronary heart disease-related genes with rs3802358 polymorphism can be used for in vitro detection of coronary heart disease-related gene polymorphisms; the kit for coronary heart disease-related genes with mutations at rs3802358 can be used for detection, prevention, Diagnosis or treatment of coronary heart disease; the method for detecting coronary heart disease-related genes in vitro can be used for detection, prevention, diagnosis or treatment of coronary heart disease.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to an LRSAM1 gene SNP mutation site genotyping primer and application thereof in predication of coronary heart disease, comprising the following steps: (1) a primer for amplifying the rs3802358 site: an upstream primer sequence 5'ATCCTGAAATGTAAGCAAATGACTGT3'; a downstream primer sequence 5'GCCAGGATCCAGCCAGGTA3'; (2)a probe for performing mutation sit genotyping: a G type probe sequence 5'CCACACATACGGCTG3'; an A type probe sequence 5'CCACACATATGGCTG3'. The invention discloses a new LRSAM1 gene which can explain the hereditary basis of coronary heart disease of Chinese Han population and is related to susceptibility of coronary heart disease, and meanwhile confirms an SNP site rs3802358 closely related to susceptibility of coronary heart disease on the LRSAM1 gene,and is high in test precision and sensitivity.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site on the LRSAM1 gene associated with susceptibility to coronary heart disease, and the corresponding in vitro detection for detecting the SNP site The reagent, especially the application of the nucleic acid affinity ligand of the SNP site in the preparation of a composition for detecting, screening or predicting the susceptibility of coronary heart disease in Han population. Background technique [0002] Coronary heart disease (CHD), referred to as coronary heart disease, is a disease with complex etiology. Both environmental and genetic factors are related to the occurrence and development of coronary heart disease. In the United States and many developed countries, coronary heart disease ranks first among the causes of death. At present, the mortality rate of coronary heart disease in my country also remains...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 毛源袁杭曹鹏郑伟
Owner 南京金域医学检验所有限公司
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