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Ion chromatography detection method of gentamicin B purity

The technology of a gentamicin and detection method is applied in the field of ion chromatography detection of the purity of gentamicin B, which can solve problems such as unfavorable product quality of enterprises, no discovery of gentamicin B, and control, and achieve high accuracy , Simple operation and the effect of improving detection efficiency

Inactive Publication Date: 2019-01-22
WUXI FORTUNE PHARMA
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Problems solved by technology

[0003] By consulting a large number of Chinese and foreign literature and patents, there is no literature report on the determination of the purity of gentamicin B by a method similar to ion chromatography, which is not conducive to the company's control of product quality, so an effective method for the determination of gentamicin B is urgently needed at present. Analytical method for the purity of damycin B

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  • Ion chromatography detection method of gentamicin B purity
  • Ion chromatography detection method of gentamicin B purity
  • Ion chromatography detection method of gentamicin B purity

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[0034] The present invention will be described in detail below in conjunction with the implementations shown in the drawings, but it should be noted that these implementations are not limitations of the present invention, and those of ordinary skill in the art based on the functions, methods, or structural changes made by these implementations Equivalent transformations or substitutions all fall within the protection scope of the present invention.

[0035] The invention provides an ion chromatography detection method for the purity of gentamicin B. The detection method includes the following steps:

[0036] (1) Chromatographic column: using octadecylsilane bonded silica gel as filler, Agilent Polaris 5C18-A, size 4.6mm×250mm, 5μm column, column temperature 25~35℃, flow rate 0.5~1.5ml / min, The injection volume is 10-20 μl; specifically, the temperature of the chromatographic column is 30° C. The injection volume was 20 μl. The flow rate was 1.0 ml / min.

[0037] The mobile ...

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Abstract

The invention provides an ion chromatography detection method of gentamicin B purity. The chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler; the column temperature is 25-35 DEG C; the flow rate is 0.5-1.5ml / min; the injection volume is 10-20ul; and according to the method, a gold indicator electrode, a silver chloride silver reference electrodeand a stainless steel auxiliary electrode are used as pulse ampere detectors of a battery body, and the detection is respectively performed at + 0.05V, at an oxidation potential of +0.75V and a reduction potential of -0.15V according to a pulse duration used by the instrument. The ion chromatography detection method provided by the invention has the advantages of simple and rapid operation, highaccuracy and high precision, and is suitable for laboratories to detect the gentamicin B purity, and the detection can be performed in batches, so that the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to an ion chromatography detection method for the purity of gentamicin B. Background technique [0002] Gentamicin B (gentamicinb) is an alkaline water-soluble antibiotic belonging to aminoglycosides. It is produced by Micromonospora, and its antibacterial spectrum is similar to that of streptomycin, but its antibacterial activity is stronger than that of streptomycin and kanamycin. Compared with gentamicin, it has lower nephrotoxicity and ototoxicity and has resistance to some drug-resistant bacteria (such as drug-resistant bacteria with 2-acetylase inactivation); at the same time, it can be used as a raw material Synthesis of isopamicin sulfate. [0003] By consulting a large number of Chinese and foreign literature and patents, there is no literature report on the determination of the purity of gentamicin B by a method similar to ion chromatography, which is not conducive...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 华颖婷张莹钱凌燕刘显华刘倩
Owner WUXI FORTUNE PHARMA
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