Systems and methods for 4-d hyperspectrial imaging

Abstract

Systems and methods for hyperspectral imaging are described. A hyperspectral imaging system (300), comprising: a sample holder (370) configured to hold a sample; a light source (3010) configured to emit excitation light (402) having one or more wavelengths; a two-dimensional imaging device (380); and an optical system comprising: a first spatial light modulator (320a); a first dispersive element (340a); and a second dispersive element (340b); wherein the optical system is configured to (i) structure the excitation light (402), using the first spatial light modulator (320a), into a predetermined two-dimensional excitation pattern at a conjugate plane of a focal plane in the sample; (ii) spectrally disperse, using the first dispersive element (340a), the excitation light (404); (iii) use excitation pattern (406) to illuminate the sample illuminate the sample in an excitation pattern (406) with the one or more wavelengths dispersed along a first lateral direction within the focal plane; (iv) collect, from the sample, emission light (408); (v) spectrally disperse, using the second dispersive element (340b), the collected emission light (408); and (vi) provide the collected emission light (410) in-focus to the imaging device (380) such that the spectrally dispersed emission light (410) is spectrally dispersed along a second lateral direction.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 342,252, filed May 27, 2016, and U.S. Non-Provisional Application No. 15 / 607,457, filed May 27, 2017, the contents of which are incorporated by reference. technical field [0003] The present disclosure relates generally to the field of imaging and microscopy systems and methods. More specifically and without limitation, the disclosed embodiments relate to systems and methods for hyperspectral imaging through the use of spatial light modulators and dispersive elements. Background technique [0004] Hyperspectral imaging is a remote sensing spectroscopy method commonly used in non-medical applications such as materials identification, astronomy, surveillance, and geophysical applications. This is an "imaging spectroscopy" approach that combines the spatial resolution of imaging with the chemical specificity of spectroscopy. Light collected from...

AI Technical Summary

Problems solved by technology

Also, if one narrowband filter is used at a time, most of the collected fluorescence will be discarded

Therefore, the process is inefficient and can even lead to photobleaching of the fluorophores in the sample, making them never able to fluoresce

Images