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Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method

A technology of compound enzymatic method and compound enzymatic hydrolysis, applied in the field of extraction of yellow ginseng polysaccharide, can solve problems such as large organ damage

Active Publication Date: 2014-10-01
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs take effect quickly, they cause great damage to organs such as liver and kidney.

Method used

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  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method
  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method
  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:30g / mL, adjust the pH value to 5, and set the temperature at 60°C , microwave power is 200W, microwave extraction 10min;

[0023] (2) Double-enzyme extraction: adjust the stirring speed to 1200r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 4 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) / g) Hydrolyze for 4 hours, inactivate the enzyme at 90°C for 10 minutes, and cool to room temperature to obtain the extract;

[0024] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 10 minutes, take the supernatant, and filter twice with double-layer filter paper, at a pressure of 0.07Mpa, concentrate under reduced pressure at 60°C until the volume is 1% of the volume of the extract. / 5, ad...

Embodiment 2

[0028] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:34g / mL, adjust the pH value to 5.5, and set the temperature at 55°C , microwave power is 220W, microwave extraction 8min;

[0029] (2) Double-enzyme extraction: adjust the stirring speed to 800r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 3.5 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) was hydrolyzed for 3.5 hours, the enzyme was inactivated at 92°C for 12 minutes, and cooled to room temperature to obtain the extract;

[0030] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 12 minutes, take the supernatant, and filter twice with double-layer filter paper under a pressure of 0.08Mpa. Concentrate under reduced pressure at 65°C until the volume is 1% of the volume of the ex...

Embodiment 3

[0034] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:38g / mL, adjust the pH value to 6.5, and set the temperature at 58°C , the microwave power is 250W, and the microwave extraction is 12min;

[0035] (2) Double-enzyme extraction: adjust the stirring speed to 1000r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 3 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) / g) Hydrolyze for 3 hours, inactivate the enzyme at 94°C for 15 minutes, and cool to room temperature to obtain the extract;

[0036] (3) Concentration and precipitation: Centrifuge the extract at 4000r / min for 15min, take the supernatant, and filter twice with double-layer filter paper, at a pressure of 0.09Mpa, concentrate under reduced pressure at 70°C until the volume is 1 / of the volume of the extra...

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Abstract

The invention discloses a process for extracting sphallerocarpus gracilis polysaccharide through a complex enzyme method. The process comprises the following steps: drying and grinding sphallerocarpus gracilis, adding water, implementing microwave extraction, hydrolyzing through cellulase and hydrolyzing through papain, conducting enzyme deactivation, cooling, centrifuging and reserving a supernatant liquid, extracting through two layers of filter paper, decompressing and concentrating, precipitating through ethanol, and freeze-drying to obtain the sphallerocarpus gracilis polysaccharide, wherein polysaccharide yield is 18.92-21.35%. Through a phenol-sulfuric acid method, polysaccharide content in an extract is detected to be 88.33%+ / -0.93%, and through coomassie brilliant blue, protein content is tested to be 2.17%+ / -1.03%. Experiments show that the sphallerocarpus gracilis polysaccharide has a remarkable inhibiting effect on blood sugar and blood fat of experimental hyperglycemic rats, and is safe and free from side effect. After being ground, the freeze-dried extract is prepared into tablets, hard capsules, granules and the like; and the sphallerocarpus gracilis polysaccharide is a natural healthcare product having a function of regulating blood sugar and blood fat.

Description

technical field [0001] The invention relates to a method for extracting yellow ginseng polysaccharides, in particular to a method for extracting yellow ginseng polysaccharides by a compound enzyme method. Background technique [0002] With the acceleration of the pace of life in today's society and the increase in life pressure, the number of people suffering from hypertension, hyperglycemia, and hyperlipidemia (also known as the three highs) is increasing day by day. Nowadays, the prevention and treatment of the three highs mainly depends on western medicine, such as Captopril Tablets, Compound Jiangya Tablets, Qingnao Jiangya Tablets, etc. for the prevention and treatment of high blood pressure. Drugs for hyperlipidemia such as simvastatin, Pragut, etc. Although these medicines take effect quickly, they cause greater damage to organs such as the liver and kidneys. The pure natural plant extracts have the advantages of mild action, no toxic side effects, etc., and have gr...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张继宋珅程昉殷振雄刘琴赵保堂丁昊安珂付亚娟
Owner NORTHWEST NORMAL UNIVERSITY
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