Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof

A digestive tract, short peptide technology, applied in the field of biotechnology and medicine, can solve problems such as difficult to cure, achieve excellent efficacy, no toxic side effects

Active Publication Date: 2019-01-15
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing medical technology, it

Method used

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  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof
  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof
  • Seaweed-derived short peptide capable of inhibiting inflammations of digestive tract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to an inert solid phase carrier one by one, and then use a strong acid to cleave the peptide chain from the carrier while removing the amino acid sequence of the side chain protection short peptide, Ile-Pro-Asp-Asp-Val -Ala or Pro-Asp-Asp-Val-Ala or Ile-Pro-Asp-Asp-Val or Leu-Pro-Asp-Asp-Val-Ala or Val-Pro-Asp-Asp-Val-Ala or Ile-Ser -Asp-Asp-Val-Ala or Val-Ser-Asp-Asp-Val-Ala or Leu-Ser-Asp-Asp-Val-Ala or Ile-Pro-Glu-Asp-Val-Gly or Ile-Pro-Glu -Asn-Leu-Ala or Ile-Pro-Gln-Asp-Ile-Gly as the main sequence, cut from any position into a short peptide consisting of 3 or 4 or 5 amino acids with the same biological activity as the short peptide Fragments, short peptide analogs, short peptide derivative...

Example Embodiment

detailed description

[0050] The short peptide of the present invention and its analogues, derivatives and fragments (P1~P11, P1 analogues, P1 derivatives (modified with phosphoric acid group), P1 fragments) were chemically synthesized by solid phase Fmoc method, and purified by HPLC , The purity is over 97%, and the following animal tests are carried out.

[0051] Among them, P1 is Ile-Pro-Asp-Asp-Val-Ala, P2 is Pro-Asp-Asp-Val-Ala, P3 is Ile-Pro-Asp-Asp-Val, and P4 is Leu-Pro-Asp-Asp- Val-Ala, P5 is Val-Pro-Asp-Asp-Val-Ala, P6 is Ile-Ser-Asp-Asp-Val-Ala, P7 is Val-Ser-Asp-Asp-Val-Ala, P8 is Leu- Ser-Asp-Asp-Val-Ala, P9 is Ile-Pro-Glu-Asp-Val-Gly, P10 is Ile-Pro-Glu-Asn-Leu-Ala, P11 is Ile-Pro-Gln-Asp-Ile- Gly; P1 analogue is Ile-Pro-Asp-Asp-Val-Lys, P1 derivative is Ile-Pro-Asp-Asp-Val p -The fragment of Ala, P1 is Ile-Pro-Asp-Asp.

Example Embodiment

[0052] Example 1 Experiment of the effect of seaweed short peptide on inhibiting intestinal inflammation on weight change of mice

[0053] The mice in this experiment were divided into 15 groups, each with 6 mice. The mice were used for 7 days of drinking water to adapt to the environment, and then 2.5% DSS solution was used for 5 days to establish an acute intestinal inflammation model, and then they were replaced with tap water. Rats drink freely. From the first day of the experiment, each mouse in each group was given the short peptide series of the present invention, and each group of mice was gavaged with 1 mg / mL short peptide at the same time every day. The gavage amount was 200 μL. The comparison of the results of the negative control group verifies the biological activity of the bioactive peptides in the treatment of intestinal inflammation. The mice that have completed the model will suffer from loss of appetite or unable to eat normally due to the inflammation of the en...

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Abstract

The invention provides a seaweed-derived short peptide capable of inhibiting the inflammations of a digestive tract and application thereof. The amino acid main sequences of the short peptide fragments, analogs, derivatives and variants of the short peptide bear 70% or more homogeneity and 90% or more similarity to the main amino acid sequence of the short peptide. The short peptide, a nucleotide,the short peptide fragments, the short peptide analogs, the short peptide derivatives and the short peptide variants are used for preparing drugs or health care products capable of inhibiting the inflammations of the digestive tract and for preparing drugs or health care products capable of treating the inflammations of the digestive tract, i.e., gastritis, treating intestinal inflammation including colonic inflammations and rectal inflammations, and treating mucosal inflammations such as esophagitis. The short peptide of the invention has a clear molecular structure; production technology ismature; the prepared finished product is non-toxic, harmless and free of side effects, has the remarkable feature of capacity of inhibiting the inflammations of the digestive tract and repairing themucosa of the digestive tract, and is an excellent drug for inhibiting the inflammations of the digestive tract.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide derived from seaweed for inhibiting digestive tract inflammation and its application. Background technique [0002] Gastritis refers to the inflammatory disease of gastric mucosa, which is divided into two types: acute and chronic gastritis; it is also divided into Helicobacter pylori-associated gastritis, stress gastritis, autoimmune gastritis, etc. Acute gastritis can be divided into simple, erosive and hemorrhagic, corrosive, suppurative gastritis according to its pathological changes, and chronic gastritis can be divided into three categories: non-atrophic, atrophic and special type gastritis according to its pathological changes. Regardless of the classification, the pathogenesis and healing process of gastritis is through epithelial injury, mucosal inflammation and epithelial regeneration. The existing drugs for treating gastritis m...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P29/00A61P1/00A61P1/04A23L33/18
CPCA61P1/00A61P1/04A61P29/00A23L33/18C07K7/06A23V2002/00A61K38/00A23V2200/32A23V2250/55
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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