Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof
A digestive tract, short peptide technology, applied in the field of biotechnology and medicine, can solve problems such as difficult to cure, achieve excellent efficacy, no toxic side effects
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[0033] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to an inert solid phase carrier one by one, and then use a strong acid to cleave the peptide chain from the carrier while removing the amino acid sequence of the side chain protection short peptide, Ile-Pro-Asp-Asp-Val -Ala or Pro-Asp-Asp-Val-Ala or Ile-Pro-Asp-Asp-Val or Leu-Pro-Asp-Asp-Val-Ala or Val-Pro-Asp-Asp-Val-Ala or Ile-Ser -Asp-Asp-Val-Ala or Val-Ser-Asp-Asp-Val-Ala or Leu-Ser-Asp-Asp-Val-Ala or Ile-Pro-Glu-Asp-Val-Gly or Ile-Pro-Glu -Asn-Leu-Ala or Ile-Pro-Gln-Asp-Ile-Gly as the main sequence, cut from any position into a short peptide consisting of 3 or 4 or 5 amino acids with the same biological activity as the short peptide Fragments, short peptide analogs, short peptide derivative...
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[0050] The short peptide of the present invention and its analogues, derivatives and fragments (P1~P11, P1 analogues, P1 derivatives (modified with phosphoric acid group), P1 fragments) were chemically synthesized by solid phase Fmoc method, and purified by HPLC , The purity is over 97%, and the following animal tests are carried out.
[0051] Among them, P1 is Ile-Pro-Asp-Asp-Val-Ala, P2 is Pro-Asp-Asp-Val-Ala, P3 is Ile-Pro-Asp-Asp-Val, and P4 is Leu-Pro-Asp-Asp- Val-Ala, P5 is Val-Pro-Asp-Asp-Val-Ala, P6 is Ile-Ser-Asp-Asp-Val-Ala, P7 is Val-Ser-Asp-Asp-Val-Ala, P8 is Leu- Ser-Asp-Asp-Val-Ala, P9 is Ile-Pro-Glu-Asp-Val-Gly, P10 is Ile-Pro-Glu-Asn-Leu-Ala, P11 is Ile-Pro-Gln-Asp-Ile- Gly; P1 analogue is Ile-Pro-Asp-Asp-Val-Lys, P1 derivative is Ile-Pro-Asp-Asp-Val p -The fragment of Ala, P1 is Ile-Pro-Asp-Asp.
Example Embodiment
[0052] Example 1 Experiment of the effect of seaweed short peptide on inhibiting intestinal inflammation on weight change of mice
[0053] The mice in this experiment were divided into 15 groups, each with 6 mice. The mice were used for 7 days of drinking water to adapt to the environment, and then 2.5% DSS solution was used for 5 days to establish an acute intestinal inflammation model, and then they were replaced with tap water. Rats drink freely. From the first day of the experiment, each mouse in each group was given the short peptide series of the present invention, and each group of mice was gavaged with 1 mg / mL short peptide at the same time every day. The gavage amount was 200 μL. The comparison of the results of the negative control group verifies the biological activity of the bioactive peptides in the treatment of intestinal inflammation. The mice that have completed the model will suffer from loss of appetite or unable to eat normally due to the inflammation of the en...
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