Modified electrode for norfloxacin molecular imprinting electrochemical sensor and preparation method of modified electrode

A technology of molecular imprinting and modified electrodes, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of poor selectivity and achieve the effects of large specific surface area, improved sensitivity and selectivity, and fast mass transfer rate

Active Publication Date: 2019-01-25
INT ACAD OF OPTOELECTRONICS AT ZHAOQING SOUTH CHINA NORMAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of poor selectivity faced by traditional electrochemical sensing in the determination of norfloxacin, and to provide a modified electrode with molecularly imprinted polymer MWCNTs-MIP nanomaterials grafted on the surface of multi-walled carbon nanotubes with a three-dimensional network The preparation method; the MWCNTs-MIP nanomaterial modified electrode with a three-dimensional network obtained by the preparation method provided by the invention has the advantages of high porosity, large specific surface area, and good conductivity, so that the electrochemical sensor with the modified electrode has a process Simple, high sensitivity, good selectivity, low detection limit, good biocompatibility and easy to use

Method used

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  • Modified electrode for norfloxacin molecular imprinting electrochemical sensor and preparation method of modified electrode
  • Modified electrode for norfloxacin molecular imprinting electrochemical sensor and preparation method of modified electrode
  • Modified electrode for norfloxacin molecular imprinting electrochemical sensor and preparation method of modified electrode

Examples

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Effect test

Embodiment 1

[0048] Example 1 Preparation of three-dimensional network multi-walled carbon nanotube grafted molecularly imprinted polymer:

[0049] 1) Oxidative pretreatment of multi-walled carbon nanotubes.

[0050] Add 500 mg MWCNTs to 100 mL mixed acid (75 mL concentrated sulfuric acid, 25 mL concentrated nitric acid), reflux and condense at 50 ºC for 12 h; dilute the reacted dispersion, centrifuge (10000 rpm, 10 min), dialyze (7000 molecular weight dialysis bag) for 48 h, vacuum drying at 60 ºC for 24 h.

[0051] 2) Preparation of three-dimensional network of multi-walled carbon nanotubes grafted with molecularly imprinted polymers.

[0052] Put 2.5 mg of multi-walled carbon nanotubes pretreated by mixed acid oxidation into a three-necked flask, add 100 mL of anhydrous acetonitrile to disperse, and ultrasonicate for 30 min to obtain a uniformly dispersed multi-walled carbon nanotube dispersion; the methacrylic acid functional monomer ( 5mmol / L), norfloxacin template molecule (2.5 mmo...

Embodiment 2

[0066] 1) Oxidative pretreatment of multi-walled carbon nanotubes.

[0067] Reflux and condense for 24 h at 40°C; other parameters are consistent with those in Example 1.

[0068] 2) Preparation of three-dimensional network of multi-walled carbon nanotubes grafted with molecularly imprinted polymers.

[0069] Put 12.5 mg of multi-walled carbon nanotubes pretreated by mixed acid oxidation into a three-necked flask, add 100 mL of anhydrous toluene to disperse, and ultrasonicate for 30 min to obtain a uniformly dispersed multi-walled carbon nanotube dispersion; the methacrylic acid functional monomer ( 5mmol / L), norfloxacin template molecule (5 mmol / L), ethylene glycol dimethacrylate crosslinker (20 mmol / L), azobisisobutyronitrile initiator (10 mg) were added to Uniformly dispersed multi-walled carbon nanotube dispersion; after passing nitrogen and deoxygenating for 30 min, react in a 60 ºC water bath at 300 rpm, and thermally polymerize for 24 h under the protection of nitrogen...

Embodiment 3

[0075] Preparation of three-dimensional network multi-walled carbon nanotubes grafted with molecularly imprinted polymers:

[0076] 1) Oxidative pretreatment of multi-walled carbon nanotubes.

[0077] Reflux and condense for 6 h at 60 ºC; other parameters are consistent with those in Example 1.

[0078] 2) Preparation of three-dimensional network of multi-walled carbon nanotubes grafted with molecularly imprinted polymers.

[0079]Put 20.0 mg of multi-walled carbon nanotubes pretreated by mixed acid oxidation into a three-neck flask, add 100 mL of anhydrous acetonitrile to disperse, and ultrasonicate for 30 min to obtain a uniformly dispersed multi-walled carbon nanotube dispersion; the methacrylic acid functional monomer ( 5mmol / L), norfloxacin template molecules (10 mmol / L), ethylene glycol dimethacrylate cross-linking agent (25 mmol / L), azobisisobutyronitrile initiator (10 mg) were added to Uniformly dispersed multi-walled carbon nanotube dispersion; after passing nitroge...

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Abstract

The invention relates to a modified electrode for a norfloxacin molecular imprinting electrochemical sensor and a preparation method of the modified electrode. Norfloxacin is adopted as template molecules, methacrylic acid is adopted as functional monomers, ethylene glycol dimethacrylate is adopted as a cross-linking agent, a molecular imprinting polymer MWCNTs-MIP nano-modified material is grafted on the surface of a three-dimensional network multi-walled carbon nanotube prepared by means of a thermal polymerization method, and the nanotube is modified onto the surface of a substrate electrode. The electrode prepared by grafting the molecular imprinting polymer MWCNTs-MIP nano-modified material on the surface of the three-dimensional network multi-walled carbon nanotube has the advantagesof high porosity, large specific surface area, high electrical conductivity and good biocompatibility. The modified electrode is used in the electrochemical sensor and has a good detection response to norfloxacin molecules, the linear range of the modified electrode is 0.003-0.391 mirco-mol / L and 0.391-3.125 mirco-mol / L, and the detection lower limit is 1.58 nmol / L. The modified electrode can beapplied to the content detection of norfloxacin in capsule preparations and plasma samples.

Description

technical field [0001] The invention relates to a modified electrode for norfloxacin molecularly imprinted electrochemical sensor and a preparation method thereof, in particular to a preparation method and catalytic application of a three-dimensional network multi-walled carbon nanotube surface grafted with a molecularly imprinted polymer nano-modified electrode, The invention belongs to the detection technology field of electroanalytical chemistry. Background technique [0002] Norfloxacin (Norfloxacin, NFX) is a fluoroquinolone antibiotic that can effectively inhibit Chlamydia, Gram-positive bacteria, Gram-negative bacteria, Mycobacterium tuberculosis and Mycoplasma, and is widely used in human clinical and livestock Disease prevention in aquaculture. Because it can be used clinically for the treatment of various infectious diseases, there are also many adverse reactions caused by this drug, such as: colitis, abdominal pain and diarrhea, loss of appetite, and nausea and v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 水玲玲刘振平金名亮
Owner INT ACAD OF OPTOELECTRONICS AT ZHAOQING SOUTH CHINA NORMAL UNIV
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