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Coxsackie B virus for treatment of tumors

A coxsackie virus, tumor technology, applied in the field of virus and tumor treatment

Active Publication Date: 2019-10-29
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of CVB1 having oncolytic activity in this field

Method used

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  • Coxsackie B virus for treatment of tumors
  • Coxsackie B virus for treatment of tumors
  • Coxsackie B virus for treatment of tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0256] Example 1: Obtainment and preparation of CVB1 and its modified forms

[0257] 1.1 Isolation of enterovirus CVB1 from clinical samples of patients

[0258] (1) The patients' throat swabs and anal swabs are from China, Xiamen Center for Disease Control and Prevention; African green monkey kidney cells (Vero cells; Number: CCL-81 TM ) is preserved by China, Xiamen University, National Engineering Technology Research Center for Diagnostic Reagents and Vaccines of Infectious Diseases, and cultured in MEM medium supplemented with 10% fetal bovine serum, glutamine, penicillin and streptomycin.

[0259] (2) Specimen processing: Fully stir the patient's throat swab and anal swab in the specimen preservation solution to wash off the virus and virus-containing cells attached to the swab, and then store the specimen preservation solution at 4°C 4000rpm high-speed centrifugation for 30min;

[0260] (3) Inoculation and observation:

[0261] A. Spread Vero cells on a 24-well p...

Embodiment 2

[0278] Example 2: In vitro anti-tumor experiments of CVB1 and its modified forms

[0279] 2.1 Viruses and cell lines used

[0280] (1) Viruses: CVB1-WT (SEQ ID NO: 12), CVB1-HRV2 (SEQ ID NO: 13), CVB1-miR133&206T (SEQ ID NO: 14), CVB1- GM-CSF (SEQ ID NO: 15) and CVB1-Anti-PD-1 (SEQ ID NO: 16) and a wild-type coxsackievirus group B type 3 (hereinafter referred to as: CVB3-WT; GenBank database accession number : KY286529.1).

[0281] (2) Cell line: human rhabdomyosarcoma cell RD ( Number: CCL-136 TM ); human colorectal cancer cell line SW1116 ( Number: CCL-233 TM ), SW480 ( Number: CCL-228 TM ) and HT-29 ( Number:HTB-38 TM ); human gastric cancer cell line AGS ( Number: CRL-1739 TM ), SGC7901 (CCTCC deposit number: GDC150), BGC823 (CCTCC deposit number: GDC151) and NCI-N87 ( Number: CRL-5822 TM ); human esophageal carcinoma cell line TE-1 (purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, ...

Embodiment 3

[0327] Example 3: In vivo anti-tumor experiments of CVB1 and its modified forms

[0328] 3.1 Viruses, cell lines and experimental animals

[0329] (1) Viruses: CVB1-WT (SEQ ID NO: 12), CVB1-HRV2 (SEQ ID NO: 13), CVB1-miR133&206T (SEQ ID NO: 14), CVB1 provided in Example 1 were used in this example - GM-CSF (SEQ ID NO: 15) and CVB1-Anti-PD-1 (SEQ ID NO: 16). Refer to Examples 2.2 and 2.3 for the methods of virus cultivation and virus titer determination, respectively.

[0330] (2) Cell lines: human breast cancer cell line BcaP37 (CCTCC deposit number: GDC206), human non-small cell lung cancer cell line A549 ( Number: CCL-185 TM ) and SPC-A-1 (CCTCC deposit number: GDC050), human Burkitt's lymphoma cell line Raji ( Number: CCL-86 TM ), human endometrial cancer cell line Ishikawa (ECACCNo.99040201) and HEC-1-B ( Number:HTB-113 TM ), human cervical cancer cell line Hela ( Number: CCL-2 TM ) and C-33A ( Number:HTB-31 TM ), and the human glioma cell line GBM (t...

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Abstract

The invention relates to the field of viruses and the field of tumor therapy, in particular to coxsackie B virus 1 (CVB1) or a modified form thereof, or a nucleic acid molecule comprising a genomic sequence or a cDNA sequence comprising the CVB1 or the modified form thereof or comprising complementary sequences of the genomic sequence or the cDNA sequence, use for treatment of tumors in a subject(such as a person), and use for the preparation of a pharmaceutical composition for treatment of the tumors in the subject (such as a person). The invention further relates to a method for treatment of the tumors. The method comprises the step of applying the CVB1 or the modified form thereof, or the nucleic acid molecule comprising the genomic sequence or the cDNA sequence comprising the CVB1 orthe modified form thereof or comprising the complementary sequences of the genomic sequence or the cDNA sequence to the subject in need.

Description

technical field [0001] The invention relates to the field of viruses and the field of tumor treatment. In particular, the present invention relates to the use of CVB1 or a modified form thereof, or a nucleic acid molecule comprising the genomic nucleotide sequence of CVB1 or a modified form thereof or a complementary sequence thereof, for treating tumors in a subject (eg, a human) , and use in the manufacture of a medicament for treating a tumor in a subject (eg, a human). The present invention also relates to a method for treating tumors, comprising the step of administering CVB1 or its modified form, or a nucleic acid molecule comprising the genomic nucleotide sequence of CVB1 or its modified form or its complementary sequence to a subject in need thereof . Background technique [0002] The current treatment methods for malignant tumors mainly include surgery, chemotherapy and radiotherapy. These traditional therapies are unsatisfactory for the treatment of metastatic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N15/41A61K35/76A61P35/00
CPCC12N7/00A61K35/76C12N2770/32321C12N2770/32332A61P35/00A61P35/02A61P35/04A61K35/768C12N15/86C12N2770/32343C12N2840/203C12N2310/141Y02A50/30
Inventor 程通王玮叶祥忠付文锟潘德全张军夏宁邵
Owner XIAMEN UNIV
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