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Rainbow trout breeding method for environment release detection of nucleic acid vaccine

A nucleic acid vaccine and breeding method technology, which is applied in the field of rainbow trout breeding for nucleic acid vaccine environmental release detection, can solve the problem of insufficient research, insufficient research on immune system immune response laws and protection mechanisms, and restrict the development of genetically engineered seedlings or nucleic acid vaccines, etc. problem, to achieve the effect of stable water quality indicators

Inactive Publication Date: 2019-02-12
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current research on fish vaccines has the following deficiencies: First, the basic research on fish immunity is relatively weak, and the research on the immune response rules and protection mechanisms of the immune system is not deep enough; second, the pathogenic bacteria of many major diseases have not been isolated, and it is impossible to prepare whole bacteria Inactivated vaccines; some pathogenic bacteria that have been isolated have limited the development of genetic engineering vaccines or nucleic acid vaccines due to insufficient research on their follow-up tests

Method used

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  • Rainbow trout breeding method for environment release detection of nucleic acid vaccine
  • Rainbow trout breeding method for environment release detection of nucleic acid vaccine
  • Rainbow trout breeding method for environment release detection of nucleic acid vaccine

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specific Embodiment approach 1

[0029] A method for breeding rainbow trout for nucleic acid vaccine environmental release detection, comprising the steps of:

[0030] Step 1. Before the environmental release aquaculture test, the rainbow trout aquaculture system used for nucleic acid vaccine environmental release detection will be in normal operation for 25 to 30 days, and the operating parameters and water body indicators of the detection system will be used after passing the test;

[0031] Step 2. Select rainbow trout with a size of 4-6g / tail. After fasting for 2 days, perform intramuscular injection of nucleic acid vaccine at the base of the dorsal fin. During the immunization, randomly divide the rainbow trout into a blank group and an immunization group. After injection, put the rainbow trout into In the culture pond of the rainbow trout culture system used for nucleic acid vaccine environmental release detection;

[0032] Step 3. The total water volume of the rainbow trout culture system used for nucle...

specific Embodiment approach 2

[0044] A rainbow trout culture method for nucleic acid vaccine environmental release detection, comprising the following steps:

[0045] Step 1. Before the environmental release culture test, run the rainbow trout culture system used for nucleic acid vaccine environmental release detection normally for 25 to 30 days, test the system operating parameters and water body indicators, and stand by after passing the test;

[0046] Step 2. Select rainbow trout with a size of 4-6 g / tail. After fasting for 2 days, carry out immunization by intramuscular injection of nucleic acid vaccine at the base of the dorsal fin. During immunization, rainbow trout are randomly divided into blank group and immune group. In the breeding tank of the rainbow trout breeding system used for nucleic acid vaccine environmental release detection;

[0047] Step 3. The total water volume of the rainbow trout breeding system used for nucleic acid vaccine environmental release detection is 10m 3 , the daily wa...

specific Embodiment approach 3

[0049] According to the rainbow trout culture method for nucleic acid vaccine environmental release detection according to the second embodiment, the biological filter device described in step 1 is a stainless steel shell, which is divided into a biological filter layer and a ceramic filler layer from top to bottom. , three-dimensional elastic filter material layer, activated carbon biological filler layer, the biological filter layer is EPS biological balls with a height of 30-50cm, the thickness of the ceramic particles is 40-60cm, and the particle size of the ceramic particles is 4-6mm, The thickness of the three-dimensional elastic filter material layer is 30cm, the three-dimensional elastic filter material layer is made of polyethylene, the diameter of the elastic filter material unit is 80mm, and the specific surface area is 300m 2 / m 3 , the porosity is greater than 99%, the two sides of the filler are fixed with stainless steel mesh to prevent the filler from flutterin...

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Abstract

The invention relates to a rainbow trout breeding method for environment release detection of a nucleic acid vaccine, belongs to the technical field of rainbow trout environment release detection, andsolves the problem of subsequent experiment research shortage of fishery vaccines. Before an environment release breeding test, a rainbow trout breeding system for environment release detection afterinjection of the nucleic acid vaccine normally runs for 25-30 days; system operation parameters and water body indexes are detected; rainbow trout with individual weight of 4-6g is selected after thedetection is passed; dorsal fin base muscle injection immunization of the nucleic acid vaccine is performed in 2 days of stopping eating; the rainbow trout is randomly divided into a blank group andan immune group during the immunization; after the injection, the rainbow trout is placed in a breeding pond for breeding in the rainbow trout breeding system for the environment release detection after the injection of the nucleic acid vaccine; and the environment release detection is carried out at a certain time interval. The method can ensure that a breeding environment is suitable after the vaccine is injected.

Description

technical field [0001] The invention belongs to the technical field of rainbow trout environmental release detection, and in particular relates to a rainbow trout breeding method for nucleic acid vaccine environmental release detection. Background technique [0002] In the past 30 years, the incidence of aquatic diseases has increased rapidly and some unknown diseases have caused harm to the aquaculture industry. In order to prevent the spread of these infectious diseases, aquaculture uses a large number of antibiotics to prevent and treat fish diseases, resulting in many serious sequelae. Moreover, the repeated use of antibiotics has produced a large number of drug-resistant strains, so the dosage of antibiotics has to be continuously increased, and a vicious cycle has resulted in more drug-resistant strains. Therefore, in order to reduce the incidence of aquatic diseases, in recent years, the aquaculture industry has also begun to advocate the implementation of healthy fa...

Claims

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Application Information

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IPC IPC(8): G01N33/18A01K61/13
CPCA01K61/13G01N33/186Y02A40/81
Inventor 卢彤岩徐黎明赵景壮韩世成周涛
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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