Breeding method of rainbow trout triploids through hydrostatic pressure induction

A triploid, hydrostatic pressure technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of large damage to fertilized eggs, low survival rate, unsustainable stability, etc., and achieves short processing time and survival. High efficiency and simple operation

Pending Publication Date: 2019-01-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used fish polyploid induction methods include the direct method, which uses biological methods, physical methods and chemical methods to directly induce triploid fish, of which the temperature shock method and hydrostatic pressure method are the most commonly used physical methods; The method refers to the induction method of tetraploid and diploid hybridization to produce triploid. The indirect method requires obtaining a certain number of tetraploid rainbow trout populations. Related studies have shown that the survival rate of tetraploid hybrid offspring is not high, so it cannot be sustained. Stable large-scale triploid production of rainbow trout
Based on a large number of reports, among the direct methods to induce triploid rainbow trout, the most widely used method in my country is the temperature shock method. The temperature treatment is relatively simple and does not require special equipment. % of the induction rate, and the heat shock treatment is not stable, the damage to the fertilized eggs is greater, so that the survival rate is not high, compared with the heat shock method, the hydrostatic pressure method is relatively less stimulating to the fertilized eggs, and the treatment time is shorter , is also more stable, and the effect is better than that of heat shock
However, at present, my country still has not formed a relatively mature polyploid induction technology system for rainbow trout.

Method used

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  • Breeding method of rainbow trout triploids through hydrostatic pressure induction
  • Breeding method of rainbow trout triploids through hydrostatic pressure induction
  • Breeding method of rainbow trout triploids through hydrostatic pressure induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Rainbow trout triploid breeding technology method

[0052] The seed production technique method comprises the following steps:

[0053] 1 Materials and methods

[0054] Adopt the single factor experiment method, under the condition of water temperature 10.5 ℃, set 9 gradients in the time range of 10 to 30 minutes after fertilization: starting time 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30 Minutes, respectively named three 1... three 9 groups, select 68.9kg / cm 2 The magnitude of the hydrostatic pressure and the induction intensity lasting 5 minutes were used to induce triploid rainbow trout, followed by normal hatching, flotation, and seedling cultivation, and the indicators such as the rate of eyes, flotation rate, and triploid rate of each group were counted.

[0055] 2 Subjects

[0056] The experiment started on November 30, 2017. The experiment site was Linqu Salmon Fishery Development Co., Ltd., Weifang City, Shandong Province. The 3- and 4-year-ol...

Embodiment 2

[0070] Embodiment 2: rainbow trout triploid breeding technology method

[0071] The seed production technique method comprises the following steps:

[0072] 1 Materials and methods

[0073] Adopt the single factor experiment method, set 13 gradients within the time range of 10 to 40 minutes after fertilization under the condition of water temperature 10.5°C: starting time 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30 , 32.5, 35, 37.5, 40 minutes, respectively named as three 1···three 13 groups, select 65kg / cm 2 The magnitude of the hydrostatic pressure and the induction intensity lasting 5 minutes were used to induce triploid rainbow trout, followed by normal hatching, flotation, and seedling cultivation, and the indicators such as the rate of eyes, flotation rate, and triploid rate of each group were counted.

[0074] 2 Subjects

[0075] The experiment started on December 20, 2017. The experiment site was Linqu Salmon Fishery Development Co., Ltd., Weifang City, Shandong Prov...

Embodiment 3

[0089] Embodiment 3: Rainbow trout triploid breeding technology method

[0090] The seed production technique method comprises the following steps:

[0091] 1 Materials and methods

[0092] Adopting the orthogonal experiment method, the experiment was carried out on the three main factors affecting the experimental results: the starting time, the size of the hydrostatic pressure, and the duration. Each factor was set at three levels, which were respectively the starting time (20, 30, 40 minutes ), pressure (60, 65, 70kg / cm 2 ), duration (3, 5, 7 minutes) (Table 1). Under the condition of water temperature of 10.5 ℃, normal hatching, flotation, and seedling cultivation were carried out, and the indicators such as the rate of eyes, flotation rate, and triploid rate of each group were counted.

[0093] Table 1 Orthogonal experiment scheme for induction of triploid rainbow trout

[0094]

[0095] 2 Subjects

[0096] The experiment started on January 10, 2017. The experimen...

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Abstract

The invention discloses a breeding method of rainbow trout triploids through hydrostatic pressure induction. A certain time interval is selected at a proper time after sperm-egg binding so as to carryout hydrostatic pressure treatment with certain intensity on fertilized eggs, and then a normal environment is recovered for conventional incubation, so that the fertilized eggs which can be bred into diploids generate 100% triploid fertilized eggs after hydrostatic pressure treatment, and then the triploid fertilized eggs are bred into triploid fry. The method has the advantages that a built rainbow trout triploid breeding technical method can be used for generating the triploid rainbow trout fry through efficient batch induction, the induction rate of the rainbow trout triploids is up to 100%, and the survival rate of the rainbow trout triploids is not obviously different from that of diploid rainbow trout. The method is easy to operate, high in practicability, easy to implement, safe and reliable, and provides technical support for large-scale production of the rainbow trout triploids.

Description

technical field [0001] The invention belongs to the technical field of fish polyploid breeding, and relates to a method for producing rainbow trout triploid seeds induced by hydrostatic pressure, and a technical method for mass-producing rainbow trout triploid fry by establishing a hydrostatic pressure method to induce doubling of fertilized egg chromosome groups , to determine the best induction conditions, to achieve the purpose of industrial production of sterile triploids, so as to make up for the deficiencies of the prior art. Background technique [0002] Rainbow trout (Onchorynchus mykiss) belongs to Salmoniformes (Salmoniformes), Salmonidae (Salmonidae), and salmon genus (Onchorynchus). rainbow trout, native to North America, is a typical cold-water fish, one of the world's most economically farmed fish and an important research object in the field of aquatic genetics and breeding. my country began to introduce and breed rainbow trout in 1959. Due to long-term inbre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 温海深陈落落车德钊李吉方辛苑茹张美昭
Owner OCEAN UNIV OF CHINA
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