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Method of identifying ancient silk fabrics on basis of Western blots

A technology of immunoblotting and silk fabrics, applied in the field of cultural relics detection, can solve problems such as decay, unrecognizability, appearance damage, etc., achieve accurate results, high sensitivity, and avoid false positive results

Active Publication Date: 2019-02-05
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The unearthed silk fabrics are a precious heritage of our country, but as a protein material, silk has certain instability and degrades under the conditions of acid, alkali, salt, oxidizing agent, reducing agent, halogen and other conditions in the burial environment, so that the appearance So badly damaged and decayed that it cannot be seen with the naked eye
Using traditional analytical methods such as infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, etc. has certain challenges and brings certain difficulties to researchers

Method used

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  • Method of identifying ancient silk fabrics on basis of Western blots
  • Method of identifying ancient silk fabrics on basis of Western blots
  • Method of identifying ancient silk fabrics on basis of Western blots

Examples

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Embodiment 1

[0028] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0029] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 0.5wt% Na at a bath ratio of 1:100 respectively 2 CO 3Boil in the solution for degumming for 30 minutes, repeat the above operation twice, then separate the silk fibroin fibers obtained after degumming from the solution, rinse with warm water and dry at room temperature;

[0030] 2) prepare copper ethylenediamine solution, dissolve four kinds of silk fibroin fibers obtained in step 1) in copper ethylenediamine solution at 60° C. of 50 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen to oxidize 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distille...

Embodiment 2

[0044] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0045] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 1:90 bath ratio respectively in 0.6wt% Na 2 CO 3 Boil in the solution for degumming for 25 minutes, repeat the above operation 3 times, then separate the silk fiber obtained after degumming from the solution, wash it with warm water and dry it at room temperature;

[0046] 2) Copper ethylenediamine solution is prepared, and four kinds of silk fibers obtained in step 1) are dissolved in copper ethylenediamine solution at 55° C. of 45 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen oxidized 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distilled wa...

Embodiment 3

[0057] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0058] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 0.6wt% Na at a bath ratio of 1:110 respectively 2 CO 3 Boil in the solution for degumming for 35 minutes, repeat the above operation twice, then separate the silk fiber obtained after degumming from the solution, wash it with warm water and dry it at room temperature;

[0059] 2) Prepare copper ethylenediamine solution, dissolve four kinds of silk fibroin fibers obtained in step 1) in copper ethylenediamine solution at 65° C. of 55 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen to oxidize 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distilled w...

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Abstract

The invention relates to the field of cultural-relic detection, and discloses a method of identifying ancient silk fabrics on the basis of Western blots. According to the method, firstly, silk fibroins in four types of modern silkworm cocoons are extracted, then the silkworm silk fibroins are used as immunogens to prepare polyclonal antibodies and monoclonal antibodies for the silkworm silk fibroins, whether the two types of antibodies have non-specific binding to the four types of silk fibroins are detected through a Western blot method, and thus antibodies with good specificity are preparedand obtained; and then silk fibroin in an ancient silk fabric is extracted, the two types of antibodies and the Western blot method are used to detect the ancient silk fabric so as to judge whether the ancient silk fabric is a silkworm silk fabric. According to the method, on the one hand, an identification result of the ancient silk fabric is visualized, and on the other hand, difference of binding of the monoclonal antibodies and the polyclonal antibodies to samples with different regional molecular weight is clearly obtained; and the result is accurate, reliability is high, and occurrence of false positive results is also avoided at the same time.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for identifying ancient silk fabrics based on immunoblotting. Background technique [0002] As early as the Western Han Dynasty more than 2,000 years ago, China opened up the world-famous "Silk Road". Along this Silk Road, Chinese silk spread to all parts of the world. The unearthed silk fabrics are a precious heritage of our country, but as a protein material, silk has certain instability and degrades under the conditions of acid, alkali, salt, oxidizing agent, reducing agent, halogen and other conditions in the burial environment, so that the appearance So badly damaged and decayed that it cannot be seen with the naked eye. Using traditional analytical methods such as infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, etc. has certain challenges and brings certain difficulties to researchers. Contents of the invention [0003] In or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/561G01N33/577G01N33/68
CPCG01N33/561G01N33/577G01N33/68G01N33/6854G01N2550/00
Inventor 王秉陈茹茹周莲杨慧
Owner ZHEJIANG SCI-TECH UNIV