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Virus for treating tumors

A technology of tumor and enterovirus, applied in the field of virus and tumor treatment

Active Publication Date: 2019-01-29
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of enterovirus 68 having oncolytic activity in this field

Method used

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  • Virus for treating tumors
  • Virus for treating tumors
  • Virus for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0149] Example 1: Obtainment and preparation of EV-D68 and its modified form

[0150] 1.1 Isolation of enterovirus EV-D68 from clinical samples of patients

[0151] (1) The throat swabs of the patients come from China, Xiamen Center for Disease Control and Prevention; African green monkey kidney cells (Vero cells; Number: CCL-81 TM ) is preserved by China, Xiamen University, National Engineering Technology Research Center for Diagnostic Reagents and Vaccines of Infectious Diseases, and cultured in MEM medium supplemented with 10% fetal bovine serum, glutamine, penicillin and streptomycin.

[0152] (2) Specimen processing: Fully stir the patient's throat swab in the specimen preservation solution to wash off the virus and virus-containing cells attached to the swab, and then centrifuge the specimen preservation solution at 4000rpm at 4°C for 30 minutes at high speed ;

[0153] (3) Inoculation and observation:

[0154] A. Spread Vero cells on a 24-well plate, and the numb...

Embodiment 2

[0171] Example 2: In vitro anti-tumor experiments of EV-D68 and its modified forms

[0172] 2.1 Viruses and cell lines used

[0173] (1) Viruses: EV-D68-WT (SEQ ID NO:12), EV-D68-HRV2 (SEQ ID NO:13), EV-D68-miR133&206T (SEQ ID NO:13) provided in Example 1 are used in this embodiment :14), EV-D68-GM-CSF (SEQ ID NO: 15) and EV-D68-Anti-PD-1 (SEQ ID NO: 16).

[0174] (2) Cell line: human rhabdomyosarcoma cell RD ( Number: CCL-136 TM ); human cervical cancer cell line Hela ( Number: CCL-2 TM ), SiHa( Number:HTB-35 TM ), Caski ( Number:CRL-1550 TM ) and C-33A ( Number:HTB-31 TM ); human ovarian cancer cell lines SKOV-3 / TR (SKOV-3 drug-resistant strain), SKOV-3 ( Number:HTB-77 TM ) and Caov3 ( Number:HTB-75 TM ); human endometrial cancer cell line Hec-1-A ( Number:HTB-112 TM ), Hec-1-B ( Number:HTB-113 TM ) and Ishikawa (ECACC No.99040201); human lung cancer cell line SPC-A-1 (CCTCC deposit number: GDC050), NCI-H1299 ( Number: CRL-5803 TM ), NCI-H1417...

Embodiment 3

[0214] Example 3: In vivo anti-tumor experiments of enterovirus EV-D68 and its modified forms

[0215] 3.1 Viruses, cell lines and experimental animals

[0216] (1) Viruses: EV-D68-WT (SEQ ID NO: 12), EV-D68-HRV2 (SEQ ID NO: 13), EV-D68-miR133&206T (SEQ ID NO: 13) provided by Example 1 were used in this example. NO: 14), EV-D68-GM-CSF (SEQ ID NO: 15) and EV-D68-Anti-PD-1 (SEQ ID NO: 16). Refer to Examples 2.2 and 2.3 for the methods of virus cultivation and virus titer determination, respectively.

[0217] (2) Cell line: human cervical cancer cell line Hela ( Number: CCL-2 TM ), the glioma cell line U118-MG ( Number:HTB-15 TM ), the lymphoma cell line Raji ( Number: CCL-86 TM ). Except that Raji was cultured in RPMI-1640 medium, other Hela and U118-MG were cultured in DMEM medium, and these mediums needed to be supplemented with 10% fetal bovine serum, glutamine and penicillin-streptomycin double antibody. Above all cells at 37°C, 5% CO 2 cultured under standard...

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Abstract

The invention relates to the field of virus and the field of tumor treatment. Concretely, the invention relates to enterovirus type 68 (EV-D68) or a modified form thereof, or a genomic sequence or cDNA sequence comprising the EV-D68 or the modified form of the EV-D68, or nucleic acid molecules of a complementary sequences of the genomic sequence or cDNA sequence, applications in treating tumors for a subject such as a human being, and applications in preparing pharmaceutical compositions for treating the tumors for the subject such as a human being. The invention also relates to a method for treating the tumors. The method includes steps of applying the EV-D68 or the modified form of the EV-D68, or the genomic sequence or cDNA sequence comprising the EV-D68 or the modified form of the EV-D68 or the nucleic acid molecules of the complementary sequences of the genomic sequence or cDNA sequence to the subject who needs the treatment.

Description

technical field [0001] The invention relates to the field of viruses and the field of tumor treatment. Specifically, the present invention relates to enterovirus type 68 (EV-D68) or its modified form, or the genome sequence or cDNA sequence comprising EV-D68 or its modified form, or the complementary sequence of said genome sequence or cDNA sequence Nucleic acid molecules, for use in treating tumors in a subject (eg, a human), and for the manufacture of a medicament for treating a tumor in a subject (eg, a human). The present invention also relates to a method for treating tumors, comprising administering EV-D68 or a modified form thereof, or a genome sequence or cDNA sequence comprising EV-D68 or a modified form thereof, or the genome sequence or the complement of a cDNA sequence to a nucleic acid molecule. Background technique [0002] The current treatment methods for malignant tumors mainly include surgery, chemotherapy and radiotherapy. These traditional therapies ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/765A61K48/00A61P35/00C12N7/01
CPCA61K48/005A61K35/765C12N7/00C12N2770/32321A61K48/00A61P35/00C12N15/11C12N2770/32332A61K35/768Y02A50/30A61K35/76C12N2770/32333C07K14/535C07K16/2818C07K2317/622C12N15/113C12N15/86C12N2310/141C12N2770/32343C12N2770/32371
Inventor 程通王玮万俊凯付文锟叶祥忠张军夏宁邵
Owner XIAMEN UNIV
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