Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof

A digestive tract, short peptide technology, applied in the field of biotechnology and medicine, can solve problems such as difficult to cure, achieve excellent efficacy, no toxic side effects

Active Publication Date: 2019-01-15
FUJIAN LANHAO BIOTECH
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing medical technology, it is difficult to cure, mainly relying on prevention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof
  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof
  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the amino acid sequence of the side chain protection short peptide, Ile-Pro-Asp-Asp-Val -Ala or Pro-Asp-Asp-Val-Ala or Ile-Pro-Asp-Asp-Val or Leu-Pro-Asp-Asp-Val-Ala or Val-Pro-Asp-Asp-Val-Ala or Ile-Ser -Asp-Asp-Val-Ala or Val-Ser-Asp-Asp-Val-Ala or Leu-Ser-Asp-Asp-Val-Ala or Ile-Pro-Glu-Asp-Val-Gly or Ile-Pro-Glu -Asn-Leu-Ala or Ile-Pro-Gln-Asp-Ile-Gly as the main sequence, truncated from any position into a short peptide consisting of 3 or 4 or 5 amino acids with the same biological activity as the short peptide Fragments, short peptide analogs, short peptide derivat...

specific Embodiment approach

[0050] The short peptides of the present invention and their analogs, derivatives and fragments (P1~P11, P1 analogs, P1 derivatives (modified with phosphate groups), and fragments of P1) are chemically synthesized by the solid-phase Fmoc method, and purified by HPLC , with a purity of more than 97%, the following animal experiments were carried out.

[0051] Among them, P1 is Ile-Pro-Asp-Asp-Val-Ala, P2 is Pro-Asp-Asp-Val-Ala, P3 is Ile-Pro-Asp-Asp-Val, P4 is Leu-Pro-Asp-Asp- Val-Ala, P5 is Val-Pro-Asp-Asp-Val-Ala, P6 is Ile-Ser-Asp-Asp-Val-Ala, P7 is Val-Ser-Asp-Asp-Val-Ala, P8 is Leu- Ser-Asp-Asp-Val-Ala, P9 is Ile-Pro-Glu-Asp-Val-Gly, P10 is Ile-Pro-Glu-Asn-Leu-Ala, P11 is Ile-Pro-Gln-Asp-Ile- Gly; P1 analogue is Ile-Pro-Asp-Asp-Val-Lys, P1 derivative is Ile-Pro-Asp-Asp-Val p -Ala, the fragment of P1 is Ile-Pro-Asp-Asp.

Embodiment 1

[0052] Example 1 The experiment of seaweed short peptide inhibiting the effect of intestinal inflammation on the weight change of mice

[0053] The mice in this experiment were divided into 15 groups, with 6 mice in each group. They were given drinking water for 7 days to adapt to the environment, and then 2.5% DSS solution for 5 days to establish an acute intestinal inflammation model, and then replaced with tap water for small Rats drank ad libitum. From the first day of the experiment, each mouse in each group was given the short peptide series involved in the present invention, and at the same time every day, each group of mice was gavaged with a short peptide of 1 mg / mL, and the amount of gavage was 200 μL. The comparison of the results of the negative control group verifies the biological activity of the bioactive peptides in the treatment of intestinal inflammation. The mice that have established the model will suffer from loss of appetite or cannot eat normally due to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a seaweed-derived short peptide capable of inhibiting the inflammations of a digestive tract and application thereof. The amino acid main sequences of the short peptide fragments, analogs, derivatives and variants of the short peptide bear 70% or more homogeneity and 90% or more similarity to the main amino acid sequence of the short peptide. The short peptide, a nucleotide,the short peptide fragments, the short peptide analogs, the short peptide derivatives and the short peptide variants are used for preparing drugs or health care products capable of inhibiting the inflammations of the digestive tract and for preparing drugs or health care products capable of treating the inflammations of the digestive tract, i.e., gastritis, treating intestinal inflammation including colonic inflammations and rectal inflammations, and treating mucosal inflammations such as esophagitis. The short peptide of the invention has a clear molecular structure; production technology ismature; the prepared finished product is non-toxic, harmless and free of side effects, has the remarkable feature of capacity of inhibiting the inflammations of the digestive tract and repairing themucosa of the digestive tract, and is an excellent drug for inhibiting the inflammations of the digestive tract.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide derived from seaweed for inhibiting digestive tract inflammation and its application. Background technique [0002] Gastritis refers to the inflammatory disease of gastric mucosa, which is divided into two types: acute and chronic gastritis; it is also divided into Helicobacter pylori-associated gastritis, stress gastritis, autoimmune gastritis, etc. Acute gastritis can be divided into simple, erosive and hemorrhagic, corrosive, suppurative gastritis according to its pathological changes, and chronic gastritis can be divided into three categories: non-atrophic, atrophic and special type gastritis according to its pathological changes. Regardless of the classification, the pathogenesis and healing process of gastritis is through epithelial injury, mucosal inflammation and epithelial regeneration. The existing drugs for treating gastritis m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/06A61K38/08A61P29/00A61P1/00A61P1/04A23L33/18
CPCA61P1/00A61P1/04A61P29/00A23L33/18C07K7/06A23V2002/00A61K38/00A23V2200/32A23V2250/55
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com