Sialic acid and extraction method thereof

An extraction method and technology of sialic acid, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve the effect of reducing degradation and high yield

Inactive Publication Date: 2019-01-18
武汉中科光谷绿色生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of acid hydrolysis, the sialic acid hydrolyzed under the condition of high temperature and strong acid for a long time is easy to degrade and generate impurities

Method used

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  • Sialic acid and extraction method thereof
  • Sialic acid and extraction method thereof
  • Sialic acid and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The 10g / L polysialic acid fermentation broth is filtered through a 100nm ceramic membrane to obtain the permeate, and the permeate is filtered through a 5KDa ultrafiltration membrane to remove small molecular compounds, so that the conductivity of the solution is less than 2000μS / cm, polysialic acid is in the ultra-strong The solid acid fixed bed was subjected to the hydrolysis reaction for 20 minutes, and the temperature of the hydrolysis reaction was 100°C.

[0031] After hydrolysis, use membrane filtration with a specification of 0.8KDa to obtain the sialic acid monomer solution and membrane filtration retentate, add 0.5% activated carbon to the sialic acid monomer solution and filter to obtain a decolorization solution, and put the membrane filtration retentate into a super strong solid acid for fixation The bed undergoes a secondary hydrolysis reaction. The decolorization solution was heated and concentrated under vacuum, then sulfuric acid was added until the pH o...

Embodiment 2

[0033] The 15g / L polysialic acid fermentation broth is filtered through a 150nm ceramic membrane to obtain a permeate, and the permeate is filtered through an 8KDa ultrafiltration membrane to remove small molecular compounds so that the conductivity of the solution is less than 2000μS / cm The solid acid fixed bed was subjected to the hydrolysis reaction for 25 minutes, and the temperature of the hydrolysis reaction was 90°C.

[0034] After hydrolysis, use membrane filtration with a specification of 0.9KDa to obtain the sialic acid monomer solution and membrane filtration retentate, add 1.5% activated carbon to the sialic acid monomer solution and filter to obtain a decolorization solution, and put the membrane filtration retentate into super strong solid acid for fixation The bed undergoes a secondary hydrolysis reaction. The decolorization solution was heated and concentrated in vacuum, then added glacial acetic acid until the pH of the solution was 2, acidified and crystalliz...

Embodiment 3

[0036]The 20g / L polysialic acid fermentation broth is filtered through a 200nm ceramic membrane to obtain the permeate, and the permeate is filtered through a 10KDa ultrafiltration membrane to remove small molecular compounds so that the conductivity of the solution is less than 2000μS / cm The solid acid fixed bed was subjected to the hydrolysis reaction for 30 minutes, and the temperature of the hydrolysis reaction was 85°C.

[0037] After hydrolysis, use a membrane filter with a specification of 1.0KDa to obtain the sialic acid monomer solution and membrane filtration retentate, add 2.5% activated carbon to the sialic acid monomer solution and filter to obtain a decolorization solution, and put the membrane filtration retentate into a super strong solid acid for fixation The bed undergoes a secondary hydrolysis reaction. The decolorization solution was heated and concentrated under vacuum, and then sulfuric acid was added until the pH of the solution was 1, acidified and crys...

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Abstract

The invention relates to sialic acid and an extraction method thereof and belongs to the technical field of sialic acid extraction. The method comprises the step of subjecting polysialic acid to a hydrolysis reaction for 10 to 40 minutes on a super-strength solid acid fixed bed. According to the sialic acid and the extraction method thereof, reaction time of the polysialic acid on the super-strength solid acid fixed bed is controlled to reduce degradation of the sialic acid, so that the purity of the sialic acid prepared from the polysialic acid through hydrolysis reaches 98% or more, and theyield of the sialic acid reaches 70% or more.

Description

technical field [0001] The invention belongs to the technical field of sialic acid extraction, and in particular relates to a sialic acid and an extraction method thereof. Background technique [0002] The preparation methods of sialic acid mainly include chemical synthesis, enzyme synthesis, natural product extraction and microbial fermentation. The microbial method obtains polysialic acid through fermentation, and then hydrolyzes the polysialic acid to obtain sialic acid monomers. [0003] The method commonly used in the prior art is to take the supernatant after the fermentation broth is centrifuged, add ethanol or complexing agent to remove protein precipitation, remove endotoxin by ion exchange column or the mode of passing membrane, obtain polysialic acid, polysialic acid ( PSA) is passed through acid hydrolysis again, the step of decolouring, finally crystallizes with organic solvents such as ethyl acetate, glacial acetic acid. However, in the process of acid hydrol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H1/00C07H7/033
CPCC07H1/00C07H1/06C07H7/033
Inventor 李翔宇张力文陆姝欢马凡提刘宏荣陈祥松王刚
Owner 武汉中科光谷绿色生物技术有限公司
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