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Application of polypeptide and polypeptide composition in treatment of colon cancer

A polypeptide composition and colon cancer technology, applied in the field of biomedicine, can solve the problems that APC cannot be effectively combined with β-catenin, and achieve the effect of inhibiting the growth of nude mouse tumor models, inhibiting the activity of tumor cells, and having a good prospect for drug use

Pending Publication Date: 2019-02-01
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previous studies have found that because the vast majority of colon cancers are APC gene-deficient, APC cannot effectively combine with β-catenin and promote its normal degradation. Excessive β-catenin enters the nucleus to initiate the overexpression of genes related to cell growth and proliferation, causing cancer

Method used

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  • Application of polypeptide and polypeptide composition in treatment of colon cancer
  • Application of polypeptide and polypeptide composition in treatment of colon cancer
  • Application of polypeptide and polypeptide composition in treatment of colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] [Example 1] Configuration of polypeptide working solution

[0022] 1. Preparation of stock solution: 1 mg / bottle of peptide, centrifuged at 4°C, 12000 rpm, for 5 min; add PBS and 5 μl DMSO to make stock solution with a final concentration of 0.5 mM, as shown in the table below.

[0023]

[0024] 2. Preparation of working solution: Dilute the stock solution with PBS to prepare a working solution with a concentration of 5 μM.

Embodiment 2

[0025] [Example 2] The CCK-8 experiment proves the anti-colon cancer effect of the polypeptide at the cellular level

[0026] The present invention exerts its anti-tumor effect in the form of a synthetic peptide. The CCK-8 experiment proves that the polypeptide has a growth inhibitory effect on human colon cancer cells. A human colon cancer cell HT-29 is taken as an example.

[0027] 1. Cell model grouping:

[0028] (1) Normal control group: normal cells

[0029] (2) Experimental control group: cells treated with control polypeptide (SEQ ID NO.4) (100 μl of working solution for each well of 96-well plate)

[0030] (3) Experimental group 1: Cells treated with SEQ ID NO.1 polypeptide (100 μl of working solution for each well of 96-well plate)

[0031] (4) Experimental group 2: Cells treated with the polypeptide of SEQ ID NO.2 (100 μl of working solution for each well of a 96-well plate)

[0032] (5) Experimental group 3: Cells treated with the polypeptide of SEQ ID NO.3 (100 ...

Embodiment 3

[0045] [Example 3] Cell cloning experiment proves the anti-colon cancer effect of the polypeptide at the cellular level

[0046] 1. Cell culture and cell grouping are the same as in Example 2

[0047] 2. Cell cloning experiment steps:

[0048] 3. Experimental steps

[0049] (1) The treated cells of each group were digested with 0.25% trypsin and blown into single cells, and the cells were suspended in McCOY's 5A medium with 10% fetal bovine serum for use.

[0050] (2) Dilute the cell suspension by gradient multiples, and inoculate 1000 cells per dish of each group into 10 mL 37°C pre-warmed culture plates, and rotate gently to make the cells evenly dispersed. Place them in a cell culture incubator at 37°C, 5% CO2 and saturated humidity for 10-14 days.

[0051] (3) When colonies visible to the naked eye appear in the culture dish, the culture is terminated. Discard the supernatant and carefully soak twice with PBS. Crystal violet was stained for 10 min, then the staining s...

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Abstract

The invention discloses an application of a polypeptide and a polypeptide composition in the treatment of colon cancer, and belongs to the field of biomedicines. The polypeptide sequence is obtained through an antitumor therapeutic route based on the generation characteristics and treatment difficulties of tumors, and it is proved that the polypeptide has antitumor application values. The polypeptide sequence is synthesized by a biosynthesis technology, the amino acid sequences of the polypeptide are represented by SEQ ID NO.1-3, and the polypeptide composition comprises the three sequences. The antitumor function of the polypeptide and the polypeptide composition is studied, and the treatment level of the polypeptide / polypeptide composition in vivo and in vitro shows that the has the polypeptide and the polypeptide composition have great anticancer functions of inhibiting the activity of tumor cells and inhibiting the growth of a mouse tumor-forming model. The application provides anexperimental basis for the development of new targets for tumor treatment, and has a very great development and application prospect in clinical treatment of tumors.

Description

technical field [0001] The invention relates to the technical field of biomedicine, specifically, a small molecule polypeptide, a polypeptide composition and an application thereof for inhibiting tumor growth and treating colon cancer by effectively combining with β-catenin and promoting its normal degradation. Background technique [0002] Colon cancer (cancer of colon) is the most common malignant tumor in Western Europe, North America and other developed countries, and it is also one of the nine most common malignant tumors in my country. In the past 30 years, the incidence of colon cancer in most countries or regions, including my country, has been on the rise. In my country, among the deaths due to colon cancer, males rank fifth in malignant tumor deaths, and females rank sixth. From an epidemiological point of view, the incidence of colon cancer is related to social environment, lifestyle (especially eating habits, lack of physical activity), genetic factors, age, his...

Claims

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Application Information

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IPC IPC(8): C07K14/00A61K38/16A61P35/00
CPCA61P35/00C07K14/00A61K38/00
Inventor 刘红林刘海林刘星云李玥熹程牛亮牛勃覃秀桃王晓霞
Owner SHANXI MEDICAL UNIV
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