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Medium special for tissue culture and rapid propagation of gardenia jasminoides

A gardenia group and culture medium technology, applied in the direction of culture medium, planting substrate, application, etc., to achieve the effects of shortening the rooting time, increasing the content, and improving the rooting situation

Active Publication Date: 2019-02-01
江西智汇中药材种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some deficiencies in the existing medium in improving the survival rate of transplanting and the content of active ingredients, which need to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: A kind of special medium for tissue culture and rapid propagation of Gardenia jasminoides, 1 / 2MS is the basic medium, and then add 0.5mg / L accelerator, 30g / L sucrose and 7g / L agar, pH value 5.8-6.0.

[0015] The accelerator is obtained by mixing modified pine nut shells and 1-deoxynojirimycin at a mass ratio of 5:1.

[0016] The preparation method of the modified pine nut shell is as follows: crush the pine nut shell to 40 mesh, soak in the modified solution for 12 hours, filter, wash the filter residue repeatedly with distilled water until the washing liquid is neutral, and then dry it at 100°C until the water content Below 8%, that is to say.

[0017] The modified solvent is an aqueous solution of triethylenediamine and choline chloride, wherein the mass fraction of triethylenediamine is 6.5%, and the mass fraction of choline chloride is 2.5%.

Embodiment 2

[0018] Embodiment 2: A kind of special culture medium for tissue culture and rapid propagation of Gardenia jasminoides, 1 / 2MS is the basic medium, and then add 0.5mg / L accelerator, 30g / L sucrose and 7g / L agar, pH value 5.8-6.0.

[0019] The accelerator is 1-deoxynojirimycin.

Embodiment 3

[0020] Embodiment 3: a kind of special culture medium for the tissue culture and rapid propagation of Gardenia jasminoides, 1 / 2MS is the basic medium, and then add 0.5mg / L accelerator, 30g / L sucrose and 7g / L agar, the pH value 5.8-6.0.

[0021] The accelerator is a modified pine nut shell, and its preparation method is as follows: crush the pine nut shell to 40 mesh, soak it in the modified solution for 12 hours, filter, wash the filter residue repeatedly with distilled water until the washing liquid is neutral, and then bake it at 100°C Dry until the water content is below 8%.

[0022] The modified solvent is an aqueous solution of triethylenediamine and choline chloride, wherein the mass fraction of triethylenediamine is 6.5%, and the mass fraction of choline chloride is 2.5%.

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PUM

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Abstract

The invention provides a medium special for tissue culture and rapid propagation of gardenia jasminoides, and the medium can improve the rooting rate and transplanting survival rate of gardenia jasminoides. A promoter obtained by compounding of modified pine nut husks and 1-deoxynojirimycin in a specific proportion is added into a conventional rooting medium to improve the rooting rate of gardeniajasminoides, especially shorten the rooting time. Moreover, the post-transplantation survival rate can reach 100%. In addition, the invention finds that adding of the promoter at a dosage of 1.0mg / Linto an MS medium for cultivating gardenia jasminoides callus can greatly increase the content of geniposide in the callus, thus opening up a new use for the promoter provided by the invention.

Description

technical field [0001] The invention relates to a special medium for tissue culture and rapid propagation of Gardenia jasminoides, belonging to the field of rapid propagation of traditional Chinese medicine Background technique [0002] Gardenia jasminoides, also known as gardenia and gardenia jasminoides, are used as medicine with fruits and roots, and have the functions of purging fire and detoxifying, clearing away heat and dampness, cooling blood and dissipating stasis. Distributed in Zhejiang, Jiangxi, Fujian, Hubei, Sichuan, Guizhou and other provinces, Jiangxi Yichun area is mainly distributed in Zhangshu, Fengcheng, Wanzai, Fengxin and other places. [0003] The traditional breeding method of Gardenia jasminoides is division or cutting. This method has low reproduction coefficient, slow speed and long breeding cycle, which greatly hinders the cultivation and promotion of Gardenia jasminoides. The method of applying tissue culture can increase its reproduction coeffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/25A01G24/30
CPCA01G24/25A01G24/30A01H4/001
Inventor 罗志勇
Owner 江西智汇中药材种植有限公司