Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method

A technology of compound enzymatic method and compound enzymatic hydrolysis, applied in the field of extraction of yellow ginseng polysaccharide, can solve problems such as large organ damage

Active Publication Date: 2014-10-01
NORTHWEST NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these drugs take effect quickly, they cau

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  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method
  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method
  • Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method

Examples

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Example Embodiment

[0021] Example 1

[0022] (1) Microwave extraction: Take perennial yellow ginseng, dry at 60°C for 12 hours, pulverize it with a grinder, and pass through a 60-mesh sieve; add water according to the material-to-liquid ratio of 1:30g / mL, adjust pH=5, and set the temperature to 60°C , The microwave power is 200W, and the microwave extraction is 10min;

[0023] (2) Double enzyme extraction: adjust the stirring speed to 1200r / min, first hydrolyze with 1.5% yellow ginseng cellulase (50,000 U / g) for 4 hours, and then use 0.3% yellow ginseng papain (65 million U / g). / g) Hydrolyze for 4h, inactivate the enzyme at 90°C for 10min, cool to room temperature to obtain the extract;

[0024] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 10 minutes, take the supernatant, and filter twice with double-layer filter paper at a pressure of 0.07Mpa. Concentrate under reduced pressure at 60°C to a volume of 1 of the volume of the extract. / 5, add 95% ethanol whose volume i...

Example Embodiment

[0027] Example 2

[0028] (1) Microwave extraction: take perennial yellow ginseng, dry at 60°C for 12 hours, pulverize with a grinder, and pass through a 60-mesh sieve; add water according to a material-to-liquid ratio of 1:34g / mL, adjust pH=5.5, set temperature to 55°C , The microwave power is 220W, and the microwave extraction is 8min;

[0029] (2) Double enzyme extraction: adjust the stirring speed to 800r / min, first hydrolyze with 1.5% yellow ginseng cellulase (50,000 U / g) for 3.5 hours, and then use 0.3% yellow ginseng papain (65 million U / g). U / g) hydrolyze for 3.5h, inactivate the enzyme at 92°C for 12min, cool to room temperature to obtain the extract;

[0030] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 12 minutes, take the supernatant, and filter twice with double-layer filter paper at a pressure of 0.08Mpa. Concentrate under reduced pressure at 65°C to a volume of 1 of the volume of the extract. / 5, add 95% ethanol whose volume is 3 time...

Example Embodiment

[0033] Example 3

[0034] (1) Microwave extraction: Take perennial yellow ginseng, dry at 60°C for 12 hours, pulverize it with a grinder, and pass through a 60-mesh sieve; add water at a material-to-liquid ratio of 1:38g / mL, adjust pH=6.5, set temperature to 58°C , The microwave power is 250W, and the microwave extraction is 12min;

[0035] (2) Double enzyme extraction: adjust the stirring speed to 1000r / min, first hydrolyze with 1.5% yellow ginseng cellulase (50,000 U / g) for 3 hours, and then use 0.3% yellow ginseng papain (650,000 U / g). / g) Hydrolyze for 3h, inactivate the enzyme at 94°C for 15min, cool to room temperature to obtain the extract;

[0036] (3) Concentration and precipitation: Centrifuge the extract at 4000r / min for 15min, take the supernatant, and filter twice with double-layer filter paper at a pressure of 0.09Mpa and concentrate under reduced pressure at 70°C until the volume is 1 / the volume of the extract. 4. Add 95% ethanol with a volume of 4 times the volume o...

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Abstract

The invention discloses a process for extracting sphallerocarpus gracilis polysaccharide through a complex enzyme method. The process comprises the following steps: drying and grinding sphallerocarpus gracilis, adding water, implementing microwave extraction, hydrolyzing through cellulase and hydrolyzing through papain, conducting enzyme deactivation, cooling, centrifuging and reserving a supernatant liquid, extracting through two layers of filter paper, decompressing and concentrating, precipitating through ethanol, and freeze-drying to obtain the sphallerocarpus gracilis polysaccharide, wherein polysaccharide yield is 18.92-21.35%. Through a phenol-sulfuric acid method, polysaccharide content in an extract is detected to be 88.33%+/-0.93%, and through coomassie brilliant blue, protein content is tested to be 2.17%+/-1.03%. Experiments show that the sphallerocarpus gracilis polysaccharide has a remarkable inhibiting effect on blood sugar and blood fat of experimental hyperglycemic rats, and is safe and free from side effect. After being ground, the freeze-dried extract is prepared into tablets, hard capsules, granules and the like; and the sphallerocarpus gracilis polysaccharide is a natural healthcare product having a function of regulating blood sugar and blood fat.

Description

technical field [0001] The invention relates to a method for extracting yellow ginseng polysaccharides, in particular to a method for extracting yellow ginseng polysaccharides by a compound enzyme method. Background technique [0002] With the acceleration of the pace of life in today's society and the increase in life pressure, the number of people suffering from hypertension, hyperglycemia, and hyperlipidemia (also known as the three highs) is increasing day by day. Nowadays, the prevention and treatment of the three highs mainly depends on western medicine, such as Captopril Tablets, Compound Jiangya Tablets, Qingnao Jiangya Tablets, etc. for the prevention and treatment of high blood pressure. Drugs for hyperlipidemia such as simvastatin, Pragut, etc. Although these medicines take effect quickly, they cause greater damage to organs such as the liver and kidneys. The pure natural plant extracts have the advantages of mild action, no toxic side effects, etc., and have gr...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张继宋珅程昉殷振雄刘琴赵保堂丁昊安珂付亚娟
Owner NORTHWEST NORMAL UNIVERSITY
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