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Preservation solution for fluorescent microspheres and preparation method thereof

A technology of fluorescent microspheres and preservation solutions, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, and can solve problems such as poor stability and poor preservation effect of fluorescent microspheres

Active Publication Date: 2021-12-21
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing fluorescent microspheres have the advantages of uniform particle size, good dispersion, and high luminous efficiency, but their stability is poor and they will be affected by the external environment and media
Usually, the fluorescent microspheres need to be preserved in the preservation solution. At present, the preservation solution mainly includes buffer and preservatives, etc., and the preservation effect on the fluorescent microspheres is not good.

Method used

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  • Preservation solution for fluorescent microspheres and preparation method thereof
  • Preservation solution for fluorescent microspheres and preparation method thereof
  • Preservation solution for fluorescent microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Raw material: ethanol 4%, citric acid-sodium citrate buffer (0.1M) 2.38%, sodium chloride 0.1%, sucrose 1.0%, PEG-20000 0.1%, deionized water balance.

[0047] Preparation: Dissolve sodium chloride, sucrose, and buffer solution in partially deionized water, then add ethanol, adjust the pH to 4.0, and dilute to volume with the remaining deionized water to obtain a preservation solution.

Embodiment 2

[0049] Take 100mL fluorescent microspheres (solid content is 2%, model is F8809, provided by ThermoFisher Technology Co., Ltd.), and divided into two parts, each 50mL. Take one part and transfer it to a centrifuge tube, remove the supernatant after centrifugation, add 50mL of the preservation solution in Example 1, shake on the oscillator for 3-5min, and then ultrasonicate for 5-10min, so that the fluorescent microspheres are evenly dispersed in the preservation solution. In the liquid, it is recorded as the sample. Another fluorescent microsphere without preservation solution was used as a blank control sample.

[0050] The above two samples were stored at 37°C, and the accelerated stability tests of fluorescence signal and biological activity were carried out (accelerated condition: 37°C), and the test time points were 0 day, 20 days, 40 days, and 60 days, respectively.

[0051] (1) The test of fluorescence signal stability is as follows:

[0052] Take 1mL samples from the...

Embodiment 3

[0065] Take 50mL fluorescent microspheres (solid content is 2%, model is F8809, provided by ThermoFisher Technology Co., Ltd.), transfer to a centrifuge tube, remove the supernatant after centrifugation, add 50mL of the preservation solution in Example 1, and vibrate on a shaker 3 to 5 minutes, and then ultrasonic treatment for 5 to 10 minutes, so that the fluorescent microspheres are evenly dispersed in the preservation solution, and recorded as the sample.

[0066] The above samples were stored at room temperature (25°C), and the real-time stability tests of fluorescence signal and biological activity were carried out (real-time conditions were 25°C), and the test time points were 0 month, 6 months, 12 months, and 24 months, respectively. months, 36 months.

[0067] (1) The test of fluorescence signal stability is as follows:

[0068] Take 1mL sample with a pipette gun, centrifuge to remove the supernatant, then add 1mL deionized water, shake on the oscillator for 3-5min, a...

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Abstract

The invention provides a preservation solution of fluorescent microspheres and a preparation method thereof. The preservation solution provided by the present invention comprises the following components by mass ratio: 0.8% to 8% ethanol, 1.2% to 4.8% buffer, 0.05% to 0.15% inorganic sodium salt, 0.5% to 1.5% carbohydrates, 0.05% to 0.15% organic dispersant and the rest water. In the preservation solution provided by the present invention, ethanol, sugars and organic dispersants act synergistically to enhance the protective effect on the surface active groups of fluorescent microsphere particles. At the same time, the ion concentration of the system and the buffering of the buffer are adjusted by inorganic sodium salts It has a better protective effect on fluorescent microspheres, and significantly improves the stability of its fluorescent signal and biological activity.

Description

technical field [0001] The invention relates to the technical field of biochemical preparations, in particular to a preservation solution of fluorescent microspheres and a preparation method thereof. Background technique [0002] Fluorescent microspheres are a kind of functional microspheres. Specifically, fluorescent substances are marked on the surface of the microspheres through chemical reaction or physical adsorption, or a certain fluorescent substance is embedded or polymerized inside the microspheres, so that the microspheres have a fluorescent display effect. ; Fluorescent microspheres can be in any shape, mainly spherical. At present, the types of fluorescent microspheres include quantum dot fluorescent microspheres, rare earth fluorescent microspheres, self-luminous fluorescent microspheres and so on. Because fluorescent microspheres emit fluorescence after being stimulated by external energy, they are widely used in biomedical fields such as fluorescence immunoas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02G01N21/64
CPCG01N21/64C09K11/025
Inventor 魏艳芬李宗祥
Owner SINOCARE