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Application of NK cell combined with mesenchymal stem cell in treatment of hepatitis and liver fibrosis

A technology of mesenchymal stem cells and NK cells, applied in the field of stem cell applications

Inactive Publication Date: 2019-02-12
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the combination of NK cells and mesenchymal stem cells for the treatment of hepatitis and liver fibrosis

Method used

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  • Application of NK cell combined with mesenchymal stem cell in treatment of hepatitis and liver fibrosis
  • Application of NK cell combined with mesenchymal stem cell in treatment of hepatitis and liver fibrosis
  • Application of NK cell combined with mesenchymal stem cell in treatment of hepatitis and liver fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A mixed cell of NK cells and mesenchymal stem cells, wherein the number ratio of NK cells and mesenchymal stem cells is 1:4.

Embodiment 2

[0028] A mixed cell of NK cells and mesenchymal stem cells, wherein the number ratio of NK cells and mesenchymal stem cells is 1:5.

Embodiment 3

[0030] A mixed cell of NK cells and mesenchymal stem cells, wherein the number ratio of NK cells and mesenchymal stem cells is 1:6;

[0031] The NK cells and the mesenchymal stem cells are all cultured separately and then mixed;

[0032] The specific method for cultivating the NK cells is: culturing the NK cells in the first culture medium, and when the cultured cell density reaches 3×10 7 cells / mL, add the second culture medium for subculture, and collect the fourth-generation mesenchymal stem cells; the specific method for culturing the mesenchymal stem cells is: culture the mesenchymal stem cells in the third culture medium, when cultured After the confluence of the cells reached 85%, the second medium was added for subculture, and the fourth generation mesenchymal stem cells were collected;

[0033] Described first culture medium is made up of DMEM culture fluid and first additive, and each component of described first additive and its concentration in DMEM culture fluid ...

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PUM

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Abstract

The invention provides an NK cell combined with a mesenchymal stem cell, and application of a composition and an injection thereof in preparation of drugs for treating hepatitis and liver fibrosis. The NK cell combined with the mesenchymal stem cell and the composition and the injection thereof can be used for treating hepatitis B, can reduce liver indexes, activity of ALT and AST in serum and MDAcontent in liver tissue, and has the function of enhancing SOD activity. Therefore, the NK cell combined with the mesenchymal stem cell and the composition and the injection thereof provided by the invention can also be used for treating liver fibrosis.

Description

technical field [0001] The invention belongs to the field of stem cell applications, and in particular relates to an application of NK cells combined with mesenchymal stem cells in the treatment of hepatitis and liver fibrosis. Background technique [0002] As one of the largest and most functional substantive glandular organs in the human body, the liver participates in many processes in the human body, such as in vivo digestion, metabolism, bile excretion, detoxification, and immunity. With the development of the economy, liver function damage is related to The incidence of disease is increasing year by year. Studies have shown that liver lesions present a trend of diversification and refractory. At present, the treatment methods for liver diseases mainly include etiological treatment, diet therapy and drug therapy, and there is no very satisfactory drug so far. Finding a safe and effective drug with less side effects for the treatment of liver damage has become a clinic...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K36/899A61K9/08A61P1/16A61P31/20A61K35/17
CPCA61K9/0019A61K9/08A61K35/17A61K35/28A61K36/535A61K36/56A61K36/73A61K36/899A61P1/16A61P31/20A61K2300/00
Inventor 刘世红董明珠潘新卢承前
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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