A kind of in vitro propagation method of red ginseng
A red ginseng and in vitro technology, which is applied to the field of in vitro propagation of red ginseng, can solve the problems of difficulty in large-scale propagation, slow growth of red ginseng seedlings and germination season, and achieves short reproduction cycle, low cost, and high quality and survival rate. Effect
Active Publication Date: 2022-03-08
丽江市古城区秋成种养殖有限公司
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Problems solved by technology
[0004] The object of the present invention is to provide a method for in vitro propagation of red ginseng, which solves the problem that red ginseng seedlings grow slowly and are limited by the germination season, making it difficult to carry out large-scale propagation
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Embodiment 1
[0033] 1. Acquisition of explants: Select healthy plants with good growth potential, no damage by diseases and insect pests, and no deformities, and get the 3-5 stem segments under the shoot tip, and cut them into about 2-3cm long with a scalpel.
[0034] 2. Clean the soil and dust on the surface of the budded stem section in step 1 with tap water, then rinse it under running water for 2 hours, put it on an ultra-clean workbench and disinfect it with 75% (volume ratio) alcohol for 60 seconds, and then use 0.1 %HgCl2 (mass ratio) sterilized for 6 minutes, finally rinsed with sterile water for 8 times, and then placed on sterile filter paper to blot the surface moisture. Shake the utensils well throughout the sanitization process.
[0035] 3. Callus induction: Cut the stem segment with buds that have been sterilized in 2 steps as material, cut into 1.5-2.0cm long, and insert it into the following MS basic medium A:
[0036]
[0037] Under the conditions of light intensity of...
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The invention discloses a method for in vitro propagation of red fruit ginseng. Firstly, callus induction is carried out, and red fruit ginseng explants after disinfection and sterilization are inoculated in MS basic medium A, and callus appears after culturing. The tissue was cut and transferred to MS basic medium B for culture, the callus continued to proliferate, and clustered shoots differentiated on the surface, and the clustered shoots were cut and transferred to MS basic medium C; the clustered shoots were inoculated in turn according to the growth cycle of 40 days Add MS basic medium B and MS basic medium C, as a culture method for the differentiation and proliferation of clustered buds of red ginseng, and repeatedly subculture for 3 to 4 generations to obtain seedlings; take the robust main seedlings with a height of 3-4cm in the seedlings for inoculation Cultivate in MS basic medium D to obtain rooted seedlings with strong seedlings and thick roots; take the rooted seedlings and place them at room temperature to harden the seedlings and transplant them to sterilized humus for heat preservation and moisture cultivation. After growing for 30 days, rooted transplanted seedlings are obtained. The beneficial effect of the invention is that the red fruit ginseng propagation method has low cost, short propagation cycle, high quality and survival rate.
Description
technical field [0001] The invention belongs to the technical field of Chinese herbal medicine propagation, and relates to a method for in vitro propagation of red ginseng. Background technique [0002] The scientific name of red fruit ginseng is Campanumoealancifolia (Roxb.) Merr., which is a perennial plant of Campanulaceae (Campanulaceae). In some areas south of the Yangtze River, it grows on grassy slopes, ditch sides or forests at an altitude of 300-1800m. It is a dual-purpose plant for medicine and food, and it also has strong ornamental value. The root of red ginseng is used as medicine. It tastes sweet, slightly bitter, and flat in nature. It has the effects of nourishing deficiency and Qi, eliminating phlegm and relieving pain. The fruit of red ginseng is also edible. It mainly contains polysaccharides, natural fibers, crude protein, crude fat and minerals, etc. The polysaccharide content is as high as 45.80%. Oxidation has obvious inhibitory effect. [0003] Red...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 黄衡宇李文忠王美蓉
Owner 丽江市古城区秋成种养殖有限公司