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Method for preparing anti-biological inoculant of Trichoderma atroviride from stevia rebaudiana residues

A biocontrol agent, the technology of Trichoderma dark green, applied in the direction of using spores, microorganism-based methods, botanical equipment and methods, etc., to achieve the effect of high fermentation unit, promoting crop growth, and increasing soil fertility

Inactive Publication Date: 2019-02-22
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And only with stevia rebaudiana slag as solid medium large-scale solid fermentation Trichoderma dark green method has not been reported at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The strain used is a strain of Trichoderma atroviride ZYTA09 preserved in the Microbiology Laboratory of Shandong Agricultural University. . The bacterial strain has a strong antagonistic effect on various plant pathogenic fungi, and also has a certain control effect on plant root-knot nematodes. Trichoderma dark green can produce a variety of enzymes with biological activity, and it is one of the strains with the highest cellulase activity. The cellulase produced has a degrading effect on crops, and the effect is very good. It is also a resource Abundant antagonistic microorganisms play an important role in the biological control of plant pathology, have dual functions of protection and treatment, and can effectively prevent and control soil-borne diseases.

[0039] (1) Stevia residue treatment: dry the fresh stevia residue at 121° C. for 12 hours, pulverize to a particle size of 0.2-0.6 cm, and set aside;

[0040] (2) Seed culture of Trichoderma dark viridans strain...

Embodiment 2

[0048]Utilizing stevia rebaudiana slag to produce dark green dark green Trichoderma biocontrol agent

[0049] (1) Stevia residue treatment: dry the fresh stevia residue at 121° C. for 12 hours, pulverize to a particle size of 0.2-0.6 cm, and set aside;

[0050] (2) Seed culture of Trichoderma dark viridans strain

[0051] Trichoderma dark viride seeds were inoculated on potato dextrose agar medium, the culture temperature was 28°C, the light conditions were 16 hours of light and 8 hours of darkness alternately, and the culture time was 4 days;

[0052] (3) Preparation of Trichoderma dark green fermented liquid

[0053] Pick a small amount of mycelia from the Trichoderma dark green cultured in step (2), add it to the PDB medium, place it in a shaker at 25-30°C and cultivate it at 180r / min, and cultivate it continuously for 3-5 days to obtain dark green Trichoderma liquid fermentation broth;

[0054] (4) Trichoderma dark green fermented liquid is added in stevia rebaudiana sl...

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PUM

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Abstract

The invention provides a method for preparing an anti-biological inoculant of Trichoderma atroviride from stevia rebaudiana residues. A Trichoderma atroviride ZYTA09 strain is collected with the collection number CGMCC 14133 in China general microbiological culture collection center. The anti-biological inoculant is prepared by fermenting Trichoderma atroviride ZYTA09 by using the stevia rebaudiana residues. The Trichoderma atroviride is fermented by using the method, so that production process links can be greatly reduced; and the Trichoderma atroviride is subjected to solid fermentation by using the method provided by the invention, so that a great number of conidiospores can be generated, the yield per unit is 2.5*10<9> spores, the fermentation unit is high, and the production process is environment-friendly and pollution-free. According to the technical scheme of the invention, not only is a new approach provided for the recycle of stevia rebaudiana, but also a feasible fermentation method is provided for producing the anti-biological inoculant of the Trichoderma atroviride ZYTA09, and the Trichoderma atroviride obtained by fermentation can be applied to a field to play multiple effects of preventing and treating various plant pathogenic fungi, promoting the growth of crops and improving soil.

Description

technical field [0001] The invention relates to a preparation method of a Trichoderma dark green biocontrol agent, in particular to a method for preparing the Dark green Trichoderma biocontrol agent by using stevia rebaudiana slag and fermentation of the dark green Trichoderma biocontrol agent. Background technique [0002] my country is currently the world's largest producer and supplier of stevia sugar products, accounting for about 90% of the global output, but a large amount of stevia residues have not been well utilized, not only causing a great waste of resources, but also seriously polluting the environment. Research data show that stevia rebaudiana dregs are very valuable as feed or additives, but the crude fiber in stevia rebaudiana dregs is high, which is the main factor affecting the large proportion of its addition as feed. Stevia rebaudiana slag contains high organic carbon and trace elements, after proper pretreatment, and adding suitable nutrients, it is used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P3/00A01P21/00C12R1/885
CPCA01N63/30C12N1/14C12N3/00
Inventor 刘振宇郑金媛李祥英尚春晓韦玮
Owner SHANDONG AGRICULTURAL UNIVERSITY
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