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Method for conveniently extracting and detecting rice blast genes

A rice blast and gene technology, which is applied in the field of rice blast gene extraction and detection, can solve problems such as affecting the rice blast gene detection efficiency, affecting the detection effect, slow mixing progress, etc., so as to reduce the generation of foam and ensure normal storage. , the effect of improving efficiency

Inactive Publication Date: 2019-02-22
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that is convenient to extracting the rice blast gene of rice, to solve the above-mentioned background technology, the gene of existing rice blast is mostly extracted directly from the rice plant, thus can be mixed with some impurities, It affects the detection effect in the later stage, and when the gene extraction buffer is mixed with the rice blast plant, the mixing progress is slow, and bubbles will be generated after mixing, which further affects the detection efficiency of the rice blast gene

Method used

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Embodiment 1

[0043] This embodiment 1 provides a method for conveniently extracting and detecting rice blast gene, and the above method will be described in detail below.

[0044] Before extracting the rice blast gene, prepare multiple test tubes for the experiment in advance, and the test tubes are all 2ml centrifuge tubes, and prepare the gene extraction buffer at the same time, wherein the gene extraction buffer uses NaCl, Tris-HCl, EDTA and CTAB In order to ensure the normal use of the gene extraction buffer in the later stage, researchers can store the gene extraction buffer in TE buffer, because TE buffer is weakly alkaline and has protection for the bases of genes (DNA) , so the stability of DNA in TE buffer is better, and it is not easy to destroy its integrity or produce open loops and breaks, thereby ensuring the normal storage of rice blast genes in the later stage and making preparations for subsequent gene extraction and detection;

[0045] Then the researchers cut the disease...

Embodiment 2

[0050] This embodiment 1 provides a method for conveniently extracting and detecting rice blast gene, and the above method will be described in detail below.

[0051] Before extracting the rice blast gene, prepare multiple test tubes for the experiment in advance, and the test tubes are all 2ml centrifuge tubes, and prepare the gene extraction buffer at the same time, wherein the gene extraction buffer uses Tris-HCl, EDTA, ddH2O and TE In order to ensure the normal use of the gene extraction buffer in the later stage, researchers can store the gene extraction buffer in TE buffer, because TE buffer is weakly alkaline and has protection for the bases of genes (DNA) , so the stability of DNA in TE buffer is better, and it is not easy to destroy its integrity or produce open loops and breaks, thereby ensuring the normal storage of rice blast genes in the later stage and making preparations for subsequent gene extraction and detection;

[0052] Then the researchers cut the diseased...

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Abstract

The invention discloses a method for conveniently extracting and detecting rice blast genes. The method includes the steps: firstly, preparing a plurality of storage test tubes and gene extraction buffer solution; secondly, storing the gene extraction buffer solution in TE buffer solution; thirdly, clipping rice blast strains and mixing the gene extraction buffer solution and rice blast plants; fourthly, centrifugally mixing the solution, performing water-curing treatment on the mixed solution and then performing cooling filtration after water-bath heating; fifthly, adding chloroform and isoamyl alcohol and centrifugally mixing the chloroform, the isoamyl alcohol and the solution; sixthly, extracting the solution, injecting the solution into a PCR (polymerase chain reaction) pore plate, and refrigerating the solution in an environment at the temperature of -20 DEG C; finally, performing data acquisition and comparison on the refrigerated mixed solution. According to the method for conveniently extracting and detecting the rice blast genes, smoothness of rice blast gene extraction operation can be ensured, convenience is brought for researchers, and rice blast gene extraction and detection efficiency can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of rice blast detection technology, in particular to a method for conveniently extracting and detecting the rice blast gene. Background technique [0002] Rice is one of the crops. Rice is produced after shelling. Rice is the main food of southern people and is rich in B vitamins. The carbohydrates in rice are mainly starch, and the protein in rice is mainly glutenin. Among them, the biological value of protein and the proportion of amino acids are higher than those of other grains. [0003] Rice has become the staple food of people, and its quality has attracted more and more attention from consumers. Therefore, the research on rice yield has become increasingly hot. However, various rice blasts will occur during the planting process of rice. Rice blast is also known as rice fever, commonly known as fire blast , hanging head blast, neck pinch blast etc. are common important diseases in various rice areas a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6895C12Q1/04
CPCC12Q1/6806C12Q1/6895C12Q2527/125
Inventor 黄永相李伟郭建夫李东祝敏丹
Owner GUANGDONG OCEAN UNIVERSITY
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