Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Isolation medium for lactic acid bacteria and preparation method of isolation medium as well as lactic acid bacterium screening method

A technology for separating culture medium and lactic acid bacteria, applied in the direction of separating microorganisms, bacteria, fungi, etc., can solve the problems of adding Daqu-wine grain extract, etc., and achieve the effect of promoting separation and overcoming the deepening of background color.

Active Publication Date: 2019-02-15
KWEICHOW MOUTAI COMPANY
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, with regard to the isolation and screening of lactic acid bacteria in Maotai-flavored fermented grains, there has been no report on the addition of Daqu-distilled grains extract to the medium, and the isolation and screening of lactic acid bacteria without high-throughput screening technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isolation medium for lactic acid bacteria and preparation method of isolation medium as well as lactic acid bacterium screening method
  • Isolation medium for lactic acid bacteria and preparation method of isolation medium as well as lactic acid bacterium screening method
  • Isolation medium for lactic acid bacteria and preparation method of isolation medium as well as lactic acid bacterium screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1 Lactic acid bacteria isolation medium formula and preparation method thereof

[0070] Components and contents of the medium in table 1

[0071]

[0072]

[0073] The preparation method of Daqu-wine grain extract is as follows:

[0074] (1) According to the Daqu-wine grains ratio and content shown in Table 1, weigh the wine grains and Daqu, and mix;

[0075] (2) Add pure water to the mixture of Daqu-wine grains and boil for 10-20 minutes;

[0076] (3) After cooling, filter with 4 layers of gauze to obtain the filtrate for subsequent use;

[0077] (4) The above filtrate was frozen at -20°C overnight and then thawed out, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was taken for later use.

[0078] Lactic acid bacteria isolation medium preparation method:

[0079] (1) Weigh each component according to the formula in Table 1. Peptone, beef extract, yeast powder, glucose, dipotassium hydrogen phosphate, diammonium hydrogen citrate, ...

Embodiment 2

[0082] Embodiment 2 medium of the present invention is to lactic acid bacteria growth and screening effect evaluation

[0083] In order to evaluate the influence of the culture medium of the present invention on the growth of lactic acid bacteria in fermented grains and the evaluation of the screening effect of lactic acid bacteria in fermented fermented grains, the present invention adopts the plate dilution method to dilute the fermented fermented grains bacterium liquid to 10 -2 ~10 -3 , coated on each group of medium in Example 1 for anaerobic culture, the culture time is 5-7 days. Choose a dilution of 10 -2 Lactic acid bacteria growth plate is compared, and growth situation is as table 2, figure 1 shown.

[0084] These culture plates inoculated with lactic acid bacteria were identified and selected using the high-throughput screening instrument Q-Pix. The test results are shown in Table 2.

[0085] Among them, 1, 2, 3, 4, 5, 6, 7, and 8 are used to represent the dept...

Embodiment 3

[0092] Effects of different ratios of Daqu and fermented grains on the growth of lactic acid bacteria and the screening effect in the medium of embodiment 3

[0093] In order to test the influence of different ratios of Daqu and fermented grains on the growth of lactic acid bacteria, the lactic acid bacteria from fermented fermented grains were spread on the culture medium containing the ratio of Daqu: fermented grains extract in Table 3 by plate dilution method, and cultured in each group. The remaining components and contents of the culture medium and other conditions are the same as those of the culture medium 2 in Example 1. The results are shown in Table 3. Wherein culture time and lactic acid bacteria dilution concentration are identical with embodiment 2.

[0094] The high-throughput screening instrument Q-Pix was used to identify and select these culture plates inoculated with lactic acid bacteria. The test results were as follows: figure 2 shown.

[0095] Among th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention relates to an isolation medium for lactic acid bacteria in the technical field of Chinese liquor brewing, in particular to an isolation medium for lactic acid bacteria in fermented grains and a preparation method of the isolation medium as well as a lactic acid bacterium screening method. The isolation medium for the lactic acid bacteria in the fermented grains is characterized in that a formula of the medium comprises 25-100mL / L of a Daqu-fermented grain extraction solution. Through centrifugation of the Daqu-fermented grain extraction solution in the medium, the defects that the medium is deepened in background color and contains a large number of impurities can be overcome; bacterial colony identification and screening can be carried out on a manufactured culture plate through the high-throughput screening technology, so that the requirements for growth, isolation and high throughput screening of the lactic acid bacteria in the fermented grains can be simultaneously satisfied; and the working efficiency is much higher than that of manual selection.

Description

technical field [0001] The invention relates to a lactic acid bacteria isolation medium in the technical field of liquor brewing, in particular to a lactic acid bacteria isolation medium in fermented grains, a preparation method thereof and a lactic acid bacteria screening method. Background technique [0002] Liquor production is an open solid-state fermentation process, and the composition of the microbial community in the fermented grains is very complex. After long-term domestication in brewing activities, the microorganisms in the fermented grains can grow and metabolize better in the environment of high acidity and diverse nutrients in the fermented grains. [0003] Lactic acid bacteria is a general term for a class of microorganisms that can ferment sugars to produce lactic acid. They are commonly found in the fermentation process of liquor and have an important impact on the quality of liquor. An in-depth study of the acid-producing metabolic mechanism of different t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/16
CPCC12N1/02C12N1/16C12N1/20
Inventor 杨玉波杨帆袁颉张巧玲吴耀领王和玉王莉白逢彦韩培杰刘延峰
Owner KWEICHOW MOUTAI COMPANY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com