A kind of primer, kit and method for distinguishing mycoplasma hyopneumoniae wild strain and vaccine strain

Abstract

The invention belongs to the technical field of molecular diagnosis of animal pathogens, and in particular relates to a primer, a kit and a method for differentiating Mycoplasma hyopneumoniae wild strains and vaccine strains, including primer pairs for detecting Mycoplasma hyopneumoniae wild strains and vaccine strains, detecting The primer pair sequence of Mycoplasma hyopneumoniae wild strain is: SEQ ID NO.1 and SEQ ID NO.2, the primer pair sequence of detecting Mycoplasma hyopneumoniae vaccine strain is: SEQ ID NO.3 and SEQ ID NO.4, and its primer The targeted target nucleotide is small, with high sensitivity and strong specificity, which can effectively reduce the probability of false positive and false negative and eliminate the interference of other substances.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis of animal pathogens, and in particular relates to a primer, a kit and a method for distinguishing wild strains and vaccine strains of mycoplasma hyopneumoniae. Background technique [0002] Mycoplasma hyopneumoniae (Mhp) is the main pathogen that causes mycoplasma pneumonia in swine, and is also one of the main primary pathogens of porcine respiratory syndrome. The infection rate of Mhp in pig farms is 30%-80%, which can mainly cause the growth of pigs to be hindered, the growth retardation and the feed conversion rate to be greatly reduced, and the production performance to be significantly reduced. It is the most common cause of serious economic losses to the pig industry in my country. It is one of the most important epidemic diseases that occur, spread widely and are most difficult to purify. [0003] At present, the traditional detection methods of Mhp virus include pathogen isola...

AI Technical Summary

Problems solved by technology

[0012] 1. It is difficult and time-consuming to isolate and cultivate pathogens

[0013] 2. Serological detection technology: prone to false positive or false negative results

[0014] 3. Conventional PCR: (1) requires many instruments and equipment; (2) complex operation and long operation time; (3) very easy to pollute; (4) high technical operation requirements, difficult to promote at the grassroots level; (5) quality control Not in place, difficult to standardize

[0015] 4. Common PCR and single-plex fluorescent PCR cannot distinguish Mhp wild strains and vaccine strains

[0017] 1. The serological detection method is antigen and antibody reaction, and only one detection cannot accurately judge the infection status and detoxification status of the group

[0018] 2. Serological detection of antibodies, the level of detected antibodies cannot be quantitatively detected and analyzed, and serology can only evaluate the level of humoral immunity in the body

[0019] 3. There are technical bottlenecks in serological assessment of population health and assessment of asymptomatic virus transmission and immune tolerance

[0020] 4. The operation of ordinary PCR itself is more complicated and the operation time is longer

[0022] 6. Other etiological detection methods (such as qPCR, etc.) are widely used in laboratories but less in clinical applications, and it is difficult to rule out the interference of other substances, resulting in inaccurate results

The identification of the above-mentioned pathogenic wild strains and vaccine strains is realized by conventional PCR, and the electrophoresis is used to distinguish the size of the amplified bands. Differentiation is also likely to cause contamination of amplification products, thereby affecting accuracy

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