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A method for identifying ancient silk fabrics based on Western blotting

A technology of immunoblotting and silk fabrics, which is applied in the field of cultural relics detection, can solve problems such as decay, unrecognizability, and appearance damage, and achieve the effects of high sensitivity, avoiding false positive results, and easy experimental operation

Active Publication Date: 2021-06-25
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The unearthed silk fabrics are a precious heritage of our country, but as a protein material, silk has certain instability and degrades under the conditions of acid, alkali, salt, oxidizing agent, reducing agent, halogen and other conditions in the burial environment, so that the appearance So badly damaged and decayed that it cannot be seen with the naked eye
Using traditional analytical methods such as infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, etc. has certain challenges and brings certain difficulties to researchers

Method used

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  • A method for identifying ancient silk fabrics based on Western blotting
  • A method for identifying ancient silk fabrics based on Western blotting
  • A method for identifying ancient silk fabrics based on Western blotting

Examples

Experimental program
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Embodiment 1

[0028] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0029] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 0.5wt% Na at a bath ratio of 1:100 respectively 2 CO 3Boil in the solution for degumming for 30 minutes, repeat the above operation twice, then separate the silk fibroin fibers obtained after degumming from the solution, rinse with warm water and dry at room temperature;

[0030] 2) prepare copper ethylenediamine solution, dissolve four kinds of silk fibroin fibers obtained in step 1) in copper ethylenediamine solution at 60° C. of 50 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen to oxidize 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distille...

Embodiment 2

[0044] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0045] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 1:90 bath ratio respectively in 0.6wt% Na 2 CO 3 Boil in the solution for degumming for 25 minutes, repeat the above operation 3 times, then separate the silk fiber obtained after degumming from the solution, wash it with warm water and dry it at room temperature;

[0046] 2) Copper ethylenediamine solution is prepared, and four kinds of silk fibers obtained in step 1) are dissolved in copper ethylenediamine solution at 55° C. of 45 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen oxidized 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distilled wa...

Embodiment 3

[0057] A method for identifying ancient silk fabrics based on western blotting, comprising the steps of:

[0058] 1) Take modern mulberry silkworm cocoons, tussah silkworm cocoons, castor-bean silkworm cocoons and chestnut silkworm cocoons respectively, and use 0.6wt% Na at a bath ratio of 1:110 respectively 2 CO 3 Boil in the solution for degumming for 35 minutes, repeat the above operation twice, then separate the silk fiber obtained after degumming from the solution, wash it with warm water and dry it at room temperature;

[0059] 2) Prepare copper ethylenediamine solution, dissolve four kinds of silk fibroin fibers obtained in step 1) in copper ethylenediamine solution at 65° C. of 55 times its mass respectively (the preparation method of copper ethylenediamine solution is: take hydrogen to oxidize 6.6 grams of copper, add 30 milliliters of distilled water, after dissolving, add 8.5 milliliters of 9wt% ethylenediamine, after fully stirring to be dissolved, add distilled w...

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Abstract

The invention relates to the field of detection of cultural relics, and discloses a method for identifying ancient silk fabrics based on immunoblotting. The invention first extracts silk fibroin from four modern silkworm cocoons, and then uses the silk fibroin as an immunogen to prepare a method for targeting silk fibroin. The polyclonal antibody and monoclonal antibody of the protein were tested by immunoblotting to detect whether the two antibodies had non-specific binding to the four silk fibroin proteins, so as to prepare antibodies with good specificity. Then, the silk fibroin in the ancient silk fabrics was extracted, and two kinds of antibodies were used to detect the ancient silk fabrics by immunoblotting to determine whether the ancient silk fabrics were mulberry silk fabrics. On the one hand, the present invention visualizes the identification results of ancient silk fabrics; on the other hand, it clearly obtains the difference in the molecular weight binding of monoclonal antibodies and polyclonal antibodies to the sample region, and the results are accurate and reliable, and at the same time avoid the risk of false positive results. Appear.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for identifying ancient silk fabrics based on immunoblotting. Background technique [0002] As early as the Western Han Dynasty more than 2,000 years ago, China opened up the world-famous "Silk Road". Along this Silk Road, Chinese silk spread to all parts of the world. The unearthed silk fabrics are a precious heritage of our country, but as a protein material, silk has certain instability and degrades under the conditions of acid, alkali, salt, oxidizing agent, reducing agent, halogen and other conditions in the burial environment, so that the appearance So badly damaged and decayed that it cannot be seen with the naked eye. Using traditional analytical methods such as infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, etc. has certain challenges and brings certain difficulties to researchers. Contents of the invention [0003] In or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/561G01N33/577G01N33/68
CPCG01N33/561G01N33/577G01N33/68G01N33/6854G01N2550/00
Inventor 王秉陈茹茹周莲杨慧
Owner ZHEJIANG SCI-TECH UNIV